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Piston sample rack for gene gun

A sample rack and gene gun technology, applied in the field of sample racks, can solve problems such as being easily blown away, large air flow, and affecting the hitting efficiency of gene guns, and achieve the effects of reducing airflow impact, increasing hit rate, and increasing friction

Active Publication Date: 2017-12-19
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when using a glass dish or a closed sample chamber to transform powdery materials such as pollen, a large airflow will be generated after the gene gun is fired.
Due to its light weight and small size, the powdered receptor material is easily blown away after being impacted by a strong airflow, thus affecting the hitting efficiency of the gene gun

Method used

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  • Piston sample rack for gene gun
  • Piston sample rack for gene gun

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Embodiment Construction

[0016] The present invention will be described in detail below with reference to the accompanying drawings and embodiments.

[0017] like figure 1 , figure 2 As shown, the present invention proposes a piston sample holder for a gene gun, which includes a cylinder 1 with two ends open and arranged vertically, a piston 2 is slidably arranged on the inner wall of the cylinder 1, and the piston 2 passes through a plurality of intervals Arranged support rods 3 are connected to a cup body 4 for containing the receptor material.

[0018] In the above embodiment, as figure 1 As shown, a slide 6 with a hole 5 is arranged at the bottom of the cup body 4, and the aperture of the hole 5 is smaller than the acceptor material, which is beneficial to the discharge of the gas in the cup body 4 and avoids the rapid air in the cylinder body 1 after the gene gun 7 is shot. swell. A frosted surface is provided on the inner side of the carrier sheet 6 to increase the friction force between th...

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Abstract

The invention relates to a piston sample rack for a gene gun. The piston sample rack is characterized by comprising a vertical cylinder with the two ends open; a piston is arranged on the inner wall of the cylinder in a sliding mode, and the piston is connected with a cup used for containing an acceptor material through a plurality of supporting rods arranged at intervals. A slide with holes is arranged at the bottom of the cup, and a frosted surface is arranged on the inner side of the slide. A plurality of through holes are formed in the part, above the cup, of the side wall of the cylinder. Sliding ways are arranged on the inner wall of the cylinder, and the piston and the sliding ways are connected in a sliding mode. A gap is formed between the cylinder and the cup. A supporting frame is arranged on the outer wall of the lower portion of the cylinder. A stretching-out end is arranged at the bottom of each sliding way. Scale mark values are arranged on the outer wall of the cylinder. By means of the piston sample rack, gas flow impact to the acceptor material can be relieved, the acceptor material is prevented from being blown off, and thus the hit efficiency of the gene gun is improved; the piston sample rack is simple in structure and convenient to use.

Description

technical field [0001] The present invention relates to a sample holder, in particular to a piston sample holder for a gene gun. Background technique [0002] Gene gun method is a brand-new gene transfer technology, which directly injects high-speed microprojectiles attached with genetic material or other substances into cells, tissues and organelles. Efficient features. The gas gene gun is powered by a gas shock wave converted from compressed gas (helium or nitrogen), and sends a "cold" gas shock wave generated by the gas gene gun into the bombardment chamber, thereby avoiding the cell damage caused by the "hot" gas shock wave. The gas gene gun can achieve transient, stable and efficient transformation in a wide range of cell types. The gas gene gun has a special structure that generates shock waves. It has correspondingly different rupturable membranes within the appropriate pressure range from 3.5 to 10 MPa, and penetrates the DNA-coated particles, and finally shoots th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCC12M35/04
Inventor 阴祖军叶武威李燕姜辉耿月华王俊娟
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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