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Preparation method of electrochemical micro-detection system based on disposable pipette tip and lead core electrode array

An electrode array and detection system technology, applied in the field of electrochemical micro-detection system preparation, can solve the problems of large sample volume, unmaintainable, small solution volume, etc., and achieves high accuracy and sensitivity, increased contact surface area, and improved contact area effect

Inactive Publication Date: 2016-07-13
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention is to solve the problem that conventional electrochemical detection systems require a large amount of samples, and the detection cost is expensive; and it can only be recycled, which increases the workload and easily causes secondary pollution, which affects the objectivity of the detection results; and the usual Electrochemical micro-detection systems generally have a planar structure and can only be equipped with false reference electrodes. Electrochemical detection cannot maintain a stable potential, and due to the small volume of the solution in the micro-detection system, the slight volatilization of the solution surface in the sample area causes the solution in the sample area to evaporate. Changes in concentration have a great impact on the technical problems of detection accuracy and sensitivity, and provide a method for preparing an electrochemical micro-detection system based on disposable pipette tips and lead-core electrode arrays

Method used

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  • Preparation method of electrochemical micro-detection system based on disposable pipette tip and lead core electrode array
  • Preparation method of electrochemical micro-detection system based on disposable pipette tip and lead core electrode array
  • Preparation method of electrochemical micro-detection system based on disposable pipette tip and lead core electrode array

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specific Embodiment approach 1

[0029] Embodiment 1: This embodiment is a method for preparing an electrochemical micro-detection system based on a disposable pipette tip and a lead electrode array, specifically as follows:

[0030] 1. Preparation of lead array:

[0031] ①. Divide a pencil lead into three sections a, b and c. a and c are respectively located at both ends of the pencil lead, and b is located in the middle of the pencil lead. Polish section a of the pencil lead with sandpaper so that the polished a The peak-to-peak potential difference is 60mV to 90mV when the section is cyclically scanned in a potassium ferricyanide solution with a concentration of 1mmol / L to 5mmol / L to obtain a polished pencil lead; cover the polished pencil lead with polytetrafluoroethylene The b section of the pencil lead, and then use a copper wire to wind the c section of the pencil lead 10 times to get a lead electrode;

[0032] ② Repeat step 1 to prepare n identical lead-core electrodes, then twist n+1 copper wires wrap...

specific Embodiment approach 2

[0037] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the preparation method of the PBS solution with a pH of 7.4 described in step two is: 8g of NaCl, 0.2g of KCl, 3.63g of 12H 2 O Na 2 HPO 4 and 0.24 g of KH 2 PO 4 Dissolve in 1000mL distilled water and mix well to obtain a PBS solution with a pH of 7.4. Others are the same as the first embodiment.

specific Embodiment approach 3

[0038] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the preparation method of the carbon nanotube modification solution described in step two is: dissolving 20 mg of multi-walled or single-walled carbon nanotubes in 10 mL distilled water, and then sonicated for 1.5 h to obtain a carbon nanotube modification solution. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention provides a preparation method of an electrochemical micro-detection system based on a disposable pipette tip and a lead core electrode array and relates to the preparation method of the electrochemical micro-detection system, aiming at solving the technical problems that a conventional electrochemical detection system is high in preparation cost, and complicated and strict in process, only can be circularly utilized, easily causes secondary pollution and needs more samples to be detected, and an existing electrochemical micro-detection system commonly has a plane structure so that a real reference electrode is difficult to arrange and the accuracy and sensitivity are relatively poor. The preparation method comprises the following steps of: 1, preparing a lead core array; 2, decorating the lead core electrode array; 3, assembling a three-electrode system; 4, preparing an electrolytic cell; and 5, assembling the electrochemical micro-detection system. The invention designs the simple and novel three-dimensional electrochemical micro-detection system based on the disposable pipette tip and a lead core, and the system is uniquely combined with the structure advantages of the real reference electrode; the sensitive detection of micro samples is realized and the accuracy and sensitivity of the electrochemical detection are effectively improved.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical micro-detection system. Background technique [0002] The application of electrochemistry in the biological field has become a hot spot. However, at present, the electrochemical detection of biological samples (such as cells) mainly uses regular-sized electrodes (about 2-4mm in diameter) as working electrodes, and is equipped with corresponding conventional large-sized detection cells, which makes the demand for biological samples large, not only It increases the workload and makes the detection cost expensive. In addition, as high-cost electrodes and detection cells, they can only be recycled. Before each experiment, the surface of the electrodes and the electrolytic cell need to be updated, which also increases the workload and easily leads to secondary pollution. sex affects. The above problems make it difficult for electrochemistry to meet the detection requirements of high accu...

Claims

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Application Information

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IPC IPC(8): G01N27/416
CPCG01N27/416
Inventor 郭晓玲李锦莲周实李秀玲王仲高洪福王伟娥崔继文武冬梅
Owner JIAMUSI UNIVERSITY
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