Fluorescent latex immunochromatography detection reagent for detecting falciparum malaria and preparation method thereof

Abstract

The invention provides a fluorescent latex immunochromatography detection reagent for detecting falciparum malaria and a preparation method thereof. The fluorescent latex immunochromatography detection reagent comprises a sample mat, a combining mat, a reaction film and a water absorption mat. The combining mat is coated with an anti-HRP-2 monoclonal antibody marked by fluorescent latex and anti-rabbit IgG, a detecting line and a quality control line are arranged on the reaction film, the detecting line is coated with an anti-HRP-2 monoclonal antibody, and the quality control line is coated with rabbit IgG. The fluorescent latex immunochromatography detection reagent for detecting falciparum malaria can fast detect plasmodium falciparum, and is fast to operate, low in cost and suitable for clinical use and field use.

Description

technical field [0001] The invention relates to the field of disease detection, in particular to a fluorescent latex immunochromatographic detection reagent for detecting falciparum malaria and a preparation method thereof. Background technique [0002] Malaria is an acute parasitic disease caused by Plasmodium infection that seriously endangers the health of the population. It is transmitted through the bite of Anopheles mosquitoes. In the human body, it undergoes asexual reproduction in the liver and sexual reproduction in the red blood cells, destroying red blood cells in batches and causing disease. There are four kinds of Plasmodium that parasitize the human body: Plasmodium vivax (P.lasmodium), Plasmodium falciparum (P.falciparum), Plasmodium three days (P.malarial) and ovale Plasmodium (P.ovale). Plasmodium vivax and Plasmodium falciparum are common in my country, and only a few cases of Plasmodium ovale have been found. Malaria diagnosis is an important part of ...

AI Technical Summary

Problems solved by technology

Malaria diagnosis is an important part of malaria prevention and control work. At present, the traditional microscopic examination method is still the "gold standard" of malaria diagnosis method. Using the thick blood film method to inspect 100 fields of view can theoretically detect 4 Plasmodium / μl, But its disadvantage is that it is time-consuming and labor-intensive, and at the same time, the inspector needs to have professional knowledge and experience

The blood buffy coat quantitative method (QBC technology) developed in recent years has the disadvantages that the capillary used is expensive, cannot be reused, and the operation is cumbersome, and it is difficult to effectively identify insect species and count protozoa, so it is not applicable Diagnosis of a single malaria patient

However, DNA probe technology and polymerase chain reaction (PCR) and other methods developed in recent years have certain limitations when used in the field, require high operating technology and complex equipment, and are not suitable for traditional methods. Large scale field application

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