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A preparation method of microbial flocculant for purification of cultured seawater

A microbial flocculant and seawater purification technology, which is applied in the field of biotechnology and bioengineering, can solve the problem of poor removal of soluble organic matter, inorganic matter and total N, P, bacteria and pollutants cannot be effectively aggregated, and cannot be fully utilized. Response effect and other issues, to achieve good application value, low production cost, speed up the effect of flocculation and sedimentation

Active Publication Date: 2019-09-13
QINGDAO YAODONG GRP
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AI Technical Summary

Problems solved by technology

[0003] The current physical treatment technology, such as sedimentation and filtration, can remove suspended solids and some COD and BOD in aquaculture seawater, but the removal effect on soluble organic matter, inorganic matter and total N, P, etc. is not good
Chemical treatment methods, such as bleaching powder, malachite green and other water quality improvers, although they can also treat the seawater after cultivation, but these methods will cause secondary pollution to the water quality environment
The ozone method is a treatment method that can eliminate pollutants in water, improve water quality and be environmentally friendly, but the disadvantage of ozone oxidation technology is that the treatment cost is high, and the residual ozone will also produce certain toxicity to the breeding objects effect
However, due to the low pollutant load in aquaculture seawater and the small number of functional bacteria, bacteria and pollutants cannot be effectively aggregated, so a good reaction effect cannot be exerted.
[0004] Chinese Invention Patent "Denitrification Purification Method for Mariculture Wastewater" (Applicant: Dalian University of Fisheries, Application No.: 200810230123.9) and Chinese Invention Patent "Preparation Method of Seawater Microbial Flocculant" (Applicant: State Oceanic Administration, Application No.: 201010247147.2 ) discloses a biological treatment method for aquaculture seawater, but it has the disadvantages of single effect, difficulty in large-scale application, long production cycle, and large amount of treatment agent

Method used

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  • A preparation method of microbial flocculant for purification of cultured seawater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Microbial flocculant preparation and expanded cultivation of production strains

[0031] (1) Expanded cultivation of production strains

[0032] ① Medium

[0033] Klebsiella (Klebsilla sp.) LDX1-1 slant medium: sucrose 12g / L, NH 4 NO 3 1.5g / L, K 2 HPO 4 1.0g / L, MgSO 4 0.4g / L, NaCl 0.3g / L, FeSO 4 0.01g / L, agar powder 1.5%.

[0034] Xanthomonas campestris CICC10258 slant medium: peptone 5g / L, beef extract 3g / L, sodium chloride 5g / L, agar powder 1.5%.

[0035] Shake flask culture medium: (sucrose 60%+glucose 40%) 15g / L, mixed nitrogen source (NH 4 NO 3 55%+((NH 4 ) 2 SO 4 45%) 3g / L, K 2 HPO 4 1.2g / L, MgSO 4 0.6g / L, NaCl 0.3g / L, FeSO 4 0.01g / L.

[0036] ②Inoculation and cultivation

[0037] Slant strain culture: inoculate Klebsiella LDX1-1 (CGMCC No.11330) and Xanthomonas campestris CICC10258 into the slant medium respectively, and culture at 24-32°C for 24-48h until the slant strains grow vigorously ;

[0038] Expanded cultivation of s...

Embodiment 2

[0039] Example 2 Microbial flocculant fermentation

[0040] (1) Fermentation culture (high concentration magnesium salt fermentation)

[0041] Fermenter medium: sucrose 26g / L, cornstarch 15g / L, mixed nitrogen source (NH 4 NO 3 55%+((NH 4 ) 2 SO 4 45%) 2.7g / L, K 2 HPO 4 1.1g / L, MgSO 4 10g / L, NaCl 0.3, FeSO 4 0.015g / L, MnSO 4 0.03g / L.

[0042] Fermentation medium is inoculated with Klebsiella LDX1-1, Xanthomonas campestris CICC10258 mixed shake flask expansion culture liquid bacterial classification by volume ratio 2.2% inoculum (Klebsiella in the mixed shake flask expansion culture liquid bacterial classification Bacillus LDX1-1, Xanthomonas campestris CICC10258 expansion culture liquid strain volume ratio is 6:4). Fermentation adopts high-concentration magnesium salt fermentation, and the amount of magnesium sulfate required for fermentation is added to the cultured fermentation medium in the proportion of 20%, 35%, and 45% of the total amount when preparing th...

Embodiment 3

[0045] Embodiment 3: produce solid microbial flocculant

[0046] The liquid microbial flocculant of Example 1 was added 2.0 times the volume of 95% (V / V) ethanol under 70r / min full stirring to obtain a precipitate, which was centrifuged with a centrifuge for 8min, and the resulting solid was washed with 90% (V / V) V) Fully wash with ethanol, dry the alcohol-containing solids with a vacuum desiccator at a vacuum degree of -0.07Mpa and 42°C until the moisture is less than 5%, and pulverize them into 80 mesh powders with a pulverizer. The yield is 2.6%, and the flocculation rate is ≥93%.

[0047] The flocculant was prepared by single-bacteria fermentation using Klebsiella LDX1-1 (using the method in Example 2 of patent application 201510656846.5) for comparison, and the results are shown in Table 1.

[0048] Table 1 The present invention is compared with the product effect of Klebsiella LDX1-1 single bacterium fermentation

[0049]

[0050] As can be seen from Table 1: the fe...

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Abstract

The invention discloses a preparation method of a microbial flocculant for purifying aquaculture seawater. The preparation method is characterized by carrying out synergistic fermentation on a mixed strain of klebsiella LDX1-1 and xanthomonas campestris CICC10258 with fermentation of a high-magnesium fermentation culture medium by adopting an antagonism-free compatible shake flask, and carrying out gelation strength treatment by adopting a salt mixture after the fermentation is completed. The preparation method disclosed by the invention is fast in fermentation speed, the technology can be easily operated, the fermentation concentration is high, and the production cost is low; the produced flocculant is used for treating high-salinity high-buoyancy seawater, has the characteristics of stable structure, flocculating block compactness and fast settling speed, and has an obvious effect in purifying the aquaculture seawater.

Description

technical field [0001] The invention relates to a preparation method of a microbial flocculant for purification of cultured seawater, belonging to the technical fields of biotechnology and bioengineering. Background technique [0002] With the rapid development of China's mariculture industry, the discharge of wastewater from coastal hatcheries and farms is increasing day by day, resulting in the deterioration of water quality in coastal waters, or eutrophication and imbalance of the ecosystem, leading to breeding diseases and frequent red tides. The pollution of coastal waters seriously damages the marine ecological environment and affects the sustainable economic development of vast coastal areas. Mariculture wastewater includes particulate solid waste from feces and feed, dissolved metabolic waste, inorganic salts, antimicrobial agents and drug residues, etc. Mariculture wastewater is different from industrial wastewater and domestic sewage in that it has the characteris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P1/04C02F3/34C12R1/22C12R1/64C02F103/20C02F103/08
Inventor 栾兴社姜国辉
Owner QINGDAO YAODONG GRP
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