Esophageal cancer markers

A technology for esophageal cancer and expression products, applied in the biological field, can solve problems such as doubts about the curative effect of surgical operations, confidence blows to patients and surgeons, etc.

Active Publication Date: 2019-09-27
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is reported at home and abroad that the early postoperative recurrence and metastasis rate (within 1 year after operation) can reach about 20-50%, which is a heavy blow to the confidence of patients and surgeons, and some patients even have doubts about the curative effect of surgery.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Expression difference of PCP4L1 gene in normal esophageal tissue and esophageal cancer tissue

[0062] 1. Experimental materials:

[0063] Esophageal cancer tissue is the specimen removed by thoracic surgery in the hospital, and normal esophageal tissue is the specimen taken out under the gastroscope. scalpel blade.

[0064] All cases were confirmed pathologically. Including 40 cases of esophageal cancer tissue and 30 cases of normal esophageal tissue. Among them, there were 34 cases of esophageal squamous cell carcinoma and 6 cases of esophageal adenocarcinoma. Normal esophageal tissue was used as the control group. All patients were pathologically confirmed before operation, and no radiotherapy, chemotherapy and other treatments were performed before operation, and no tumors in other parts were found. All tissues were stored in liquid nitrogen within half an hour of the operation, and then transferred to a -80°C refrigerator for freezing.

[0065] 2. E...

Embodiment 2

[0087] Embodiment 2 PCP4L1 gene expression plasmid construction

[0088] 1. Construction of PCP4L1 gene expression vector

[0089] Amplification primers were designed according to the coding sequence of the PCP4L1 gene (as shown in SEQ ID NO.1). Amplify the coding sequence of the full-length PCP4L1 gene from the cDNA library of adult fetal brain (clontech company, article number: 638831), insert the above cDNA sequence into the eukaryotic cell expression vector pcDNA3.1, and connect the obtained recombinant vector pcDNA3.1 -PCP4L1 was used in subsequent experiments.

[0090] 2. Culture and transfection of esophageal cancer cells

[0091] 2.1 Cell culture

[0092] The esophageal cancer cell line ECA109 was inoculated in DMEM medium containing 10% fetal bovine serum, and placed at 37°C, 5% CO 2 Cultivate in an incubator, and when the cells reach 80% confluence, they are digested and passaged with 0.25% trypsin.

[0093] 2.2 Cell transfection

[0094] Esophageal cancer cell...

Embodiment 3

[0118] Example 3 Effect of PCP4L1 gene on the proliferation of esophageal cancer cells

[0119] 1. Cell transfection: Esophageal cancer cells were transfected with pcDNA3.1-PCP4L1 and pcDNA3.1 according to the method in Example 2.

[0120] 2. Add 3H-TdR (1 μCi / well) 24 hours after transfection, culture for another 24 hours, collect cells, add liquid scintillation fluid, and detect the cpm value with a β counter.

[0121] 3. Statistical methods

[0122] The experiments were repeated 3 times, and the results were expressed in the form of mean ± standard deviation. SPSS13.0 statistical software was used for statistical analysis. The difference between the PCP4L1 gene overexpression group and the control group Using t test, it is considered statistically significant when P<0.05.

[0123] 4. Results

[0124] Figure 5 The results shown showed that the growth rate of cells transfected with pcDNA3.1-PCP4L1 group was significantly lower than that of cells transfected with pcDNA3.1...

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Abstract

The invention discloses a molecular marker for diagnosis and treatment of esophagus cancer. A PCP4L1 gene can serve as the molecular marker. Whether a subject suffers from the esophagus cancer or has the esophagus cancer risks or not can be judged through detection of content of the PCP4L1 gene in esophagus tissues. Overexpressed PCP4L1 gene expression capable of inhibiting esophagus cancer cell proliferation is discovered through studies on proliferation conditions of esophagus cancer cells cultured in vitro, and study results show that the PCP4L1 gene is a potential drug target for treating the esophagus cancer.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of PCP4L1 gene in the diagnosis and treatment of esophageal cancer. Background technique [0002] Esophageal cancer (Esophageal carcinoma, EC) is a common malignant tumor of the digestive tract, accounting for the second place in the death of tumors in various parts of the digestive system. The world dies from this disease every year, reaching 300,000 people, of which about 150,000 people die in China every year. It is the country with the highest incidence rate of esophageal cancer in the world. Although great progress has been made in chemotherapy, radiotherapy, and concurrent chemoradiotherapy for esophageal cancer in recent years, surgery is still the most important treatment for esophageal cancer. However, the treatment effect is not ideal, and the 5-year survival rate is only about 30%. The main cause of death is postoperative recurrence and metastasis, especia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/68G01N33/574A61K45/00A61P35/00
Inventor 杨承刚肖枫宋宏涛
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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