Method for separating polymyxin E fermentation liquid by multistage ceramic membrane microfiltration

A ceramic membrane microfiltration, polymyxin technology, applied in the directions of polymyxin, chemical instruments and methods, specific peptides, etc., to achieve the effects of low energy consumption, shortened production cycle, and high degree of automation

Inactive Publication Date: 2016-08-03
ANHUI NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcomings of the current separation process are: the lack of a separation method for polymyxin E fe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1

[0030] 1) Take 2000L of fermentation broth with a concentration of 601,000 U / mL of polymyxin E as raw material, introduce it into the acidification tank and add sulfuric acid to adjust the pH to 2.7, and continue to stir for 50min (40-60min is acceptable).

[0031] 2) The acidified fermentation broth is separated by the first-stage ceramic membrane microfiltration system, using 6 layers of ceramic membranes with a pore size of 50 nanometers, and the membrane flux is 120L / m 2 ·H, the operating temperature is 30℃, the operating pressure is 0.4MPa, the first-stage filtrate is 4800L, and the polymyxin E concentration is 248,500 U / mL.

[0032] Pass the 4800L first-stage filtrate into the second-stage ceramic membrane microfiltration system for separation, using 4 layers of ceramic membranes with a pore size of 5 nanometers, and the membrane flux is 40L / m 2 ·H, the operating temperature is 32°C, the operating pressure is 0.8MPa, the second-stage filtrate is 10000L, the po...

Example Embodiment

[0033] Example 2

[0034] 1) Take 2000L of fermentation broth with a concentration of 652,300 U / mL of polymyxin E as raw material, introduce it into an acidification tank, add sulfuric acid to adjust the pH to 2.7, and continue to stir for 50 minutes (40-60 minutes can be used).

[0035] 2) Separate the acidified fermentation broth through the first-stage ceramic membrane microfiltration system, using 6 layers of ceramic membranes with a pore size of 50 nanometers, and the membrane flux is 130L / m 2 ·H, the operating temperature is 31°C, the operating pressure is 0.4MPa, the first-stage filtrate is 4600L, and the polymyxin E concentration is 282,100 U / mL.

[0036] Pass the 4600L first-stage filtrate into the second-stage ceramic membrane microfiltration system for separation, using 4 layers of ceramic membranes with a pore size of 5 nanometers, and the membrane flux is 40L / m 2 ·H, the operating temperature is 32℃, the operating pressure is 0.9MPa, the second-stage filtrate is 9800L, th...

Example Embodiment

[0037] Example 3

[0038] 1) Take 2000L of fermentation broth with a concentration of 625,500 U / mL of polymyxin E as raw material, introduce it into an acidification tank, add sulfuric acid to adjust the pH to 2.7, and continue to stir for 50 minutes (40-60 minutes are acceptable).

[0039] 2) The acidified fermentation broth is separated by the first-stage ceramic membrane microfiltration system, using 6 layers of ceramic membranes with a pore size of 50 nanometers, and the membrane flux is 120L / m 2 ·H, the operating temperature is 32℃, the operating pressure is 0.5MPa, the first-stage filtrate is 4600L, and the polymyxin E concentration is 270,300 U / mL.

[0040] Pass the 4600L first-stage filtrate into the second-stage ceramic membrane microfiltration system for separation, using 4 layers of ceramic membranes with a pore size of 5 nanometers, and the membrane flux is 40L / m 2 ·H, the operating temperature is 33°C, the operating pressure is 0.9MPa, the second-stage filtrate is 10500L,...

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Abstract

The invention discloses a method for separating a polymyxin E fermentation liquid by multistage ceramic membrane microfiltration. The method comprises the following steps: 1) acidizing the polymyxin E fermentation liquid; and 2) sequentially passing the acidized polymyxin E fermentation liquid through the multistage ceramic membrane microfiltration system to perform microfiltration separation, wherein the pore sizes of the ceramic membranes in the ceramic membrane microfiltration system gradually decrease along the polymyxin E fermentation liquid microfiltration separation direction. The method has the advantages of mild conditions and low worker labor intensity, reduces the separation process steps and enhances the separation recovery yield.

Description

technical field [0001] The invention relates to the separation of polymyxin E fermentation liquid, in particular to a method for separating polymyxin E fermentation liquid by adopting multistage ceramic membrane microfiltration. Background technique [0002] Polymyxin E, also known as colistin, polymyxinsulfate, is a basic cyclic polypeptide antibiotic produced by Bacillus polymyxa. Polymyxin E is amorphous white powder, high temperature resistant, easily soluble in water, stable in nature, and can effectively inhibit the proliferation of various Gram-negative bacteria, especially for Pseudomonas aeruginosa, Salmonella, Shigella, aerogenes and Klebsiella pneumoniae have significant effects. Polymyxin E can attach to the surface of bacteria, and the hydrophobic functional groups in it can combine with the lipoprotein phosphate in the bacterial cell membrane to destroy the integrity of the outer membrane, increase the permeability of the entire cell membrane, and lead to Sma...

Claims

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Application Information

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IPC IPC(8): C07K7/62C07K1/34
CPCC07K7/62
Inventor 林凌蒋顺进黄炜乾朱国萍
Owner ANHUI NORMAL UNIV
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