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Raman spectrum acquisition system with low-fluorescence background

A technology of Raman spectroscopy and acquisition system, which is applied in the directions of Raman scattering, spectrometry/spectrophotometry/monochromator, optical radiation measurement, etc., can solve problems affecting Raman spectrum acquisition, and achieve elimination of influence, Eliminates the effect of background fluorescence

Inactive Publication Date: 2016-08-17
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The traditional Raman spectrum acquisition system uses a continuous laser for excitation, and the detector also performs continuous integration. It is easy to add the entire fluorescent background to the Raman spectrum, which seriously affects the acquisition of the Raman spectrum.

Method used

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  • Raman spectrum acquisition system with low-fluorescence background
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Embodiment 1

[0031] see figure 2 , an embodiment of the present invention provides a Raman spectrum acquisition system with a low fluorescence background, the Raman spectrum acquisition system includes: a light source, a sample cell, a polarizer, an electro-optic modulator, a gate control circuit, a spectroscopic element, and a detector ,

[0032] Among them, the light source is a pulsed laser, which is used to generate a pulsed excitation beam; the sample cell is used to hold the sample to be tested; the polarizer is used to convert Raman scattered light into linearly polarized light; the electro-optic modulator is used to change the light transmission of the electro-optic crystal axis direction, to limit the passage of linearly polarized light; the gating circuit is used to change the transmission axis direction of the electro-optic crystal in the electro-optic modulator through the change of the output voltage; the light-splitting element uses a grating to split the Raman scattered bea...

Embodiment 2

[0036] Combine below figure 2 1. The specifications and parameters of specific devices introduce the scheme in Embodiment 1 in detail, see the following description for details:

[0037] A picosecond pulsed laser is used as a light source to generate an excitation beam with a center wavelength of 532nm, a power of not less than 50mW and a linewidth of not greater than 0.6nm. The pulsed excitation beam is irradiated vertically to the sample cell, and the sample to be measured is excited to generate a Raman scattering beam. The Raman scattered beam is converted into linearly polarized light by a polarizer.

[0038] Wherein, the electro-optic modulator can be a Kerr modulator or a Pockels modulator, and by changing the direction of the light transmission axis, the passage of linearly polarized light can be restricted.

[0039] The gating circuit changes the direction of the light transmission axis of the electro-optic modulator through the change of the output voltage, thereby...

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Abstract

The invention discloses a Raman spectrum acquisition system with a low-fluorescence background, and relates to the technical field of spectrum analysis instruments. The Raman spectrum acquisition system is based on Raman scattering which is an instantaneous effect, and the duration time of the Raman scattering is much shorter than the fluorescence lifetime; a pulse laser is adopted to carry out excitation; through a polarizer, an electro-optical modulator and a gate control circuit, the acquisition time of the Raman scattering beam is limited; when the optical exciting pulse is finished, acquisition of the Raman scattering light is stopped at the same time. The Raman spectrum acquisition system can be used for enabling the fluorescence signals in a follow-up time not to be detected by a detector, so as to eliminate the influence, caused by the fluorescence background, on a Raman spectrum. The Raman spectrum acquisition system is simple in structure, and can be used for eliminating most of the background fluorescence and effectively reducing the interference, caused by the fluorescence, to the Raman scattering spectrum.

Description

technical field [0001] The invention relates to the technical field of spectrum analysis instruments, in particular to a Raman spectrum acquisition system with a low fluorescence background. Background technique [0002] Raman spectroscopy is a kind of scattering spectrum, which is the scattering of frequency changes caused by the interaction between incident light and molecules when light passes through the medium. It is a spectral analysis method that uses molecular vibration-rotation information. As a newly developed analysis method, it can provide fast, simple, repeatable detection without pretreatment of samples, and can be directly measured by fiber optic probe or quartz vessel, so as to perform non-destructive and rapid qualitative and quantitative analysis of substances. analyze. [0003] Raman spectroscopy is a non-destructive testing technique that is applicable to various physical forms of substances. Its applications include: materials, chemical industry, biomed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01J3/44
CPCG01N21/65G01J3/44
Inventor 李奇峰杜文芳王慧捷马翔云
Owner TIANJIN UNIV