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Antibacterial gel covalently grafted antibacterial polypeptide and preparation method thereof

A technology of antibacterial polypeptide and covalent grafting, applied in bandages, absorbent pads, medical science, etc., can solve the problems of loss of antibacterial ability, high localization, drug burst release, etc., and achieve good antibacterial ability, high selectivity, and responsiveness The effect of mild conditions

Active Publication Date: 2019-04-09
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Current hydrogel dressings typically entrap antimicrobials and drugs physically, which may lead to problems with drug burst release
For example, hydrogels loaded with silver ions are prone to local overheating due to the sudden release of silver ions in the early stage of use, resulting in certain toxicity.
As the drug continues to be released, eventually the gel also loses its antimicrobial ability

Method used

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  • Antibacterial gel covalently grafted antibacterial polypeptide and preparation method thereof
  • Antibacterial gel covalently grafted antibacterial polypeptide and preparation method thereof
  • Antibacterial gel covalently grafted antibacterial polypeptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Dissolve 0.5g of sodium alginate in 100ml of deionized water, add 0.750g of 4-(4,6-dimethoxytriazin-2-yl)-4-methylmorpholine hydrochloride, and stir for 0.5h , then added 490 μl of furfurylamine dropwise, and reacted in the dark for 24 hours. Dialysis was performed for 5 days using a dialysis bag with a molecular weight cut-off of 3500. Freeze-dry at -80°C to obtain a solid modified sodium alginate.

[0032] (2) Dissolve 75mg of modified sodium alginate in 5ml of deionized water, stir for 2 hours, add 50mg of bismaleimide-terminated polyvinyl alcohol with a molecular weight of 2000 after it is completely dissolved, stir for 10 minutes to dissolve, and then ultrasonicate for 5 minutes Transfer to a cylindrical mold with a height of 5mm and a diameter of 10mm, and react and cross-link at a temperature of 37°C to obtain a gel.

[0033] (3) Soak the above-mentioned gel in 1ml of CYS-HHC-10 antibacterial polypeptide solution with a concentration of 1mg / mL to fully swel...

Embodiment 2

[0035] (1) Dissolve 0.5g of sodium alginate in 100ml of deionized water, add 0.750g of 4-(4,6-dimethoxytriazin-2-yl)-4-methylmorpholine hydrochloride, and stir for 0.5h , then added 490 μl of furfurylamine dropwise, and reacted in the dark for 24 hours. Dialysis was performed for 5 days using a dialysis bag with a molecular weight cut-off of 3500. Freeze-dry at -80°C to obtain a solid modified sodium alginate.

[0036] (2) Dissolve 75mg of modified sodium alginate in 5ml of deionized water, stir for 2 hours, add 50mg of bismaleimide-terminated polyvinyl alcohol with a molecular weight of 2000 after it is completely dissolved, stir for 10 minutes to dissolve, and then ultrasonicate for 5 minutes Transfer to a cylindrical mold with a height of 5mm and a diameter of 10mm, and react and cross-link at a temperature of 37°C to obtain a gel.

[0037] (3) Soak the above gel in 1ml of CYS-HHC-10 antibacterial polypeptide solution with a concentration of 2mg / mL, and fully swell. Then...

Embodiment 3

[0039] (1) Dissolve 0.5g of sodium alginate in 100ml of deionized water, add 0.750g of 4-(4,6-dimethoxytriazin-2-yl)-4-methylmorpholine hydrochloride, and stir for 0.5h , then added 490 μl of furfurylamine dropwise, and reacted in the dark for 24 hours. Dialysis was performed for 5 days using a dialysis bag with a molecular weight cut-off of 3500. Freeze-dry at -80°C to obtain a solid modified sodium alginate.

[0040] (2) Dissolve 75mg of modified sodium alginate in 5ml of deionized water, stir for 2 hours, add 50mg of bismaleimide-terminated polyvinyl alcohol with a molecular weight of 2000 after it is completely dissolved, stir for 10 minutes to dissolve, and then ultrasonicate for 5 minutes Transfer to a cylindrical mold with a height of 5mm and a diameter of 10mm, and react and cross-link at a temperature of 37°C to obtain a gel.

[0041] (3) Soak the above-mentioned gel in 1 ml of CYS-HHC-10 antibacterial polypeptide solution with a concentration of 3 mg / mL, and fully ...

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Abstract

The invention belongs to the technical field of biomedical materials, and discloses antibacterial gel in covalent grafting with antibacterial polypeptide and a preparation method of the antibacterial gel. The preparation method comprises the following steps: adding an activating agent into water solution of sodium alginate for activation, after activation, adding with furfuryl amine for light-tight reaction, and carrying out dialysis and freeze-drying to obtain modified sodium alginate solid; dissolving the modified sodium alginate solid into deionized water, adding with maleimide-terminated polyving akohol, and carrying out crosslinking reaction under 37-60 DEG C for obtaining gel; and soaking the obtained gel by antibacterial polypeptide solution, and grafting antibacterial polypeptide into the gel under the ultraviolet light condition by adopting a photoinitiator, thus obtaining a product. The antibacterial gel prepared by adopting the method provided by the invention has excellent antibacterial ability for escherichia coli, and can promote the adhesion of human fibroblasts.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an antibacterial gel covalently grafted with antibacterial polypeptide and a preparation method thereof. Background technique [0002] With the development of modern medicine, the wound dressings used in wound care have undergone revolutionary changes. Wound dressings have developed from traditional gauze, bandages, etc. to the current new bioactive dressings. An ideal bioactive dressing requires good biocompatibility, moisture retention and can provide a moist environment for the wound, antibacterial ability and certain biological activity. [0003] As one of the new bioactive dressings, hydrogel dressing can maintain a moist environment for the wound, accelerate wound healing, and can isolate the outside world and prevent bacterial infection of the wound. It has a certain water absorption capacity and can well carry drugs, biological active factors, e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32A61L15/28A61L15/24A61L15/44A61L15/42C08G81/02C08B37/04
CPCA61L15/24A61L15/28A61L15/32A61L15/42A61L15/44A61L2300/252A61L2300/404C08B37/0084C08G81/02C08L5/04C08L29/04
Inventor 曹晓东朱杰华吴水平游柏浩
Owner SOUTH CHINA UNIV OF TECH
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