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The use of ablim3 gene as a marker for the diagnosis and treatment of esophageal cancer

A technology for esophageal cancer and genes, which is applied in the detection/testing of microorganisms, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of unsatisfactory effect and low survival rate of advanced esophageal cancer

Active Publication Date: 2019-07-05
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional treatment method is mainly surgery, supplemented by radiotherapy and chemotherapy. Despite the improvement of various treatment techniques, the overall 5-year survival rate is still very low, especially for advanced esophageal cancer.

Method used

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  • The use of ablim3 gene as a marker for the diagnosis and treatment of esophageal cancer
  • The use of ablim3 gene as a marker for the diagnosis and treatment of esophageal cancer
  • The use of ablim3 gene as a marker for the diagnosis and treatment of esophageal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Differences in the expression of ABLIM3 gene in normal esophageal tissue and esophageal cancer tissue

[0060] 1. Experimental materials:

[0061] The esophageal cancer tissue is the specimen removed by the hospital's thoracic surgery, and the normal tissue is the specimen taken out under the microscope of the gastroscope room. Cut with a scalpel blade.

[0062] All cases were confirmed pathologically. Including 40 cases of esophageal cancer tissue and 30 cases of normal esophageal tissue. Among them, there were 34 cases of esophageal squamous cell carcinoma and 6 cases of esophageal adenocarcinoma. Normal esophageal tissue was used as the control group. All patients were pathologically confirmed before operation, and no radiotherapy, chemotherapy and other treatments were performed before operation, and no tumors in other parts were found. All tissues were stored in liquid nitrogen within half an hour of the operation, and then transferred to a -80°C ref...

Embodiment 2

[0120] Embodiment 2 ABLIM3 gene expression plasmid construction

[0121] 1. Construction of ABLIM3 gene expression vector

[0122] Amplification primers were designed according to the coding sequence of the ABLIM3 gene (as shown in SEQ ID NO.1). Amplify the coding sequence of the full-length ABLIM3 gene from the cDNA library of adult fetal brain (clontech company, article number: 638831), insert the above cDNA sequence into the eukaryotic cell expression vector pcDNA3.1, and connect the obtained recombinant vector pcDNA3.1 -ABLIM3 was used in subsequent experiments.

[0123] 2. Culture and transfection of esophageal cancer cells

[0124] 2.1 Cell culture

[0125] The esophageal cancer cell line ECA109 was inoculated in DMEM medium containing 10% fetal bovine serum, and placed at 37°C, 5% CO 2 Cultivate in an incubator, and when the cells reach 80% confluence, they are digested and passaged with 0.25% trypsin.

[0126] 2.2 Cell transfection

[0127] Esophageal cancer cell...

Embodiment 3

[0140] Example 3 Effect of ABLIM3 gene on the proliferation of esophageal cancer cells

[0141] MTT assay was used to detect the effect of ABLIM3 gene on the proliferation ability of esophageal cancer cells.

[0142] 1. Steps: trypsinize each group of cells 12 hours after transfection, make a single cell suspension, inoculate 6,000 cells per well in a 96-well culture plate, set 3 time points for each group, and set 6 times for each time point. multiple holes. After the cells adhere to the wall, perform the first test: add 20 μl of 5g / L MTT solution to each well, continue to cultivate for 4 hours, aspirate the medium, add 150 μl of DMSO, and carefully blow and beat to fully dissolve the purple-blue precipitate. The instrument measures the absorbance value (A value) at a wavelength of 490nm. Then every 24h detection 1, continuous measurement 72h, a total of 3 times.

[0143] 2. Statistical methods

[0144] The experiments were all repeated three times, and SPSS13.0 statistic...

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Abstract

The invention discloses a molecular marker-ABLIM3 gene and its expression product for early diagnosis of esophageal cancer. The present invention uses QPCR and Western blot methods to prove that the ABLIM3 gene is differentially expressed in esophageal cancer tissue and normal esophageal tissue, and can be used as an index for early diagnosis of esophageal cancer. In addition, the present invention also discloses that the ABLIM3 gene and its expression product can be used as a target for the treatment of esophageal cancer and used to guide the research and development of new drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of ABLIM3 gene in the diagnosis and treatment of esophageal cancer. Background technique [0002] Esophageal cancer is a common malignant digestive tract tumor in my country, ranking sixth in the death rate of various cancers. The 2008 World Cancer Incidence and Death Report (Globocan 2008) released by the International Agency for Research on Diseases reported that there were 259,200 cases of esophageal cancer in China, and 481,600 cases of esophageal cancer in the world, accounting for more than half of the global percentage as high as 53.82%, and 406,500 global deaths in the same period 211,100 cases in China, accounting for 51.92%. And with the improvement of living standards and the change of lifestyle, there is an increasing trend year by year. Therefore, the research on the early detection and early diagnosis and treatment of esophageal cancer, especially the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/574A61K45/00A61P35/00
CPCA61K45/00C12Q1/6886C12Q2600/158G01N33/57407G01N33/68G01N2333/47
Inventor 田子强温士旺李振华徐延昭
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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