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Method for discriminating rhizoma dioscoreae nipponicae medical materials

A technology for yam and wild yam, which is applied in the field of identification of Dioscorea chuanensis and wild yam, can solve problems such as unsatisfactory, lack of identification standards and distinctions.

Inactive Publication Date: 2016-10-12
KOREA INST OF ORIENTAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this way, it is used without distinguishing related species because there is no standard for identifying them. Although Dioscorea chinensis and wild yam are different in appearance, they are distributed in a dried and cut state, so it is difficult to use the difference in appearance during distribution. to make a substantive distinction
[0005] Therefore, only by working out the identification method of Dioscorea chinensis and wild yam can the Chinese medicine be used correctly, but so far, no correct identification standard between them has been worked out
That is, as described above, a method for distinguishing Dioscorea lanceolata and wild yam as a method for distinguishing differences between related species of medicinal plants is urgently needed, but it has not been satisfactory so far.
[0006] As an example of prior art on Dioscorea chinensis and wild yam, Chinese Patent No. 101791366 discloses a quality inspection method for Dioscorea chinensis and medicinal materials, and Korean Patent No. 0854621 discloses a Dioscorea family Extracts of plants and compositions containing them for the prevention or treatment of peripheral nerve diseases, Korean Patent No. 0684434 disclose compositions containing wild yam extracts which have the function of inhibiting antioxidant, lipid Anti-aging, anti-inflammation and expectorant activities achieved by peroxidation of substances. Korean Patent No. 1332360 discloses a composition for preventing and treating fruit storage disease comprising Pangosaurus extract and microbial strains, but there is no report on the easy identification of Pangosia. The technology of the identification method of Long Yam and wild yam

Method used

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  • Method for discriminating rhizoma dioscoreae nipponicae medical materials

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1. Morphological analysis of Dioscorea nigra and wild yam

[0039] In this Example 1, the internal structure was analyzed morphologically using collected and circulating products of Dioscorea nigra and wild yam.

[0040] The completely dried medicinal materials were cut into a thickness of about 3 mm, and for the restoration and softening of the dried tissue, pretreatment was performed in boiling water at 100°C for 10 minutes. After that, in order to dehydrate the tissues, 10%, 20%, 35%, 50% and 75% of thiobarbituric acid were treated in a manner of 4 hours each in each step. Finally, 100% Thiobarbituric acid was treated twice for 4 hours each time. Under the temperature condition of 60°C, the dehydrated tissue was treated with 100% paraffin (paraffin, melting point 56-58°C) twice for 4 hours each time, and then embedding. The embedded tissue was cut into a thickness of 10-20 μm using a Leica flat push microtome (Leica SM 2010R), and the tissue section was adhered ...

Embodiment 2

[0048] Example 2. Extraction of Dioscorea nigra and Wild Yam

[0049] As the dragon yam and wild yam, collected products and circulating products (CM1 to CM5) collected from Wangsan, Sancheong-gun, Gyeongsangnam-do, were used to identify the collected products in the Korean Medicine Resource Group of the Korean Academy of Oriental Medicine , And make medicinal material specimens and compressed plant specimens for storage.

[0050] In addition, in order to use the control group for comparison, the Chinese and Korean circulation products of yam (yam) and the Chinese circulation products of Dioscorea nigra (Dioscorea nigra) were purchased and used.

[0051] For material analysis, the roots of Dioscorea nigra, wild yam, and yam (yam) were washed with running water, washed again with distilled water 3 times, and dried in a desiccator at 45°C for 1 week before cutting them (2.5~5.0cm) ). After cutting, dry again for 1 week, and apply the method of Jin et al. (Integrative Medicine Researc...

Embodiment 3

[0052] Example 3. High performance liquid chromatography analysis of Dioscorea nigra and wild yam

[0053] The extracts of Dioscorea nigra, wild yam, and yam (yam) obtained in Example 2 were used for high performance liquid chromatography analysis.

[0054] For high performance liquid chromatography reagents (distilled water, acetonitrile, etc.), HPLC grades are used. Use a high performance liquid chromatograph (waters Alliance 2695 system) equipped with a photodiode array detector (2996photodiode array detector) and a Phenomenex Luna C18column (250mm×4.6mm; particle size 5μm) , Analysis at 254nm.

[0055] Use 0.1% (v / v) trifluoroacetic acid aqueous solution (A) and acetonitrile (B) as mobile phases at a ratio of 90% (A): 10% (B) for 10 minutes, from 10 minutes to 60 minutes was performed by changing the gradient at a ratio of 40% (A): 60% (B), and the temperature was maintained at 40° C., the flow rate was 1.0 ml / min, and the sample injection volume was 20 μl for analysis.

[0056]...

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Abstract

The present invention relates to a method for identifying Dioscorea chinensis and wild yam. diosgenin content; and (b) discriminating wild yam and Dioscorea punctatus by said stained tissue, wherein the periderm is dyed red, and only the cork layer is composed of wild yam; the periderm is dyed blue color, and composed of cork layer, cork cambium and cork cortex is Dioscorea chinensis, or the content (mg) of diosgenin measured based on the dry weight (g) of the sample is used to identify the diosgenin. Dragon yam or wild yam.

Description

Technical field [0001] The present invention relates to a method for identifying Dioscorea nigra and wild yam circulating in the name of Dioscorea nigra and, more specifically, to a method for identifying Dioscorea macrophylla and wild yam by morphological and physical identification of circulating products of Dioscorea nigra. Background technique [0002] Dioscorea nipponica Makino (Dioscorea nipponica Makino) is a perennial vine that grows in mountainous areas. It is a medicinal plant with horizontal stems and sparse branches. The rhizome is characterized by a cylindrical shape with many roots. The leaves diverge and are wide oval or heart-shaped, with a length of 7-15 cm and a width of 4-12 cm. The tip of the leaf is long and the bottom is heart-shaped. Usually, the leaves split into the palm shape, and some do not split, but it is very rare. The petiole is long and there are no bulbous buds on the leaf axils. In July-August, it blooms as a dioecious plant. The male inflor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 姜泳泯金东善崔高也梁善圭
Owner KOREA INST OF ORIENTAL MEDICINE
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