Construction method and application of a mouse model of HBV infection
A mouse model and construction method technology, applied in the field of medicine, can solve the problems of inability to produce cccDNA and limited duration, and achieve the effect of simple preparation method and simple establishment method
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[0022] 1. Materials
[0023] 1. Cells and plasmids
[0024] Human liver cancer cells Huh7, HepG2, and human embryonic kidney (HEK) 293T cell lines were preserved by the Department of Microbiology, Second Military Medical University (or directly purchased from the Institute of Cells, Chinese Academy of Sciences); the pMD-18T cloning vector and plasmid pUC18 are Takara products.
[0025] 2. Plasmid
[0026] The adeno-associated virus backbone plasmid pAAV-HBV1.2 containing 1.2 copies of the HBV genome was donated by Professor Chen Peizhe of National Taiwan University (it can also be prepared according to the following literature methods: Huang LR, Wu HL, Chen PJ, Chen DS. Animmunocompetent mouse model for the tolerance of human chronic hepatitis Bvirus infection.Proc Natl Acad Sci US A.2006; 103(47):17862-7.); gene cloning vector pMD-18T is a product of Takara Company.
[0027] 3. Primers
[0028] The primers for amplifying the complete HBV genome were synthesized by Takara C...
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