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A rhizosphere growth-promoting Bacillus arzii for blueberries and its application

A technology of Bacillus aeruginosa and blueberry, applied in the field of rhizosphere growth-promoting Bacillus aeruginosa, can solve the problems of lack of PGPR strain resources, difficult to survive and colonize, unable to produce continuous and stable efficacy, etc., and achieve excellent strain resources. , The effect of promoting blueberry growth and accelerating blueberry rooting

Inactive Publication Date: 2017-10-17
SHANDONG FOREST SCI RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Blueberry has a shallow root system without root hairs. The roots are mostly distributed in shallow soil, and the growth environment is relatively special. Commonly used functional microorganisms are difficult to survive and colonize when inoculated into blueberry soil, and cannot produce sustained and stable effects. Based on the above reasons, The resources of PGPR strains suitable for blueberries are very scarce. Continuing to enrich the resources of blueberry strains is of great significance for improving the micro-ecological environment of blueberry roots, promoting the growth of blueberries, and improving the quality of blueberries

Method used

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  • A rhizosphere growth-promoting Bacillus arzii for blueberries and its application
  • A rhizosphere growth-promoting Bacillus arzii for blueberries and its application
  • A rhizosphere growth-promoting Bacillus arzii for blueberries and its application

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Embodiment 1

[0032] Embodiment 1 The screening of Bacillus aryabhattai (Bacillus aryabhattai) BPR078 of the present invention

[0033] (1) Screening process

[0034] Three samples of blueberry rhizosphere soil were collected in different greenhouses in Qingdao Jiaonan Joyvio blueberry planting base. Bacterial isolates were isolated from rhizosphere soil samples by serial dilution method. Purify the isolated bacteria by the three-section line method, and judge whether the strain is purified by microscopic examination, number the purified bacteria, pick a single colony, and transfer it to an LB slant for future use.

[0035] Inoculate the bacteria into the R2A liquid medium containing L-tryptophan, culture at 28°C, 180rpm, on a shaker for 4 days. Take 50 microliters of bacterial suspension and drop it on a white ceramic plate, and add 50 microliters of Salkowski colorimetric solution (50ml 35% HCLO 4 +1ml0.5mol / LFeCl 3 ). The colorimetric solution added with 50 mg / L IAA was used as a po...

Embodiment 2

[0047] Embodiment 2 has growth-promoting effect on blueberry growth

[0048] Activate the BPR078 stored on the slant in LB (peptone 10g; NaCl 10g; yeast extract 5g; agar 15-20g; water 1000ml; pH 7.0-7.2, aliquot and sterilize at 121°C for 20min ) on the tablet. Pick a single colony into the corresponding 10ml liquid medium, and culture at 37°C and 180rpm for 24h. The seed solution was inoculated into corresponding 50ml liquid medium for fermentation according to 1% inoculum amount, and cultured at 37°C and 180rpm for 2 days to arrange the plug-in-plate seedling test of blueberry tissue culture seedlings.

[0049] The hole plate seedling test was carried out on April 30, 2015 at Qingdao Wolin Blueberry Production Base. Blueberry tissue culture seedlings were selected, and each hole plate was treated as one treatment, and 200 tissue culture seedlings were cut. Apply 100mL growth-promoting bacteria fermentation broth to each treatment, dilute to 1000mL and water evenly, manage ...

Embodiment 3

[0057] A kind of microbial mixture, it is characterized in that comprises bacterial strain Bacillus arsonii (Bacillus aryabhattai) BPR078 of the present invention, also comprises other microorganisms, and described other microorganisms is to promote blueberry growth and does not produce antagonistic effect with Bacillus aryabhattai (Bacillus aryabhattai) BPR078 of microorganisms. It has been verified by experiments that the microbial mixture can obviously promote the rooting of blueberry cuttings, promote the growth of blueberries, and can increase the activities of protease and sucrase in the rhizosphere soil of blueberries.

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Abstract

The invention discloses a rhizosphere growth-promoting Bacillus arbihattai specially used for blueberries and its application. The strain is named Bacillus aryabhattai, numbered BPR078, and has been preserved in China Microorganisms on January 7, 2016. General Microbiology Center of Species Preservation Committee, the deposit number is CGMCC No.11971. The blueberry rhizosphere growth-promoting Bacillus arbii of the present invention is inoculated into blueberry cutting seedlings after being prepared as bacterial agent, which can obviously accelerate blueberry rooting and promote the growth of cutting seedlings, and the difference reaches a significant level compared with the control. Therefore, the present invention provides excellent bacterial strain resources for the future development of blueberry-specific microbial fertilizers.

Description

technical field [0001] The invention relates to a rhizosphere growth-promoting bacillus arborii specially used for blueberries and an application thereof. Background technique [0002] Blueberry (Vacciniumssp.L.) fruit is bright in color and unique in flavor. It contains a lot of protein, fat, vitamins, pectin, SOD, flavonoids, potassium, phosphorus, calcium and other rich nutrients; it contains a lot of natural pigments and has been tested Studies have found that among many kinds of fruits, its pigment content is the highest, and it has become the raw material of health care medicine. [0003] Plant rhizosphere growth-promoting bacteria refer to the beneficial bacteria that live in the rhizosphere of plants, promote plant growth or antagonize pathogenic bacteria, and play an extremely important role in plant growth and disease control. According to incomplete statistics, it has been found that more than 20 genera of plant rhizosphere growth-promoting bacteria, including Ps...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P21/00A01G17/00A01G7/06C12R1/07
CPCA01G7/06A01G17/005A01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 杜秉海马海林丁延芹刘方春姚良同杜振宇范玲超马丙尧徐文凤李丽刘幸红彭琳
Owner SHANDONG FOREST SCI RES INST
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