Novel construction method of biodegradable porous PHBV/PPC (poly (3-hydrobutyrate-3-hydroxyvalerate)/polypropylene carbonate) composite scaffold

Abstract

The invention discloses a construction method of a poly (3-hydrobutyrate-3-hydroxyvalerate) / polypropylene carbonate (PHBV-PPC) porous scaffold by virtue of a reactive melt blending-ultrasonic particulate leaching-alkali treatment technology. The construction method comprises the following steps: mixing MA (1-2%), DCP (0.1-0.5%) and the PPC at 130-160 DEG C by 30rpm, so that MA-terminated PPC (MA-PPC) is obtained; mixing the PHBV with GMA (1-15%) and DCP (0.1-0.5%) at 165-175 DEG C, so that GMA-modified PHBV is obtained; and conducting reactive blending and ultrasonic particulate leaching on the MA-PPC, the GMA-modified PHBV and NaCl at 165-175 DEG C, soaking in 1-2mol / L of NaOH for 60-120min, washing in water and freeze-drying. The constructed scaffold, applied to tissue repair, is expected to achieve popularization and application in the fields of bio-medical materials and ecological environmental protection.

Description

technical field

[0001] The invention relates to a method for constructing a fully biodegradable porous poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) / polypropylene carbonate (PPC) composite scaffold, in particular to a reactivity-based The PHBV / PPC composite scaffold method constructed by melt blending-particle ultrasonic leaching-alkali treatment technology belongs to the field of biodegradable materials and is applied in the fields of food packaging, medical equipment, drug sustained release or tissue engineering. Background technique

[0002] Tissue engineering is an in vitro reconstitution technique of living organisms and is an effective strategy to repair or replace damaged or diseased tissues. Proliferating cells quickly and maintaining the bioactivity of active molecules using suitable scaffolds is a great challenge in the field of tissue engineering. Multi-level construction of functional scaffolds that can mediate cell growth, induce cell-specific differentiatio...

Examples

Embodiment 1

[0026]MA, DCP and PPC are mixed at 30rpm in a Haake mixer at 160°C to obtain maleic anhydride-terminated PPC (MA-PPC); PHBV is mixed with GMA (5%) and DCP (0.5%) at 165°C to obtain GMA modified PHBV, MA-PPC (10%), GMA modified PHBV (10%), and porogen NaCl (80%) are reactively melt blended at 165°C, hot pressed into sheets, and cold pressed. The blended product was soaked in distilled water, and the water-soluble components were removed by particle ultrasonic leaching in a 37°C water bath, and the weight of the scaffold was weighed until there was no change to determine the complete dissolution of salt ions. Finally, it was soaked in 2mol / L NaOH solution for 90 minutes, washed with water until neutral, and freeze-dried to obtain a porous PHBV / PPC composite scaffold with a porosity of 71.34%. The liver cancer cells were fixed on the support, and the adhesion and growth state of the cells on the surface of the material was observed with a scanning electron microscope (SEM) (as sh...

Embodiment 2

[0028] MA, DCP and PPC were mixed in a 30rpm, 160°C Haake mixer to obtain MA-capped PPC (MA-PPC); PHBV was mixed with GMA (5%) and DCP (0.5%) at 165°C to obtain GMA modified For PHBV, MA-PPC (3%), PHBV-GMA graft (7%), and porogen NaCl (90%) are reactively melt-blended at 165°C, hot-pressed into tablets, and cold-pressed. The blended product was immersed in distilled water, and the water-soluble components were ultrasonically removed in a 37°C water bath by particle ultrasonic leaching, and the weight of the scaffold was weighed until there was no change to determine the complete dissolution of salt ions. Finally, it was soaked in 2mol / L NaOH solution for 90 minutes, washed with water until neutral, and freeze-dried to obtain a porous PHBV / PPC composite scaffold with a porosity of 82.79%, an appropriate in vitro degradation cycle, and excellent mechanical properties. It can be used as a tissue engineering carrier material. For tissue repair and reconstruction.

Embodiment 3

[0030] MA, DCP and PPC were mixed in a 30rpm, 160°C Haake mixer to obtain MA-capped PPC (MA-PPC); PHBV was mixed with GMA (5%) and DCP (0.5%) at 165°C to obtain GMA modified For PHBV, MA-PPC (7%), PHBV-GMA graft (3%), and porogen NaCl (90%) are reactively melt-blended at 165°C, hot-pressed into tablets, and cold-pressed. The blended product was immersed in distilled water, and the water-soluble components were ultrasonically removed in a 37°C water bath by particle ultrasonic leaching, and the weight of the scaffold was weighed until there was no change to determine the complete dissolution of salt ions. Finally, it was soaked in 2mol / L NaOH solution for 90 minutes, washed with water until neutral, and freeze-dried to obtain a porous PHBV / PPC composite scaffold with a porosity of 82.9%, an appropriate in vitro degradation cycle, and excellent mechanical properties. It can be used as a tissue engineering carrier material. For tissue repair and reconstruction.

technical field

[0001] The invention relates to a method for constructing a fully biodegradable porous poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) / polypropylene carbonate (PPC) composite scaffold, in particular to a reactivity-based The PHBV / PPC composite scaffold method constructed by melt blending-particle ultrasonic leaching-alkali treatment technology belongs to the field of biodegradable materials and is applied in the fields of food packaging, medical equipment, drug sustained release or tissue engineering. Background technique

[0002] Tissue engineering is an in vitro reconstitution technique of living organisms and is an effective strategy to repair or replace damaged or diseased tissues. Proliferating cells quickly and maintaining the bioactivity of active molecules using suitable scaffolds is a great challenge in the field of tissue engineering. Multi-level construction of functional scaffolds that can mediate cell growth, induce cell-specific differentiatio...

Examples

Embodiment 1

[0026]MA, DCP and PPC are mixed at 30rpm in a Haake mixer at 160°C to obtain maleic anhydride-terminated PPC (MA-PPC); PHBV is mixed with GMA (5%) and DCP (0.5%) at 165°C to obtain GMA modified PHBV, MA-PPC (10%), GMA modified PHBV (10%), and porogen NaCl (80%) are reactively melt blended at 165°C, hot pressed into sheets, and cold pressed. The blended product was soaked in distilled water, and the water-soluble components were removed by particle ultrasonic leaching in a 37°C water bath, and the weight of the scaffold was weighed until there was no change to determine the complete dissolution of salt ions. Finally, it was soaked in 2mol / L NaOH solution for 90 minutes, washed with water until neutral, and freeze-dried to obtain a porous PHBV / PPC composite scaffold with a porosity of 71.34%. The liver cancer cells were fixed on the support, and the adhesion and growth state of the cells on the surface of the material was observed with a scanning electron microscope (SEM) (as sh...

Embodiment 2

[0028] MA, DCP and PPC were mixed in a 30rpm, 160°C Haake mixer to obtain MA-capped PPC (MA-PPC); PHBV was mixed with GMA (5%) and DCP (0.5%) at 165°C to obtain GMA modified For PHBV, MA-PPC (3%), PHBV-GMA graft (7%), and porogen NaCl (90%) are reactively melt-blended at 165°C, hot-pressed into tablets, and cold-pressed. The blended product was immersed in distilled water, and the water-soluble components were ultrasonically removed in a 37°C water bath by particle ultrasonic leaching, and the weight of the scaffold was weighed until there was no change to determine the complete dissolution of salt ions. Finally, it was soaked in 2mol / L NaOH solution for 90 minutes, washed with water until neutral, and freeze-dried to obtain a porous PHBV / PPC composite scaffold with a porosity of 82.79%, an appropriate in vitro degradation cycle, and excellent mechanical properties. It can be used as a tissue engineering carrier material. For tissue repair and reconstruction.

Embodiment 3

[0030] MA, DCP and PPC were mixed in a 30rpm, 160°C Haake mixer to obtain MA-capped PPC (MA-PPC); PHBV was mixed with GMA (5%) and DCP (0.5%) at 165°C to obtain GMA modified For PHBV, MA-PPC (7%), PHBV-GMA graft (3%), and porogen NaCl (90%) are reactively melt-blended at 165°C, hot-pressed into tablets, and cold-pressed. The blended product was immersed in distilled water, and the water-soluble components were ultrasonically removed in a 37°C water bath by particle ultrasonic leaching, and the weight of the scaffold was weighed until there was no change to determine the complete dissolution of salt ions. Finally, it was soaked in 2mol / L NaOH solution for 90 minutes, washed with water until neutral, and freeze-dried to obtain a porous PHBV / PPC composite scaffold with a porosity of 82.9%, an appropriate in vitro degradation cycle, and excellent mechanical properties. It can be used as a tissue engineering carrier material. For tissue repair and reconstruction.

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