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Molecular markers closely linked to downy mildew resistance genes of melon and their application

A downy mildew resistance and molecular marker technology, applied in the field of agricultural biology, can solve the problems of long genetic distance and difficult application of molecular marker-assisted breeding, so as to shorten the breeding cycle and improve the selection accuracy

Active Publication Date: 2019-04-26
XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The molecular marker research on the source of resistance was only reported by the Zhengzhou Fruit Tree Institute of the Chinese Academy of Agricultural Sciences respectively against the parent DM3 and PI414723, and found 1 SRAP marker and 3 SSR markers linked to it, but the genetic distance is too far, and the molecular markers Difficult to apply in assisted breeding

Method used

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  • Molecular markers closely linked to downy mildew resistance genes of melon and their application
  • Molecular markers closely linked to downy mildew resistance genes of melon and their application
  • Molecular markers closely linked to downy mildew resistance genes of melon and their application

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Example 1: Molecular markers closely linked to downy mildew resistance genes in melon

[0057] A molecular marker closely linked to the melon downy mildew resistance gene specifically includes the following steps:

[0058] (1) Cross the susceptible farm variety "Karaksai" as the female parent and the disease-resistant resource PI438685 as the male parent to obtain the hybrid F 1 .

[0059] (2) by hybrid F 1 Self-pollinated to obtain F 2 Generation group, F 1 Crossed with "Karaksai" to obtain backcross population BC S , F 1 Cross with PI438685 to obtain backcross population BC r .

[0060] (3) Respectively in the seedling stage to PI438685, "Karaksai", F 1 , BC S , BC r And F 2 Conduct identification of downy mildew resistance.

[0061] The inoculation solution of downy mildew fungus: collect the early diseased leaves from the field, use a soft brush to remove the sporangia on the back of the leaf, place it in a beaker filled with sterile water, and inoculate the infected leaves w...

Embodiment 2

[0070] Example 2: Molecular marker SSR-666 closely linked to the downy mildew resistance gene of melon 230 Applications

[0071] The present invention provides a molecular marker SSR-666 closely linked to the muskmelon downy mildew resistance gene 230 The application of the molecular marker SSR-666 230 Genotype testing for melon varieties or strains to determine whether the varieties or strains are resistant to melon downy mildew, including the following steps:

[0072] (1) Cross the disease-resistant resource PI438685 with other melons and reproduce to F 2 Generation above.

[0073] (2) Extract genomic DNA from a single melon plant obtained in step (1), and use SSR-666 230 This marker is amplified by PCR to detect whether only the unique 230bp specific band appears. If only this specific band appears, it is predicted that the melon plant is resistant to downy mildew.

Embodiment 3

[0074] Example 3: SSR molecular marker SSR-666 linked to melon downy mildew resistance gene 230 The acquisition.

[0075] (1) Cross the disease-resistant material PI438685 as the male parent and Xinjiang thick-skin melon "Karaksai" as the female parent to obtain F 1 , F 1 Selfing produces F 2 , F 1 Hybridize with PI438685 to get BCr, F 1 Cross with "Karaksai" to get BCs. Through the parents, F 1 , F 2 , BCr, BCs were artificially inoculated and the incidence of each individual plant was counted: BCs, F 1 And "Karaksai" all showed susceptibility, PI438685 showed resistance, and the ratio of resistance to susceptibility in BCr was approximately 1:1, F 2 The statistics of the segregated populations of generations showed that among the 197 strains, 53 strains were resistant to the disease and 144 strains were susceptible. The resistance-to-susceptibility ratio was in accordance with 1:3. Therefore, the PI438685 downy mildew resistance gene was determined to be single-gene recessive.

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Abstract

The invention discloses a molecular marker closely linked with a melon pseudoperonospora cubensis resisting gene and the application thereof. Based on the seedling-stage resource pseudoperonospora cubensis resistance identification and selection principle and the prior art, a melon resource PI438685 with high pseudoperonospora cubensis resistance is selected as the high-resistance resource, a melon pseudoperonospora cubensis anti-fever F2-generation group is established by means of the high-resistance resource PI438685 and the susceptible farm variety Clarke, the molecular marker SSR-666230 linked with the PI438685 pseudoperonospora cubensis resisting gene is found by means of well-known heredity software, and the genetic distance between the marker and the resistance gene is 2.66 cm. By extracting genome DNA of a single melon plant and conducting PCR amplification with the marker SSR-666230, whether a unique 230bp specific band appears is detected, it is predicted that the single melon plant has pseudoperonospora cubensis resistance if yes, the technical effects of shortening breeding period and improving selection accuracy during pseudoperonospora cubensis resisting strain breeding are realized, and practical value is high.

Description

Invention field [0001] The invention relates to the field of agricultural biotechnology. Specifically, the present invention relates to the technical field of molecular markers closely linked to the new gene of melon downy mildew resistance and its application. Background technique [0002] Melon is an important economic crop. The planting area and output of melon in my country rank first in the world. Melon downy mildew [Pseudoperonospora cubensis (Berk.etCurt.) Rostov.] under the conditions of high temperature and high humidity, has a rapid prevalence, serious damage, and difficulty in prevention and control. It has a direct impact on the yield of melon, the sugar content of the fruit and the commodity rate. Impact is a devastating disease. Chemical prevention and control of downy mildew is not only inefficient, but also easy to cause environmental pollution, and cultivating disease-resistant varieties is one of the safest, effective and cost-saving measures to prevent and co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张学军李寐华杨永张红张永兵伊鸿平王豪杰
Owner XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT