Detoxification tissue culture and rapid propagation method of lily
A technology for tissue culture and rapid propagation of lilies, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as provenance degradation, disease, and lack of lily bulbs, and achieve increased adventitious bud differentiation and shortened growth Period, the effect of making up for the lack of breeding
Inactive Publication Date: 2016-10-26
张万萍 +1
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[0003] The object of the present invention is to provide a lily virus-free tissue culture and rapid propagation method, aiming to solve the problems of lily bulb shortage, provenance degradation and serious disease
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[0012] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0013] The lily detoxification tissue culture rapid propagation method provided by the present invention adopts explant sterilization: soak in 75% ethanol for 30 seconds, then disinfect with 10% sodium hypochlorite for 20 minutes; detoxification by heat treatment: alternate heat treatment at 26°C and 45°C for 1 month , peel off 0.2-0.8mm shoot tips; domesticated transplanting substrate: perlite: vermiculite: peat (1:1:1) and disinfect it twice with boiling water.
[0014] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.
[0015] like figure...
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The invention discloses a detoxification tissue culture and rapid propagation method of lily. The method is characterized in that an explant comprises an inner scale and a stem bud; stem apex detoxification includes performing alternating thermal treatment of 26 DEG C and 45 DEG C for 1 month and stripping 0.2-0.8mm of the stem apex. The method has the advantages that the growth period of the lily is shortened, and industrialized seedling production of the lily is achieved; high-quality detoxified seedlings are obtained through the stem apex detoxification technology, the problems of lily bulb insufficiency, provenance degeneration and serious diseases are solved effectively, lily propagation coefficient and quality are increased, economic benefits are increased, the industrialized and standardized development of the lily is promoted, the popularization of good varieties is accelerated, breeding defects are overcome, and distant hybridization new varieties can be obtained; the inner scale and the stem bud cannot be polluted easily, a good sterilizing effect is achieved, high survival rate and adventitious bud differentiation number are achieved, and the method can be the optimal choice for explant tissue culture and rapid propagation of Lilium lancifolium.
Description
technical field [0001] The invention belongs to the technical field of lily reproduction, and in particular relates to a method for rapid propagation of lily by tissue culture and virus-free. Background technique [0002] Edible lilies have been propagated asexually by vegetative bodies such as bulbs, scales, and bulbils for a long time. There are problems such as low reproduction coefficient, high degree of virus infection, and frequent bulb degradation, which seriously affects the quality and output of lily, and thus also affects the lily industry. development of. Contents of the invention [0003] The object of the present invention is to provide a lily virus-free tissue culture and rapid propagation method, aiming to solve the problems of lily bulb shortage, provenance degradation and serious disease. [0004] The present invention is achieved in this way, a lily detoxification tissue culture rapid propagation method, the explants of the lily detoxification tissue cul...
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IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 张万萍牛力立杨雪莲裴芸
Owner 张万萍