A capillary electrophoresis detection system and detection method

Abstract

Provided are a capillary electrophoresis detection system and a detection method. The device comprises a laser set (101), a capillary set (102) and a detector set (103). Each laser in the laser set (101) sequentially irradiates each capillary in the capillary set (102), a detection sample in each capillary comprises target substances to be detected, the target substances to be detected comprise biological substances labelled with a fluorescent dye or biological substances bound with an antibody labelled with a fluorescent dye, each laser sequentially irradiates each capillary, fluorescent light in a plurality of wavelength ranges generated by the excitation of the fluorescent dye in each capillary is received by a detector provided for the laser, and then the types or contents of the substances contained in the detection sample in each capillary are sequentially detected. The sequential irradiation of a plurality of capillaries using a plurality of lasers can detect detection samples of different types or sources one time and can also detect a plurality of different target substances to be detected of the same type and source in the same detection sample one time, improving the detection speed and reducing the consumption of detection samples.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a capillary electrophoresis detection system and detection method. Background technique [0002] Capillary electrophoresis (capillary electrophoresis, CE) technology is a new type of liquid phase separation technology with capillary as the separation channel and high voltage direct current electric field as the driving force. Capillary electrophoresis generally uses a quartz capillary column. Under the action of a high-voltage electric field outside the capillary, the buffer on one side moves toward the negative electrode due to its positive charge. At the same time, in the buffer solution, the charged biomolecules move towards the electrode with the opposite polarity to the charged biomolecules at different speeds under the action of the high-voltage electric field outside the capillary, forming electrophoresis. [0003] In the prior art, the capillary electrophoresis techniq...

AI Technical Summary

Problems solved by technology

At present, the commonly used detection method is to use a laser to excite the fluorescence generated by the fluorescent dye on the detection sample in a capillary for detection. Only one target substance to be detected can be detected at a time, resulting in slow detection speed and high cost of detection samples.

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