Supercharge Your Innovation With Domain-Expert AI Agents!

A method for quantifying catalase

A technology of catalase and hydrogen peroxide, which is applied in the direction of measuring devices, material analysis through optical means, instruments, etc., can solve the problems of complicated operation and poor accuracy, and achieve high sensitivity, convenient operation and wide detection range Effect

Active Publication Date: 2018-05-29
YUNNAN SHENGQING ENVIRONMENTAL MONITORING CO LTD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the problems of poor accuracy and complex operation of conventional detection, a method for rapid and accurate determination of catalase was invented

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] A method for quantifying catalase of the present invention is characterized in that it comprises the following three chemical substances: hydrogen peroxide, phenyl oxalate and 9,10-diphenylanthracene fluorescent dye; Hydrogen reacts with phenyl oxalate, hydrogen peroxide nucleophilic attack on the carbonyl of phenyl oxalate to generate a dioxy ring intermediate dioxetanedione which can generate high energy; the intermediate decomposes the energy Passed to the acceptor fluorescent molecule, so that it is in an excited state; the excited state of 9,10-diphenylanthracene is unstable, decomposes back to the stable ground state, and emits fluorescence, while catalase can catalyze H 2 o 2 Decompose to generate H 2 O and O 2 , which can lead to the weakening or quenching of the fluorescent signal;

[0020] In the range of 0.2-3U / mL, the intensity of the fluorescence signal change is linearly negatively correlated with the content of catalase, showing a change in the charact...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for quantifying catalase, and belongs to the technical field of biological detection. According to the method, the following three kinds of chemical matter of hydrogen peroxide, phenyl oxalate ester and 9,10-diphenyl-anthracene fluorochrome are used; energy generated by the chemical reaction between hydrogen peroxide and phenyl oxalate ester is utilized for motivating 9,10-diphenyl-anthracene to generate fluorescence signals, catalase can restrain the chemical reaction, and consequently the fluorescence signal is weakened or quenched and converted into an electric signal, and the content of catalase is measured through the strength of the electric signal. According to the method, catalase can be quantified fast and accurately, high sensitivity is achieved, operation is convenient, the detecting range is wide, and trace and high-concentration catalase can be precisely quantified.

Description

technical field [0001] The invention relates to a method for quickly measuring the content of catalase, which belongs to the technical field of biological detection. Background technique [0002] In the process of metabolism, organisms will produce some harmful substances. Only when these harmful substances are quickly decomposed and removed, can organisms maintain normal life activities. Hydrogen peroxide is a harmful substance produced by organisms. It has strong oxidizing properties and is very harmful to organisms. It needs to be decomposed quickly. Catalase can catalyze the decomposition of hydrogen peroxide and produce oxygen, which is closely related to biological respiration, photosynthesis and auxin oxidation. During the growth of organisms, the activity of catalase changes continuously, and the concentration of catalase can reflect the changes in the metabolism of organisms in a certain period. For example, in the process of biological treatment of sewage, the ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 宣志刚黄志红王万宾崔文娟周涛顾开林罗生宋羽飞
Owner YUNNAN SHENGQING ENVIRONMENTAL MONITORING CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com