Method for extracting melanin from Stizolobium capitatum seed coats

An extraction method and technology of melanin, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of inability to extract melanin, achieve the effects of short extraction cycle, increased economic value, and simple operation

Inactive Publication Date: 2016-09-21
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the research and development of melanin in pea seed coats in my country, and the current commonly used purification process cannot effectively extract melanin from pea bean seed coats

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The extraction method of Li bean seed coat melanin provided by the invention is as follows:

[0047] Extraction process: After removing impurities by boiling the seed coat of Li beans, add 0.5mol / L NaOH solution according to the ratio of solid to liquid of 1:7.5g / ml, extract at 60°C for 12h, then add 2mol / L NaOH solution The pH of the HCl solution was adjusted to 2, and the colloidal precipitate was collected by centrifugation.

[0048] Purification process: Suspend the collected colloidal precipitate in 15 times the volume of 7mol / L HCl solution, hydrolyze it in a boiling water bath for 2 hours, and after cooling, centrifuge at 5000r / min for 10min to collect solid particles.

[0049] Mix ethanol, ethyl acetate, and acetone with volumes 50 times the mass of the solid particles in turn, shake for 30 minutes, and then centrifuge.

[0050] Dissolve the washed solid particles with 0.5 mol / L NaOH for 30 min under the assistance of ultrasonic waves, centrifuge to remove the ...

Embodiment 2

[0054] The extraction method of Li bean seed coat melanin provided by the invention is as follows:

[0055] Extraction process: After removing impurities by boiling the seed coats of Li beans, add 0.25mol / L NaOH solution according to the ratio of solid to liquid of 1:6g / ml, extract at 50°C for 10h, and then add 2 mol / L HCl The pH of the solution was adjusted to 1, and the colloidal precipitate was collected by centrifugation.

[0056] Purification process: suspend the collected colloidal precipitate in 20 times the volume of 7mol / L HCl solution, hydrolyze it in a boiling water bath for 4 hours, and after cooling, centrifuge at 5000r / min for 10min to collect solid particles.

[0057] Mix ethanol, ethyl acetate, and acetone with volumes 50 times the mass of the solid particles in turn, shake for 25 minutes, and then centrifuge.

[0058] Dissolve the washed solid particles with 0.5 mol / L NaOH for 30 min under the assistance of ultrasonic waves, centrifuge to remove the undissolv...

Embodiment 3

[0062] The extraction method of Li bean seed coat melanin provided by the invention is as follows:

[0063] Extraction process: After removing impurities by boiling the seed coats of Li beans, add 1mol / L NaOH solution according to the ratio of solid to liquid of 1:9g / ml, extract at 70°C for 14h, and then add 2mol / L HCl solution Adjust the pH to 2, and collect by centrifugation to obtain a colloidal precipitate.

[0064] Purification process: Suspend the collected colloidal precipitate in 25 times the volume of 7mol / L HCl solution, hydrolyze it in a boiling water bath for 6 hours, and after cooling, centrifuge at 5000r / min for 10 minutes to collect solid particles.

[0065] Mix ethanol, ethyl acetate, and acetone with volumes 50 times the mass of the solid particles in turn, shake for 35 minutes, and then centrifuge.

[0066] Dissolve the washed solid particles with 0.5 mol / L NaOH for 30 min under the assistance of ultrasonic waves, centrifuge to remove the undissolved part, t...

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PUM

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Abstract

The invention provides a method for extracting melanin from Stizolobium capitatum seed coats. The method comprises the steps: carrying out an extractive process: subjecting the Stizolobium capitatum seed coats to water cooking so as to remove impurities, then, adding the Stizolobium capitatum seed coats into a NaOH solution in accordance with that the material/liquid ratio is 1g: (6 to 9)ml, carrying out extraction for 10 to 14 hours in a nitrogen gas environment, then, adding a HCl solution into the solution so as to adjust the pH to be smaller than 2.5, and carrying out centrifugal collecting, so as to obtain gelatinous precipitates; carrying out a purifying process: suspending the collected gelatinous precipitates in a HCl solution, of which the volume is 15 to 25 times that of the gelatinous precipitates, carrying out hydrolysis in boiling water bath for 2 to 6 hours, carrying out cooling, and then, carrying out centrifugal collecting, so as to obtain solid granules; washing the collected solid granules with ethanol, ethyl acetate and acetone sequentially, and then, carrying out repeated precipitation by using the NaOH solution and the HCL solution, thereby obtaining melanin granules. According to the method, the operation is simple, the ratio of extraction of the melanin from the Stizolobium capitatum seed coats is high, the purity of the melanin is high, the degree of oxidation of the melanin is low, and the extraction cycle is short; in addition, the cost is low, so that the method is suitable for being used for carrying out large-scale extraction on the melanin of the Stizolobium capitatum seed coats; the economic value of Stizolobium capitatum is increased, and the field of application of the Stizolobium capitatum is widened.

Description

technical field [0001] The invention relates to the technical field of extraction technology, in particular to a method for extracting melanin from bean seed coats. Background technique [0002] Melanin is a general term for brown-black to black insoluble pigments widely present in animals, plants and microorganisms, and has important physiological characteristics. In animals and plants, melanin has the function of defense and protection. Existing data indicate that melanin is generally of two types, namely indole type (Eumelanin) and catechol type (Pheomelanin). It is believed that animal melanin mostly belongs to indole type (Eumelanin), while plant source melanin mostly belongs to catechol type (Pheomelanin). [0003] Melanin can provide structural strength to organisms or protect organisms from environmental stresses such as ultraviolet rays, ionizing radiation, heavy metal pollution, low temperature and high temperature. It is not only a kind of photoprotectant, anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 董华强麦钊键
Owner FOSHAN UNIVERSITY
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