Preparation method of agricultural liquid microbial agent
A microbial agent and microbial technology, which is applied in the field of preparation of agricultural liquid microbial agent, can solve the problems of inability to exert the effect of microbial agent, death of "indigenous" microorganisms, and inability to form a population effect, so as to improve crop quality and strengthen Survival competitiveness, strong acid resistance effect
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Embodiment 1
[0036] Add kelp, soybean meal, wheat bran, slaked lime and distilled water into the fermenter according to the mass ratio of 200:500:300:1:350, and sterilize at 120°C for 20 minutes to obtain product A; and prepare fermented seed liquid: Subtilis subtilis Bacillus was inoculated into a liquid medium at 35°C and cultured for 26 hours; the liquid medium was: glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.8g, sulfuric acid Zinc 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Bacillus subtilis and liquid medium is 8:100; the seed liquid is added to product A, wherein the quality of the seed liquid and product A The ratio is 8:100, anaerobic fermentation at 35°C, pH 6.5-7.5 for 8 days to obtain product B; adjust the pH of product B to 5.0-5.5, cool the temperature of the fermenter to 4°C, store for 4 days, and The temperature was restored to 35°C to obtain product C; the s...
Embodiment 2
[0038] Add kelp, Enteromorpha, wheat bran, slaked lime and distilled water into the fermenter according to the mass ratio of 200:500:300:1:350 and sterilize at 130°C for 15 minutes to obtain product A; and prepare fermented seed liquid: Brazil Azospirillum and Bacillus subtilis were inoculated into the liquid medium at 35°C and cultured for 26 hours; the liquid medium was: the liquid medium was: glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, Dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.8g, zinc sulfate 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Azospirillum brasiliensis, Bacillus subtilis and liquid medium is 4:4 : 100; seed liquid is added in product A, wherein the mass ratio of seed liquid and product A is 8:100, at 35 ℃, aerobic fermentation 6 days under the condition of pH6.5~7.5, obtain product B; Adjust product B pH to 5.0-5.5, cooling the temperature of the fermenter to 4°C, storing for 3 days, retur...
Embodiment 3
[0040] Add kelp, enteromorpha, wheat bran, slaked lime and distilled water into the fermenter according to the mass ratio of 200:500:300:2:300 and sterilize at 115°C for 25 minutes to obtain product A; and prepare fermented seed liquid: Subtilis subtilis Bacillus was inoculated into the liquid medium at 30°C and cultured for 26 hours; the liquid medium was: the liquid medium was: glucose 12g, peptone 4g, soluble starch 2g, potassium nitrate 0.6g, dipotassium hydrogen phosphate 0.2 g, magnesium sulfate 0.8g, zinc sulfate 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Bacillus subtilis and liquid medium is 8:100;
[0041]Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 8:100, and ferment aerobically at 30°C and pH6.5-7.5 for 5 days to obtain product B; adjust the pH of product B to 5.0 to 5.5, cool the temperature of the fermenter to 5°C, store for 3 days, return the temperature to 30°C, and obtain product ...
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