Goose oaz1 polypeptide antigen and its polyclonal antibody preparation method and application

Abstract

The invention provides a goose OAZ1 (ornithine decarboxylase antizyme 1) polypeptide antigen as well as a preparation method and an application of a polyclonal antibody of the goose OAZ1. On the basis of a goose OAZ1 sequence, a +1 frame shift site of a goose OAZ1 gene is identified, and a segment of specific polypeptide sequence is screened and identified behind the +1 frame shift site. The method comprises steps as follows: 1, OAZ1 sequence analysis as well as screening and identification of a polypeptide antigen sequence; 2, N-terminal modification, synthesis and detection of the OAZ1 polypeptide antigen sequence; 3, antiserum preparation from OAZ1 polypeptide antigen immunized New Zealand rabbits and detection of titer of antiserum; 4, purification of the OAZ1 antibody. The OAZ1 polypeptide antibody prepared with the method can be specifically bound with OAZ1 full-length function protein in goose tissue, fills up the blank in the field of study on goose OAZ1 protein detection, and lays the foundation of research of goose OAZ1 functions and animal reproduction regulation functions by means of polyamine.

Description

technical field [0001] The invention belongs to the fields of biochemistry and molecular immunology, and relates to a preparation method and application of a goose OAZ1 polypeptide antigen and its polyclonal antibody. The prepared antibody has the advantages of simple and fast preparation method, low cost, high potency and specific binding Characterization of the OAZ1 protein in goose tissues. Background technique [0002] Polyamines (including putrescine, spermidine and spermine) are a class of low-molecular-weight aliphatic compounds with two or more amino groups, which can participate in the regulation of gene expression and translation, cell proliferation, cell signal transduction, cell membrane stabilization process. Low levels of polyamines can lead to inhibition of cell growth or even death, but abnormally high levels of polyamines can also lead to cancer. Therefore, maintaining the homeostasis of polyamines in cells is of great significance for normal cell growth. ...

AI Technical Summary

Problems solved by technology

However, there is currently a lack of commercialized poultry OAZ1 antibodies, and the preparation process of OAZ1 monoclonal antibodies is complicated, time-consuming, and expensive, so that the research on OAZ1 function only stays at the gene level, which seriously hinders the research on OAZ1 and polyamines regulating animal reproductive function.

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