New Carbon Fiber Electrodes for Patch Clamp Electrochemical Technique Systems

Abstract

The invention belongs to the technical field of patch clamps, and specifically relates to a novel carbon fiber electrode, which is suitable for the patch clamp electrochemical technology and is used to collect electrochemical signals of a clamped patch in a glass micro tube. Silicone rubber is taken as the insulation material. A high signal to noise ratio is maintained, at the same time, the conventional high temperature heating method and dependence on equipment and operation are avoided, and the preparation method is simple and easy to perform. The sleeve tube is a polypropylene tube with a small external diameter (about 0.2 millimeter), the whole polypropylene tube is small, and thus the polypropylene tube can move easily in a glass micro tube. Due to the design of tail end, the conduction and connection mode of electrical signals becomes simpler, the carbon fiber electrode can be replaced through a plugging mode, and the operation is easy.

Description

technical field [0001] The invention belongs to the field of patch clamp technology. Specifically, it relates to a new type of carbon fiber electrode, which is suitable for the patch clamp electrochemical technology, and collects electrochemical signals from the clamped membrane in the glass microtube. Background technique [0002] Patch clamp technique and electrochemical technique are commonly used methods to study the mechanism of synaptic activity. In order to more accurately answer many questions in the process of single vesicle fusion and recycling, in 1997, the Lindau research group of the Max Planck Institute in Germany combined the cell-attached membrane capacitance recording method in the patch clamp technique with the electrochemical recording method , created the patch amperometry technique. In this technique, carbon fiber electrodes are inserted into the glass microtube and close to the clamped membrane, and the release of neurotransmitters can be recorded ele...

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Problems solved by technology

[0003] Carbon fiber electrodes are the core components of this technology for recording neurotransmitter release. Due to their use environment is different from conventional electrochemical recording systems, some areas for improvement have also been highlighted in recent use: the carbon fiber electrodes used in this technology The preparation adopts the polyethylene pipe (built-in carbon fiber) melting method, which requires the purchase of special high-temperature heating equipment and is relatively dependent on the opponent's method; as one of the raw materials, the size of the polyethylene pipe is relatively large (outer diameter 0.61 mm), and it needs to be combined with another A silver wire used as a ground wire is inserted side by side into a glass microtube with an inner diameter of 1.16 mm, so it is easy to be blocked when the carbon fiber electrode moves back and forth in the glass microtube; use potassium chloride solution as a conductive medium to connect the carbon fiber and the silver wire for recording, and conduct electricity Compared with metals, the property is poor, and it is prone to liquid leakage and short circuit, and it is easy to precipitate crystals during storage, which will affect reuse

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