A kind of Saccharomyces cerevisiae engineering bacteria for producing betulinic acid and construction method thereof

A technology of Saccharomyces cerevisiae and betulinic acid, applied in the field of genetic modification of microorganisms, can solve problems such as waste of resources
CN105886415BActive Publication Date: 2019-07-19湖北济安堂药业股份有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
湖北济安堂药业股份有限公司
Publication Date
2019-07-19

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Abstract

The invention relates to an engineered strain of saccharomyces cerevisiae for producing a betulinic acid. The engineered strain of the saccharomyces cerevisiae is characterized in that an LUP gene expression cassette and a BPLO gene expression cassette are integrated in a genome on the basis of a saccharomyces cerevisiae strain WAT11; and a GAL80 gene is knocked out with priority from the engineered strain of the saccharomyces cerevisiae. The invention further relates to a method for building the engineered strain of the saccharomyces cerevisiae and a method for producing the betulinic acid employing the engineered strain of the saccharomyces cerevisiae. Through the engineered strain of the saccharomyces cerevisiae, the betulinic acid can be produced employing the saccharomyces cerevisiae in a microbial fermentation manner; the operation is simple; the yield is high; the production cycle is short; and the floor area is small.
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Description

technical field

[0001] The invention relates to the genetic modification of microorganisms, more particularly to a Saccharomyces cerevisiae engineering bacterium producing betulinic acid and a construction method thereof. Background technique

[0002] Betulinic acid (BA), also known as betulinic acid, is a pentacyclic triterpenoid compound. Pentacyclic triterpenoids are a very important class of plant secondary metabolites, which usually have a wide range of pharmacological effects and biological activities. This type of compound exists widely in the plant kingdom, and contains a large number of types. The main pentacyclic triterpenes are oleanane type, ursane type, lupinane type and suberane type. Betulinic acid is a typical representative of lupine-type pentacyclic triterpenoids.

[0003] A large number of studies in recent years have shown that betulinic acid has significant pharmacological effects and biological activities, mainly in anti-tumor, anti-HIV, anti-inflamma...

Claims

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