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Baits and simple culture method for sea oxyrrhis marina

A method for culturing and a technique for Acerola, applied in the field of experimental marine biology, can solve the problem of the concentration ratio of bait algae species bait algae and the marine hornwort, the difficulty in mastering the cultivation conditions, the easy pollution of bacterial culture, and the lack of requirements for species preservation conditions. Availability, efficiency and environmental friendliness, etc., to achieve the effect of low cost, easy maintenance and simple method

Inactive Publication Date: 2016-07-06
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, various problems have been encountered in the cultivation of marine C. chinensis by using living baits, including: bacterial culture is easy to pollute and requires high species preservation conditions, the species of bait algae, the concentration ratio between bait algae and marine C. Difficult to grasp conditions
There is a lack of a bait that is easy to obtain, economical, efficient and environmentally friendly at the same time

Method used

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  • Baits and simple culture method for sea oxyrrhis marina
  • Baits and simple culture method for sea oxyrrhis marina
  • Baits and simple culture method for sea oxyrrhis marina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Selection of bait. Choose freshly boiled egg yolks as the yolk bait for marine sharpweed;

[0023] 2) Egg yolk bait culture solution mother solution preparation. Use sterilized f / 2 culture medium to prepare mother liquor, cook the whole egg and then carry out the following steps in the ultra-clean workbench: peel off the eggshell, remove the egg white part, weigh the egg yolk quantitatively and put it into a sterilized glass homogenizer , and then inject quantitative sterilized f / 2 culture solution into the glass homogenizer, carefully grind the egg yolk and homogenize it, and then continuously wash the homogenizer with quantitative sterilized f / 2 culture solution, and the homogenate is sterilized by 500-mesh stainless steel cells After sieving, all of them were poured into a sterilized Erlenmeyer flask with a sealing film as the mother liquid of the culture solution and stored in a 4°C refrigerator for later use.

[0024] 3) Preparation of egg yolk bait culture so...

Embodiment 2

[0027] The difference from Example 1 is that: the selected initial egg yolk bait concentration for cultivating the marine sharpweed is 10mg / L, culture conditions: temperature 18°C, light intensity 2000lx, light-dark ratio L:D=10h:14h, salt Degree 4, all the other operating methods and steps are the same as in Example 1. After cultivating for 10 days, a light-colored (light pink) culture solution was obtained, and the density of the marine sharp-tailed algae could reach 1.57×10 5 cell / L. The color changes from milky white to pale pink, which means that under the set culture conditions, the sea algae has been successfully cultured.

Embodiment 3

[0029] The difference from Example 1 is that the egg yolk homogenate selected for cultivating the marine Zengers passes through an 800-mesh stainless steel cell sieve, culture conditions: temperature 20°C, light intensity 4000lx, light-to-dark ratio L:D=14h:10h, Salinity 30, all the other operation methods and steps are identical with embodiment 1. After culturing for 10 days, a light-colored (light pink) culture solution was obtained, and the density of the marine sharp-tailed algae could reach 7.08×10 5 cell / L. The color changes from milky white to pale pink, which means that under the set culture conditions, the sea algae has been successfully cultured.

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Abstract

The invention discloses a simple culture method for sea oxyrrhis marina.The simple culture method includes the steps that cooked yolk of fresh eggs serves as baits, under the culturing conditions that the temperature ranges from 18 DEG C to 25 DEG C, the light intensity ranges from 2,000 lx to 4,000 lx, the light-dark ratio of L to D is (12 h-14 h):(10 h-12 h), the salinity ranges from 4 to 60, the concentration range of the yolk baits ranges from 10 mg / L to 50 mg / L, and culture bottles are shaken once in the morning and the evening every day, long-term culturing of the ea oxyrrhis marina can be achieved.The baits of the method are quite easy to obtain, small in use amount and low in cost, and required instruments and equipment exist in a common laboratory.According to the baits and the simple culture method, the method is simple, maintenance is easy, and high-quality algae species can be provided for the experimental ecology and resource utilization of the sea oxyrrhis marina.

Description

technical field [0001] The invention relates to the field of experimental marine biology, in particular to a simple cultivation method for marine sharpweed, which provides a simple cultivation method for marine sharpweed experimental ecology and its resource utilization. Background technique [0002] Cynocercus marine algae is a kind of red tide microalgae widely distributed in the world. It has become the model marine plankton in the international indoor marine biological experiment. A heterotrophic dinoflagellate that lives omnivorously as its main food. C. marine algae is a heterotrophic marine dinoflagellate. At present, there are few researches on C. marine algae at home and abroad. One of the important reasons why there are few studies on the marine Zengercus is that it is difficult to master the cultivation method. What kind of bait to choose and what kind of cultivation conditions to choose are the keys to breaking through this bottleneck. In China, only the Ocean ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 安鑫龙李志霞李雪梅
Owner HEBEI AGRICULTURAL UNIV.
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