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Efficient detection method for illegally added medicines in bean sprouts

A technology of illegal addition and detection method, applied in the field of detection, can solve the problems of operator injury, cumbersome operation, labor-intensive and other problems, and achieve the effect of improving work efficiency, good repeatability and high degree of automation

Active Publication Date: 2016-09-07
ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the pretreatments for the determination of illegally added drugs in bean sprouts are organic solvent extraction, which uses a large amount of organic solvents, which is cumbersome to operate and causes harm to operators; or uses solid phase extraction, which is manually operated and consumes a lot of manpower.

Method used

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  • Efficient detection method for illegally added medicines in bean sprouts
  • Efficient detection method for illegally added medicines in bean sprouts
  • Efficient detection method for illegally added medicines in bean sprouts

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Embodiment

[0025] Embodiment: a kind of detection method of illegally added medicine in bean sprouts, comprises the steps:

[0026] (1) Preparation of standard solution: prepare the standard solution of illegally added drugs with methanol into a standard solution with a concentration of 200 mg / L, store at 4°C; then dilute step by step with methanol to obtain a standard solution with a concentration of 1 mg / L;

[0027] (2) Treatment of the sample to be tested: chop the bean sprouts sample in advance, stir well, weigh 1g into a 50mL centrifuge tube, add 0.1% glacial acetic acid-acetonitrile solution, vortex, place it in an ultrasonic instrument for 20min; centrifuge at 8000rpm for 3min, take Dry the supernatant with nitrogen, then dissolve it with a mixed solution of methanol and water and dilute to 1 mL, wherein the volume ratio of methanol to water is 3:7; filter it into a sample injection vial;

[0028] (3) On-line solid-phase extraction: use HLB extraction column, activate with 1mL met...

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Abstract

The invention discloses an efficient detection method for illegally added medicines in bean sprouts. The efficient detection method comprises the following steps: (1) preparation of a standard solution; (2) processing of a to-be-tested sample; (3) online solid-phase extraction; and (4) high performance liquid chromatography-static electric field orbitrap high resolution mass spectrum determination. According to the efficient detection method, an efficient and high-repeatability online solid-phase extraction method is combined with high performance liquid chromatography cascaded static electric field orbitrap high resolution mass spectrum to determine the illegally added medicines in the bean sprouts, so that the qualitative and quantitative determination of the illegally added medicines in the sample can be realized, and the detection result is sensitive and reliable. By virtue of the method, the pretreatment process of the sample is rapid, simple and convenient, so that the working efficiency is improved; by selecting a proper extracting solvent, a cleaning agent, an eluent and proper mass spectrometric detection parameters according to the properties of the illegally added medicines in the bean sprouts, the detection accuracy and reliability are improved. The method is high in automation degree, so that errors caused by manual operation can be effectively avoided.

Description

technical field [0001] The invention belongs to the technical field of detection, and relates to a detection method for illegally added drugs in bean sprouts, in particular to an online solid-phase extraction-high performance liquid chromatography series electrostatic field orbitrap high-resolution mass spectrometry method for illegally added drugs in bean sprouts. Background technique [0002] Illegally added drugs in sprouts include: 4-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid, gibberellins, naphthaleneacetic acid, indolebutyric acid, 6-benzyladenine, carbendazim, and indole Acetic acid etc. Among the above drugs, except that carbendazim belongs to pesticides, other drugs belong to plant growth regulators. This is a class of drugs used to regulate the growth and development of plants, which can promote plant growth, improve the quality and yield of crops. [0003] 4-Chlorophenoxyacetic acid is an organic acid, also known as anti-falling element, which prev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06
CPCG01N30/06G01N2030/062
Inventor 柳菡张睿赵增运沈伟健丁涛吴斌沈崇钰
Owner ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU
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