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Double-crosslinked sodium alginate hydrogel and preparation method and application thereof

A technology of sodium alginate and double cross-linking, which is applied in the fields of medical science and prostheses, and can solve the problems of cumbersome preparation steps of double-network hydrogels, so as to ensure cell compatibility, improve anti-swelling performance, and mild reaction conditions Effect

Active Publication Date: 2016-06-29
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation steps of double-network hydrogels are cumbersome, require free radical polymerization, and microsphere cross-linked hydrogels have special equipment requirements, which limit their application in the field of tissue engineering.

Method used

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  • Double-crosslinked sodium alginate hydrogel and preparation method and application thereof
  • Double-crosslinked sodium alginate hydrogel and preparation method and application thereof
  • Double-crosslinked sodium alginate hydrogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Dissolve 0.5g of sodium alginate in 100mL of deionized water, add 0.495g of 4-(4,6-dimethoxytriazin-2-yl)-4-methylmorpholine hydrochloride, and stir for 0.5h , then added 343 μL of furfurylamine dropwise, and reacted in the dark for 24 hours. Dialysis was performed for 5 days using a dialysis bag with a molecular weight cut-off of 3500. Freeze-dry at -80°C to obtain a furan-modified sodium alginate solid.

[0032] (2) Dissolve 75 mg of modified sodium alginate in 5 mL of deionized water, stir for 2 h, and add 37 mg of bismaleimide-terminated polyvinyl alcohol with a molecular weight of 2000 (purchased from Suzhou Derivative Biotechnology Co., Ltd. , References: Biomacromolecules2011, 12, 824–830), stirred for 10 minutes to dissolve, ultrasonicated for 5 minutes and transferred to a cylindrical mold with a height of 5 mm and a diameter of 10 mm; then the mold was placed in a 40°C oven for 6 hours to obtain a transparent gel.

[0033] (3) The above-mentioned gel was...

Embodiment 2

[0035] (1) Dissolve 0.5g of sodium alginate in 100mL of deionized water, add 0.750g of 4-(4,6-dimethoxytriazin-2-yl)-4-methylmorpholine hydrochloride, and stir for 0.5h , and then added 490 μL of furfurylamine dropwise, and reacted in the dark for 24 hours. Dialysis was performed for 5 days using a dialysis bag with a molecular weight cut-off of 3500. Freeze-dry at -80°C to obtain a furan-modified sodium alginate solid.

[0036] (2) Dissolve 75mg of modified sodium alginate in 5mL of deionized water, stir for 2 hours, add 50mg of bismaleimide terminal polyvinyl alcohol with a molecular weight of 2000 after it is completely dissolved, stir for 10 minutes to dissolve, and then ultrasonicate for 5 minutes Transfer to a cylindrical mold with a height of 5 mm and a diameter of 10 mm; then place the mold in an oven at 40° C. for 6 hours to obtain a transparent gel.

[0037] (3) The above-mentioned gel was soaked in 10 ml of 100 mmol / L calcium chloride solution, and placed in a sha...

Embodiment 3

[0041] (1) Dissolve 0.5g of sodium alginate in 100mL of deionized water, add 1.125g of 4-(4,6-dimethoxytriazin-2-yl)-4-methylmorpholine hydrochloride, and stir for 0.5h , and then added 735 μL of furfurylamine dropwise, and reacted in the dark for 24 hours. Dialysis was performed for 5 days using a dialysis bag with a molecular weight cut-off of 3500. Freeze-dry at -80°C to obtain a furan-modified sodium alginate solid.

[0042] (2) Dissolve 75mg of modified sodium alginate in 5mL of deionized water, stir for 2 hours, add 75mg of bismaleimide terminal polyvinyl alcohol with a molecular weight of 2000 after it is completely dissolved, stir for 10 minutes to dissolve, and then ultrasonicate for 5 minutes Transfer to a cylindrical mold with a height of 5 mm and a diameter of 10 mm; then place the mold in an oven at 40° C. for 6 hours to obtain a transparent gel.

[0043] (3) The above-mentioned gel was soaked in 10 ml of 100 mmol / L calcium chloride solution, and placed in a sha...

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Abstract

The invention belongs to the technical field of biomedical materials and discloses double-crosslinked sodium alginate hydrogel and a preparation method and application thereof. The preparation method includes: dissolving sodium alginate into deionized water, adding activating agents to activate, adding furfurylamine to react for 12-36 hours, and subjecting a reaction product to dialysis and freeze drying so as to obtain furan-modified sodium alginate; dissolving the furan-modified sodium alginate into the deionized water, adding maleimide-terminated polyvinyl alcohol, stirring sufficiently to dissolve, performing ultrasonic treatment, injecting into a mould, and crosslinking at 37-60 DEG C so as to obtain gel; soaking the gel into a calcium chloride solution for 12-48 hours for physical crosslinking so as to obtain the double-crosslinked sodium alginate hydrogel. By the aid of a double-crosslinking method consisting of click chemistry crosslinking and ionic crosslinking, the obtained double-crosslinked sodium alginate hydrogel has excellent mechanical and anti-swelling properties and can be applied to scaffolds for tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a sodium alginate double-crosslinked hydrogel and a preparation method and application thereof. Background technique [0002] Hydrogel is a system composed of a three-dimensional polymer network composed of hydrophilic polymers and water as a medium. Hydrogel can absorb a large amount of water, making it soft and elastic, and water-soluble small molecules can freely diffuse in the three-dimensional network. Its structural properties are very similar to the soft tissue of the organism, and it provides a three-dimensional culture scaffold model for soft tissue repair, which is widely used in tissue engineering. [0003] Sodium alginate is a natural linear polymer polysaccharide. As an excellent material for the preparation of tissue engineering gel scaffolds, it has the advantages of abundant sources, low cost and easy availability, and no immunogenicity. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04C08J3/075A61L27/20A61L27/52
CPCA61L27/20A61L27/52C08B37/0084C08J3/075C08J2305/04C08L5/04
Inventor 曹晓东朱杰华游柏浩吴水平
Owner SOUTH CHINA UNIV OF TECH
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