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Antisense microrna-25 and its application

A microRNA-25, antisense technology, applied to antisense microRNA-25 and its application field

Active Publication Date: 2018-12-18
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lack of research on the mechanism of oxidative damage in RPE cells

Method used

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  • Antisense microrna-25 and its application
  • Antisense microrna-25 and its application
  • Antisense microrna-25 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of antisense microRNA-25, it is the antisense RNA of microRNA-25, and it is the antisense RNA of microRNA-25, and its base sequence is as shown in SEQ ID NO.1, and the sequence of microRNA-25 is as shown in SEQ ID NO .2 shown.

[0026] The antisense microRNA-25 can be obtained by artificial synthesis, which is a conventional technical means.

Embodiment 2

[0028] In this embodiment, cobalt chloride (CoCl 2 ) and Oxygen Glucose Deprivation (OGD) model (OxygenGlucoseDeprivation, OGD) two methods to simulate in vitro hypoxia / oxidative stress conditions, respectively, to study the effects of two forms of oxidative damage on the expression of microRNA-25 in primary RPE cells and human RPE cell lines.

[0029] Primary RPE cells were deprived of glucose and oxygen for 12, 16, 18, and 20 hours, and reoxygenated for 2 hours. The expression of miR-25 was detected by qRT-PCR. It was found that the expression of miR-25 increased with the prolongation of glucose and oxygen deprivation.

[0030] After the primary RPE cells were treated with different concentrations of cobalt chloride solution for 12 hours, the expression of miR-25 was detected by qRT-PCR, and it was found that the expression of miR-25 was up-regulated with the increase of the treatment concentration.

[0031] Primary RPE cells were treated with 200 μM cobalt chloride solution...

Embodiment 3

[0035] Taking mouse oxygen-induced retinopathy (OxygenInduced Retinopathy, OIR) as the model of hypoxia / oxidative stress in vivo, the expression of microRNA-25 in the neural retina and RPE / choroid complex was detected by qRT-PCR in the normoxia group and the hypoxia group The expression difference was further confirmed by in vivo experiments on the induction effect of hypoxia / oxidative stress on microRNA-25.

[0036] Construction method of OIR mouse model:

[0037] On the 7th day after birth, the mice in the model group were placed in the animal experiment cabin connected to the oxygen meter along with 2 nursing mothers, and oxygen (O 2 ), so that the oxygen concentration is stably controlled at 75% ± 2%. Daylighting. Regularly open the oxygen chamber every day to clean, change the dressing (keep it dry), change food and add water, and replace the nursing mothers in the normal environment with the mothers in the cabin. On the 12th day after the mice were born, the mice in t...

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Abstract

The invention relates to an antisense microRNA-25, a vector containing an antisense microRNA-25 base sequence, a composition containing the antisense microRNA-25 and an application of the antisense microRNA-25 composition as a drug for intervening expression of key oxidative stress related factors. Compared with the prior art, the antisense microRNA-25 and the corresponding vector or composition thereof provided by the invention can intervene the expression of the key oxidative stress related factors with multiple targets, and can be used in drugs for treating or preventing anti-oxidative damage, tumor resistance, heart failure, age-related macular degeneration, diabetic retinopathy and other oxidative stress related diseases.

Description

technical field [0001] The invention relates to an oxidative stress-related molecule, in particular to antisense microRNA-25 and its application. Background technique [0002] Age-related macular degeneration (AMD), which can cause irreversible damage to vision, is a complex multifactorial disease, and currently there is no effective treatment for the cause. Oxidative stress is one of the main pathogenesis of AMD, and retinal pigment epithelium (RPE) cells are the initial pathological cells. However, there is a lack of research on the mechanism of oxidative damage in RPE cells. Contents of the invention [0003] The purpose of the present invention is to provide antisense microRNA-25 and its application. [0004] When the present invention explores the role and mechanism of microRNA-25 in the oxidative damage of RPE cells, it is found that cobalt chloride (CoCl 2 ) and Oxygen Glucose Deprivation (OGD) model (Oxygen Glucose Deprivation, OGD) to simulate in vitro hypoxia / ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K31/7105A61K48/00A61P39/06A61P35/00A61P9/04A61P27/02A61P9/14A61P3/10
CPCA61K31/7105C12N15/1136
Inventor 徐国彤吕立夏张介平
Owner TONGJI UNIV
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