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Method for quickly screening trace taxol active substances in plants

An active substance, paclitaxel technology, applied in the direction of organic chemistry, etc., can solve the problem that separation is difficult to achieve high-throughput screening of trace paclitaxel active substances

Active Publication Date: 2016-10-12
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods cannot separate in a short period of time Difficult to achieve high-throughput screening Trace paclitaxel active substances

Method used

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  • Method for quickly screening trace taxol active substances in plants
  • Method for quickly screening trace taxol active substances in plants
  • Method for quickly screening trace taxol active substances in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Weigh 0.25g of the yew needle leaf sample, extract it ultrasonically with methanol, and inject the extract into the injector directly from a micro-syringe after passing through a 0.45μm filter membrane, move the valve of the injector to complete the sample injection; disconnect the valve 1 And valve 2, adjust the syringe pump controller so that pump 1 pushes the first mobile phase at a flow rate of 2.5mL / h, and the sample enters the unactivated carbon fiber micro-column and the activated carbon fiber micro-column driven by the first mobile phase , the components of different polarities in the methanol extract of yew needles have different action mechanisms with the two micro-columns, and are selectively retained on different micro-columns; (3) after the first mobile phase is eluted, the obtained The eluent of the first component enters the tandem mass spectrometer online to complete the ionization process, and realizes the screening of strongly polar paclitaxel precursor...

Embodiment 2

[0083]Weigh 0.25g of Pleurotus ostreatus sample, extract it ultrasonically with methanol, and inject the extract into the sampler directly from a micro-syringe after passing through a 0.22μm filter membrane, move the valve of the sampler to complete the sample injection; disconnect valve 1 and valve 2. Adjust the syringe pump controller so that pump 1 pushes the first mobile phase at a flow rate of 1.5mL / h. Driven by the first mobile phase, the sample enters the unactivated carbon fiber micro-column and the activated carbon fiber micro-column. The components of different polarities in the mushroom methanol extract have different mechanisms of action from the two micro-columns, and are selectively retained on different micro-columns; (3) The first component eluate enters the tandem mass spectrometer online to complete the ionization process. (4) Close valve 1, disconnect valve 2 and adjust the syringe pump so that pump 2 pushes the second mobile phase to elute the activated carb...

Embodiment 3

[0085] Weigh 0.25g of morning glory sample, ultrasonically extract it with methanol, pass the extract through a 0.65μm filter membrane, inject it into the sampler directly from a micro syringe, move the valve of the sampler, and complete the sample injection; disconnect valve 1 and valve 2. Adjust the syringe pump controller so that pump 1 pushes the first mobile phase at a flow rate of 3.5mL / h. Driven by the first mobile phase, the sample enters the unactivated carbon fiber micro-column and the activated carbon fiber micro-column. The components of different polarities in the bovine methanol extract have different mechanisms of action from the two micro-columns, and are selectively retained on different micro-columns; Enter the tandem mass spectrometer to complete the ionization process and realize the screening of strong polar paclitaxel precursors; (4) close valve 1, disconnect valve 2 and adjust the syringe pump so that pump 2 pushes the second mobile phase to elute the act...

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Abstract

The invention provides a method for quickly screening trace taxol active substances in plants. The method comprises the steps that micro-columns filled with unactivated carbon fibers and activated carbon fibers with different surfaces from those of the unactivated carbon fibers are used for carrying out pre-separation on taxus mairei extract, different mobile phases are used for elution, and taxol active substances with different polarities are obtained; by means of an online ionization process, the taxol active substances represented by baccatin III, 10-deacetylation baccatin III, taxol, 7-xylosyl-10 deacetylation taxol, 7-xylosyl-taxol, cephalomannine and 7-epi-10-deacetylation taxol are screened, the whole screening process only needs 5 min, and the screening detection limit reaches the level of 25 ng / g; by means of the method, the material dosage for analysis and background interference are reduced, the detection sensitivity is improved, and the high universality of screening various natural samples with different ingredients is achieved.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for rapidly screening trace paclitaxel active substances in plants. Background technique [0002] Paclitaxel is a kind of diterpenoid compound with anti-cancer effect isolated from Taxus genus. The basic skeleton of paclitaxel can be converted into paclitaxel by effective methods, and more and more attention has been paid to it. [0003] At present, the specific process of the common method for the separation and detection of paclitaxel in plants is as follows: extract with ethanol, shake and layer with water and dichloromethane solvent, then evaporate the dichloromethane layer to dryness, add n-hexane and methanol for shaking, and concentrate the methanol layer Then enter the detector for detection; or after extraction, it is purified by a solid-phase column, eluted with a large amount of solvent, concentrated, and then enters a high-efficiency liqui...

Claims

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Application Information

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IPC IPC(8): C07D305/14
CPCC07D305/14
Inventor 李东浩马标邹依霖
Owner YANBIAN UNIV
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