High performance liquid chromatography detection method for pyrazine compounds in Baijiu
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A technology of high performance liquid chromatography and detection method, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of large sample consumption, large loss of analyte, cumbersome steps, etc., and achieves low cost, good accuracy and repeatability. , the effect of simple operation
Inactive Publication Date: 2016-08-10
GUIZHOU PROVINCIAL PRODUCT QUALITY SUPERVISION AND INSPECTION INSTITUTE
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However, the above methods require a large amount of organic reagents, a large amount of samples, and the steps are too cumbersome, and the loss of analytes in the pretreatment process is relatively large. For the detection method of pyrazine in liquor by high performance liquid chromatography-fluorescence detector, there is no to report
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[0124] The high-performance liquid chromatography detection method of pyrazine compound in liquor, comprises the following steps:
[0125] Chromatographic conditions: Chromatographic column: phenyl column Gemini 5μC 6 -Phenyl 110A 250mm×4.6mm; mobile phase consists of mobile phase A and mobile phase B, mobile phase A: phosphoric acid aqueous solution with pH=2.1; mobile phase B: acetonitrile; gradient elution mobile phase setting: calculated by volume, 0min : mobile phase A 95%, mobile phase B 5%; 6min: mobile phase A 95%, mobile phase B 5%; 6.1min: mobile phase A 1%, mobile phase B 99%; 12min: mobile phase A 1% , mobile phase B 99%; 12.1min: mobile phase A 83%, mobile B 17%; 12.1min: mobile phase A 83%, mobile B 17%; 12.1min: mobile phase A 83%, mobile B 17%; 16min : mobile phase A 83%, mobile B 17%; 16.1min: mobile phase A 95%, mobile B 5%; 25min: mobile phase A 95%, mobile B 5%; flow rate: 1.0mL / min; column temperature: 30 ℃; Injection volume: 10 μL;
[0126] Fluorescenc...
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Abstract
The invention discloses a high performance liquid chromatography detection method for pyrazine compounds in Baijiu. According to the method, a high performance liquid chromatography-fluorescence detector (HPLC-FLD) method is adopted to perform qualitative and quantitative analysis on the pyrazine compounds in Baijiu, and 2-methylpyrazine, 2, 3-dimethylpyrazine, 2, 5-dimethylpyrazine, 2, 6-dimethylpyrazine, 2, 3, 5-trimethyl pyrazine, 2-ethyl-6-methylpyrazine, 2, 3, 5, 6-tetramethylpyrazine and 2, 3-dimethyl-5-ethylpyrazine can be measured at the same time. With the adoption of the method, 3 types of isomeride can be separated without chemical derivation, chiral columns or adding chiral reagents, is high in sensitivity, good in accuracy and repeatability, simple to operate, and low in cost.
Description
technical field [0001] The invention relates to a high performance liquid chromatography detection method for pyrazine compounds in liquor, belonging to the technical field of liquor detection. Background technique [0002] Pyrazine is a six-membered heterocyclic compound containing 1 and 4 nitrogen, and its derivatives are widely found in natural and fermented foods. In recent years, studies on flavor substances in liquor have found that although the content of pyrazine compounds in liquor is very small, it has the characteristics of low flavor threshold, good aroma permeability, and obvious contrast and superposition effect on other aroma substances. Plays an important role in the flavor of liquor. Tetramethylpyrazine (Ligustrazine) in liquor is produced by Maillard reaction, produced in the process of koji making and stacking fermentation, and brought into the wine through distillation, which has the functions of dilating blood vessels, improving microcirculation and inh...
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