Kit and applications thereof

A kit and probe technology, applied in the field of biomedicine, can solve problems such as the inability to distinguish low-frequency mutations in tumor samples from sequencing errors, the error rate of sequencing technology, and the unfavorable accuracy and sensitivity of early diagnosis of colorectal cancer

Inactive Publication Date: 2016-07-20
天津华大基因科技有限公司 +1
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  • Summary
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Problems solved by technology

Nowadays, high-throughput sequencing technology has been effectively applied in tumor detection. However, due to the low content of plasma cell-free DNA in the early stage of colorectal cancer, and the sequencing technology itself has a certain error rate, traditional sequencing methods will not be able to distinguish Sequencing errors and low-frequency mutations in tumor specimens are detrimental to the accuracy and sensitivity of early diagnosis of colorectal cancer

Method used

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  • Kit and applications thereof
  • Kit and applications thereof
  • Kit and applications thereof

Examples

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Embodiment

[0057] The following specific implementation process takes the peripheral blood plasma of the subject as the research object, and the sample comes from Tianjin Maternal and Child Health Hospital. The content is as follows:

[0058] (1) Separation of peripheral blood samples

[0059] 1. Collect 1-2 tubes (5mL / tube) of peripheral blood from the subject into EDTA anticoagulant tubes, gently invert up and down (to prevent cell rupture) 6-8 times and mix well, and perform the following within 4-6 hours on the day of blood collection deal with;

[0060] 2. Centrifuge at 1600g for 10 minutes at 4°C. After centrifugation, divide the supernatant (plasma) into multiple 1.5mL / 2mL centrifuge tubes. During the absorption process, the middle layer of white blood cells cannot be absorbed;

[0061] 3. Centrifuge at 16,000g for 10 minutes at 4°C to remove residual cells, and transfer the supernatant (plasma) to a new 1.5mL / 2mL centrifuge tube. If the white blood cells cannot be sucked to the ...

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Abstract

The invention provides a kit. The kit comprises a probe, wherein the probe is fixed to a solid-phase substrate or floats in a solution, and can specifically recognize a gene area of at least ten genes among 60 genes in the table 1. The invention further provides applications of the kit, a method for constructing a target area sequencing library, a sequencing method, a method for detecting the target area variation, a device for detecting the target area variation, as well as a method and a device for carrying out early screening on the colorectal cancer. With the adoption of the kit and/or the methods and the devices provided by the invention, the related gene sequences of the colorectal cancer can be easily and conveniently obtained in a high-specific manner at one time, and accurately detected and analyzed, and a detection and analysis result can be used for assisting the early screening judgment or monitoring on the colorectal cancer.

Description

technical field [0001] The present invention relates to the field of biomedicine, specifically, to a kit and its use, more specifically, the present invention relates to a kit, the use of the kit, a method for constructing a sequencing library of a target region, a sequencing method, a A method and device for detecting variation in a target region, and a method and device for early screening of colorectal cancer. Background technique [0002] Tumor is one of the diseases that seriously threaten human health. Since the 1970s, the incidence and mortality of cancer in my country have been on the rise. In the 20 years to the 1990s, the cancer mortality rate increased by 29.42%, and the age-adjusted mortality rate increased by 11.56%. In 2000, the incidence of cancer was about 1.8-2 million, and the death rate was 1.4-1.5 million. The incidence of malignant tumors nationwide is 87.07 / 100,000 for the age group of 35 to 39 years old, almost doubled to 154.53 / 100,000 for the age gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12Q1/68C12M1/00C12M1/34C40B50/06
Inventor 蔡宇航杨玲陈希管彦芳朱晨晨戴平平朱红梅易鑫
Owner 天津华大基因科技有限公司
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