Preparation method of ethyl ester type fish oil with high EPA content

Abstract

The invention discloses a preparation method of an ethyl ester type fish oil with a high EPA content. The conventional method for separating EPA and DHA has the disadvantages of high cost or complicated operation of separation method, so EPA and DHA in fish oil cannot be separated well or the industrial production cannot be realized. According to the preparation method, crude fish oil is taken as the raw material; at first, lipase with 1,3 specificity is used to carry out alcoholysis reactions; then reduced pressure short term distillation is performed to separate glyceride and ethyl ester; the obtained ethyl ester type fish oil is subjected to rectification and urea adduction to obtain ethyl ester type fish oil with an EPA content more than 80%, and the total omega-3 fatty acid content is more than 90%. The preparation method can effectively separate EPA and DHA, ethyl ester type fish oil with an EPA content more than 80% can be obtained, enzymatic catalysis is used in the technology, and the preparation method has the advantages of low energy consumption, little pollution, and suitability for industrial production.

Description

technical field [0001] The invention relates to the preparation of fatty acids, in particular to a method for preparing high-content EPA ethyl ester type fish oil. Background technique [0002] Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are one of several omega-3 fatty acids commonly used in the human body, and have important physiologically active functions. In our daily diet, the main source of omega-3 fatty acids is fish, especially deep-sea fish. The total content of EPA and DHA in crude fish oil is less than 30%. Currently, methods for enriching EPA and DHA in fish oil mainly include organic solvent extraction, urea inclusion, molecular distillation, low temperature crystallization, metal salt precipitation, and supercritical extraction. Although these methods can increase the content of omega-3 fatty acids in fish oil a lot, the disadvantage is that EPA and DHA, which are the characteristic fatty acids of omega-3 fatty acids, are mixed together , with...

AI Technical Summary

Problems solved by technology

The total content of EPA and DHA in crude fish oil is less than 30%. Currently, methods for enriching EPA and DHA in fish oil mainly include organic solvent extraction, urea inclusion, molecular distillation, low temperature crystallization, metal salt precipitation, and supercritical extraction. Although these methods can increase the content of omega-3 fatty acids in fish oil a lot, the disadvantage is that EPA and DHA, which are the characteristic fatty acids of omega-3 fatty acids, are mixed together , without separation

Although this method can obtain higher-purity EPA and DHA ethyl ester, it needs a large amount of solvent, requires high equipment, high cost, and less processing capacity, so it is difficult to realize industrial-scale production.

[0006] In the above separation methods, either because of the high cost or the cumbersome operation of the separation method, the EPA and DHA in the fish oil cannot be separated well or industrialized production can be realized.