Efficient detection method for illegally added medicines in bean sprouts
A technology of illegal addition and detection method, applied in the field of detection, can solve the problems of operator injury, cumbersome operation, labor-intensive and other problems, and achieve the effect of improving work efficiency, good repeatability and high degree of automation
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[0025] Embodiment: a kind of detection method of illegally added medicine in bean sprouts, comprises the steps:
[0026] (1) Preparation of standard solution: prepare the standard solution of illegally added drugs with methanol into a standard solution with a concentration of 200 mg / L, store at 4°C; then dilute step by step with methanol to obtain a standard solution with a concentration of 1 mg / L;
[0027] (2) Treatment of the sample to be tested: chop the bean sprouts sample in advance, stir well, weigh 1g into a 50mL centrifuge tube, add 0.1% glacial acetic acid-acetonitrile solution, vortex, place it in an ultrasonic instrument for 20min; centrifuge at 8000rpm for 3min, take Dry the supernatant with nitrogen, then dissolve it with a mixed solution of methanol and water and dilute to 1 mL, wherein the volume ratio of methanol to water is 3:7; filter it into a sample injection vial;
[0028] (3) On-line solid-phase extraction: use HLB extraction column, activate with 1mL met...
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