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Application of Bacillus jc65 and its volatile substances in plant growth promotion

A volatile substance, JC65 technology, used in plant growth regulators, plant growth regulators, applications, etc., can solve problems such as undisclosed growth-promoting functions, and achieve the goal of promoting sustainable agricultural development, promoting plant growth, and protecting the environment. Effect

Active Publication Date: 2019-05-14
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not disclosed that the bacteria have the function of promoting growth

Method used

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  • Application of Bacillus jc65 and its volatile substances in plant growth promotion
  • Application of Bacillus jc65 and its volatile substances in plant growth promotion
  • Application of Bacillus jc65 and its volatile substances in plant growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 volatile substance has the screening of growth-promoting effect bacterial strain

[0022] Method: The strains with growth-promoting effects of volatile substances were screened by means of separate culture dishes. The seeds of Arabidopsis thaliana were sterilized with 3% NaClO solution for 10 min, ddH 2 O wash 6 times. Spread the prepared Arabidopsis seeds on MS petri dishes and place them in a closed-light incubator at 4°C for low-temperature vernalization for 2 days, then place them in a light incubator for culture (12h light, 20°C, 50% relative humidity, 40W Fluorescent lamps) 2d. Then, the 2d-sized seedlings were spread on one side of the two-partition culture dish containing MS medium, and 10 μL of bacterial solution (concentration of 10 7 CFU / mL), the petri dish was sealed with parafilm, and continued to be placed in the above-mentioned light incubator for cultivation, with 3 seedlings per petri dish, and 6 replicates for each treatment. No bacter...

Embodiment 2

[0029] The preparation method of microbial bacterial agent JC65 bacterial agent is as follows:

[0030] Bacillus JC65 was shaken and cultivated in LB culture medium at 28°C and 180rpm for 12-16h, and then centrifuged at 6000rpm for 10min to obtain the bacterial cells, which were diluted with sterilized water to make bacterial preparations, and the total concentration of viable bacteria in the finished bacterial preparations was 1× 10 9 -1×10 10 CFU / mL.

Embodiment 3

[0031] The growth-promoting test of the volatile substances produced by the microbial bacterial agent JC65 of embodiment 3 on solanaceous vegetables

[0032] Method: After the tomato was sterilized by 3% NaClO solution for 5 minutes, and then germinated on MS medium, the seedlings with consistent germination were transferred to tissue culture bottles, and one seedling was placed in each bottle. In addition, put the sterilized 10mL small beaker containing MS medium in the tissue culture bottle, and inoculate the JC65 bacterial solution (concentration: 5.0×10 7 CFU / mL) 10 μL. The tissue culture bottle was cultured in a light incubator (conditions: light time 12 hours, temperature 28° C., relative humidity 50%). And Escherichia coli (Escherichiacoli) DH5α was used as the blank control group CK, and 24 seedlings were treated for each treatment, with 3 repetitions.

[0033] After 28 days, the plant height, stem diameter, leaf number and fresh weight of tomato were counted.

[00...

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Abstract

The invention discloses application of bacillus JC65 and a volatile substance thereof to plant growth promotion. An existing bacterial strain is developed again and screened, the bacillus JC65 is obtained, the volatile substance generated by the bacillus JC65 can efficiently promote growth of plants in a broad spectrum mode and especially can promote growth of crops such as vegetables and grain and oil crops, the growth promotion effect exceeds 21.55% and can reach 829.14% at the highest level, and quality of the crops is improved. The problem that chemical fertilizer or other methods are poor in effect or residues exist is solved, the environment is protected, sustainable development of agriculture is promoted, and the bacillus JC65 and the volatile substance thereof have good application prospect.

Description

technical field [0001] The invention belongs to the field of plant protection, and relates to the application of bacillus JC65 and its volatile substances in plant growth promotion Background technique [0002] With the development of modern agriculture, the large-scale excessive application of chemical fertilizers has caused great harm to the environment and human health, and has become an important factor affecting the environment, human health and social development. Excessive application of chemical fertilizers will cause deterioration of soil physical and chemical properties, imbalance of soil nutrient structure, and decline of fertility. It also causes soil acidification and compaction, which eventually leads to the degradation of cultivated land soil. And the acidification of the soil will increase the activity of harmful heavy metal elements such as mercury and lead in the soil, resulting in heavy metal pollution and affecting the growth of crops. [0003] Excessiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01N63/00A01P21/00
CPCA01N63/00A01N63/10A01N2300/00
Inventor 郭坚华谢越盛蒋春号
Owner NANJING AGRICULTURAL UNIVERSITY