Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating polymyxin E fermentation liquid by multistage ceramic membrane microfiltration

A ceramic membrane microfiltration, polymyxin technology, applied in the directions of polymyxin, chemical instruments and methods, specific peptides, etc., to achieve the effects of low energy consumption, shortened production cycle, and high degree of automation

Inactive Publication Date: 2016-08-03
ANHUI NORMAL UNIV +1
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcomings of the current separation process are: the lack of a separation method for polymyxin E fermentation broth that is refined in process steps, easy to operate, low in cost, and environmentally safe.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Take 2000L of fermentation broth containing polymyxin E at a concentration of 600,100 U / mL as raw material, introduce it into an acidification tank, add sulfuric acid to adjust the pH value to 2.7, and keep stirring for 50 minutes (40-60 minutes is acceptable).

[0031] 2) Separate the acidified fermentation broth through the first-stage ceramic membrane microfiltration system, using 6 layers of ceramic membranes with a pore size of 50 nanometers, and the membrane flux is 120L / m 2 h, the operating temperature was 30°C, and the operating pressure was 0.4 MPa, to obtain 4,800 L of the first-stage filtrate, and the concentration of polymyxin E was 248,500 U / mL.

[0032] Pass 4800L of the first-stage filtrate into the second-stage ceramic membrane microfiltration system for separation, using 4 layers of ceramic membranes with a pore size of 5 nanometers, and the membrane flux is 40L / m 2 h, the operating temperature was 32°C, the operating pressure was 0.8 MPa, and the se...

Embodiment 2

[0034] 1) Take 2000L of fermentation broth containing polymyxin E at a concentration of 652,300 U / mL as raw material, introduce it into an acidification tank, add sulfuric acid to adjust the pH value to 2.7, and keep stirring for 50 minutes (40-60 minutes is acceptable).

[0035] 2) Separate the acidified fermentation broth through the first-stage ceramic membrane microfiltration system, using 6 layers of ceramic membranes with a pore size of 50 nanometers, and the membrane flux is 130L / m 2 h, the operating temperature was 31°C, and the operating pressure was 0.4 MPa, to obtain 4600 L of the first-stage filtrate, and the concentration of polymyxin E was 282,100 U / mL.

[0036] Pass 4600L of the first-stage filtrate into the second-stage ceramic membrane microfiltration system for separation, using 4 layers of ceramic membranes with a pore size of 5 nanometers, and the membrane flux is 40L / m 2 h, the operating temperature was 32°C, the operating pressure was 0.9MPa, and the seco...

Embodiment 3

[0038] 1) Take 2000L of fermentation broth containing polymyxin E at a concentration of 625,500 U / mL as raw material, introduce it into an acidification tank, add sulfuric acid to adjust the pH value to 2.7, and keep stirring for 50 minutes (40-60 minutes is acceptable).

[0039] 2) Separate the acidified fermentation broth through the first-stage ceramic membrane microfiltration system, using 6 layers of ceramic membranes with a pore size of 50 nanometers, and the membrane flux is 120L / m 2 h, the operating temperature was 32°C, and the operating pressure was 0.5 MPa, to obtain 4600 L of the first-stage filtrate, and the concentration of polymyxin E was 270,300 U / mL.

[0040] Pass 4600L of the first-stage filtrate into the second-stage ceramic membrane microfiltration system for separation, using 4 layers of ceramic membranes with a pore size of 5 nanometers, and the membrane flux is 40L / m 2 h, the operating temperature was 33°C, the operating pressure was 0.9MPa, and the seco...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for separating a polymyxin E fermentation liquid by multistage ceramic membrane microfiltration. The method comprises the following steps: 1) acidizing the polymyxin E fermentation liquid; and 2) sequentially passing the acidized polymyxin E fermentation liquid through the multistage ceramic membrane microfiltration system to perform microfiltration separation, wherein the pore sizes of the ceramic membranes in the ceramic membrane microfiltration system gradually decrease along the polymyxin E fermentation liquid microfiltration separation direction. The method has the advantages of mild conditions and low worker labor intensity, reduces the separation process steps and enhances the separation recovery yield.

Description

technical field [0001] The invention relates to the separation of polymyxin E fermentation liquid, in particular to a method for separating polymyxin E fermentation liquid by adopting multistage ceramic membrane microfiltration. Background technique [0002] Polymyxin E, also known as colistin, polymyxinsulfate, is a basic cyclic polypeptide antibiotic produced by Bacillus polymyxa. Polymyxin E is amorphous white powder, high temperature resistant, easily soluble in water, stable in nature, and can effectively inhibit the proliferation of various Gram-negative bacteria, especially for Pseudomonas aeruginosa, Salmonella, Shigella, aerogenes and Klebsiella pneumoniae have significant effects. Polymyxin E can attach to the surface of bacteria, and the hydrophobic functional groups in it can combine with the lipoprotein phosphate in the bacterial cell membrane to destroy the integrity of the outer membrane, increase the permeability of the entire cell membrane, and lead to Sma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K7/62C07K1/34
CPCC07K7/62
Inventor 林凌蒋顺进黄炜乾朱国萍
Owner ANHUI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com