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Preparation method of agricultural liquid microbial agent

A microbial agent and microbial technology, which is applied in the field of preparation of agricultural liquid microbial agent, can solve the problems of inability to exert the effect of microbial agent, death of "indigenous" microorganisms, and inability to form a population effect, so as to improve crop quality and strengthen Survival competitiveness, strong acid resistance effect

Inactive Publication Date: 2016-08-31
纪新强
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the serious soil compaction and acidification, the external temperature is erratic, and the soil environment has its own "indigenous" microorganisms, the microbial agents cultivated in the greenhouse are directly put into the soil, because they cannot adapt to the harsh environment and the competition of "indigenous" microorganisms in the soil. Basically all of them died, even if a small number survived because they could not form a population effect, they would not be able to exert the effect of microbial agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Add kelp, soybean meal, wheat bran, slaked lime and distilled water into the fermenter according to the mass ratio of 200:500:300:1:350, and sterilize at 120°C for 20 minutes to obtain product A; and prepare fermented seed liquid: Subtilis subtilis Bacillus was inoculated into a liquid medium at 35°C and cultured for 26 hours; the liquid medium was: glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.8g, sulfuric acid Zinc 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Bacillus subtilis and liquid medium is 8:100; the seed liquid is added to product A, wherein the quality of the seed liquid and product A The ratio is 8:100, anaerobic fermentation at 35°C, pH 6.5-7.5 for 8 days to obtain product B; adjust the pH of product B to 5.0-5.5, cool the temperature of the fermenter to 4°C, store for 4 days, and The temperature was restored to 35°C to obtain product C; the s...

Embodiment 2

[0038] Add kelp, Enteromorpha, wheat bran, slaked lime and distilled water into the fermenter according to the mass ratio of 200:500:300:1:350 and sterilize at 130°C for 15 minutes to obtain product A; and prepare fermented seed liquid: Brazil Azospirillum and Bacillus subtilis were inoculated into the liquid medium at 35°C and cultured for 26 hours; the liquid medium was: the liquid medium was: glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, Dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.8g, zinc sulfate 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Azospirillum brasiliensis, Bacillus subtilis and liquid medium is 4:4 : 100; seed liquid is added in product A, wherein the mass ratio of seed liquid and product A is 8:100, at 35 ℃, aerobic fermentation 6 days under the condition of pH6.5~7.5, obtain product B; Adjust product B pH to 5.0-5.5, cooling the temperature of the fermenter to 4°C, storing for 3 days, retur...

Embodiment 3

[0040] Add kelp, enteromorpha, wheat bran, slaked lime and distilled water into the fermenter according to the mass ratio of 200:500:300:2:300 and sterilize at 115°C for 25 minutes to obtain product A; and prepare fermented seed liquid: Subtilis subtilis Bacillus was inoculated into the liquid medium at 30°C and cultured for 26 hours; the liquid medium was: the liquid medium was: glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, dipotassium hydrogen phosphate 0.2 g, magnesium sulfate 0.8g, zinc sulfate 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Bacillus subtilis and liquid medium is 8:100;

[0041]Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 8:100, and ferment aerobically at 30°C and pH6.5-7.5 for 5 days to obtain product B; adjust the pH of product B to 5.0 to 5.5, cool the temperature of the fermenter to 5°C, store for 3 days, return the temperature to 30°C, and obtain product ...

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Abstract

The invention relates to a preparation method of an agricultural liquid microbial agent. Plant residues, an acid-base adjusting agent, distilled water, a culture medium and microorganisms are added into a fermentation tank for fermentation under the certain temperature, fermentation is performed for a certain period of time, products are subjected to pH adjustment and cooling and then stored in the fermentation tank for 3-5 d, hydrated lime is added for pH adjustment, the culture medium and beneficial microorganisms are added into the fermentation tank continuously, fermentation is performed under the certain temperature, the fermented products are subjected to pH adjustment and cooling and then stored in the fermentation tank for 3-5 d, the steps are executed again, and a filtrate obtained after filter pressing is performed is the agricultural liquid microbial agent. The method simulates the natural soil ecological environment, the fermented microbial agent is put into soil through three-time fermentation, the agricultural liquid microbial agent can adapt to the environment rapidly, and the survival rate is ensured.

Description

technical field [0001] The invention relates to a preparation method of a liquid microbial bacterial agent for agriculture. Background technique [0002] The evaluation indicators for agricultural microorganisms mainly include the purity of the microbial agent and the quantity of the microbial agent per unit mass. Therefore, the preparation method of bacterial agents as the prior art is generally: a fermentation process is carried out under the temperature and pH conditions suitable for microorganisms to survive, which is equivalent to greenhouse cultivation. [0003] Due to the serious soil compaction and acidification, the external temperature is erratic, and the soil environment has its own "indigenous" microorganisms, the microbial agents cultivated in the greenhouse are directly put into the soil, because they cannot adapt to the harsh environment and the competition of "indigenous" microorganisms in the soil. Basically all die, even if there is a small amount of survi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/14A01N63/00C12N1/20C12N1/16A01P21/00A01P5/00A01P7/04C05F11/08C09K101/00C12R1/41C12R1/07C12R1/125
CPCC09K17/14A01N63/00C05F11/08C09K2101/00C12N1/16C12N1/20
Inventor 姚继胜纪新强
Owner 纪新强
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