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Application of CRK5 protein and coding gene thereof in regulation of plant drought resistance

A technology encoding gene and drought resistance, which is applied to the application field of CRK5 protein and its encoding gene in regulating plant drought resistance, and can solve the problem of unknown specific function of the structural domain and the like.

Active Publication Date: 2016-09-21
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to the typical structural features of RLKs, the extracellular domain of CRKs contains two copies of DUF26 domains (domain of unknown function 26), and each DUF26 domain contains three conserved C-8X-C-2X -C domain, but the specific function of these domains is unknown, it may be related to the interaction between proteins

Method used

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  • Application of CRK5 protein and coding gene thereof in regulation of plant drought resistance
  • Application of CRK5 protein and coding gene thereof in regulation of plant drought resistance
  • Application of CRK5 protein and coding gene thereof in regulation of plant drought resistance

Examples

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Effect test

Embodiment 1

[0047] Embodiment 1, the acquisition and identification of CRK5 transgenic plants

[0048] The CRK5 gene involved in this example is derived from Arabidopsis thaliana, and its sequence in the Arabidopsis genome is shown in sequence 1 in the sequence table, and sequence 1 consists of 2537 nucleotides, which is the CRK5 gene sequence in the Arabidopsis genome, wherein the 818-892, 1034-1103, 1226-1314, 1526-1599, 1838-1909 and 2064-2138 positions are all intron sequences; the cDNA sequence of the CRK5 gene As shown in sequence 2 in the sequence listing, sequence 2 consists of 2082 nucleotides and is the cDNA sequence of the CRK5 gene, wherein the 1-1992nd is the coding sequence (ORF); both sequence 1 and sequence 2 encode the sequence listing The protein shown in sequence 3, sequence 3 consists of 663 amino acid residues.

[0049] 1. Construction of recombinant expression vector pCAMBIA-1300-221-CRK5

[0050] The total RNA of Arabidopsis wild-type (Col ecotype) was extracted, ...

Embodiment 2

[0086] Embodiment 2, CRK5 transgenic plant drought resistance analysis test

[0087] ABA is an important signaling molecule for plants to resist external adversity stress. Under drought conditions, ABA can promote stomatal closure to reduce water loss by regulating ion channels in stomatal guard cells. Calcium ions, protein kinases, and phosphatases are all involved in ABA-mediated guard cell signal transduction and the signal regulation of ABA-related stress resistance genes. Therefore, ABA-induced stomatal movement experiments and drought experiments were used to detect whether CRK5 plays a role in the process of ABA regulating plant drought stress response.

[0088] 1. ABA inhibits stomatal opening and ABA promotes stomatal closing experiment

[0089] (1) ABA inhibits stomatal opening experiment

[0090] With Arabidopsis wild type (Col-0 ecotype), two T that embodiment 1 obtains 3 The homozygous CRK5 transgenic lines OE-1 and OE-2 were used as experimental materials. T...

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Abstract

The invention discloses application of CRK5 protein and a coding gene thereof in regulation of plant drought resistance. The application is particularly the application of the protein (CRK5 protein) composed by an amino acid sequence as shown in the third sequence in a sequence table in a1) improving plant drought resistance and a2) breeding plant varieties of which the drought resistance is improved. Compared with wild type control plants, tolerance of CRK5 gene over-expressed plants to drought adversity stress is significantly improved. The application of the CRK5 protein and the coding gene thereof in regulation of the plant drought resistance has an important meaning on research of a plant drought resistance molecular mechanism; in addition, the gene has an important function in cultivating drought tolerant plant varieties, therefore, an important possibility is provided for cultivating novel adversity-resistant crop varieties, and great significance on agricultural production is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a CRK5 protein and its coding gene in regulating the drought resistance of plants. Background technique [0002] Drought is one of the major natural disasters faced by human beings. Even in today's advanced science and technology, the loss of food production and economic losses caused by it still abound. Today, when the global arable land area is gradually decreasing and drought disasters are occurring more and more frequently, it is of great significance to increase or maintain food production by improving the drought resistance of crops. The traditional breeding technology has the disadvantages of long period, high blindness and heavy workload, and the improvement of grain production through traditional breeding has reached a certain bottleneck. In recent years, with the development of plant molecular biology and genetics and the in-depth study of the molecular me...

Claims

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Application Information

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IPC IPC(8): C12N9/12C12N15/54C12N15/82A01H5/00
CPCC12N9/1205C12N15/8273
Inventor 张大鹏路凯王小芳
Owner TSINGHUA UNIV