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Method for simultaneously detecting oxytetracycline, tetracycline and kanamycin based on ABEI modified flower-shaped nanogold

A kanamycin and nano-gold technology, applied in the fields of nanomaterials and analytical chemistry, can solve the problems of increasing markers or labeling signals, increasing the difficulty of detection methods, restricting target types, etc., to improve stability and accuracy, and to facilitate Chemical modification, the effect of avoiding errors

Active Publication Date: 2016-10-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, synthesizing multicolor-labeled nanomaterials is not a simple task, which greatly increases the difficulty of detection methods.
In addition, it is difficult to increase the number of markers or labeling signals during the synthesis of multicolor-labeled nanomaterials, which largely restricts the types of targets in multi-component detection.

Method used

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  • Method for simultaneously detecting oxytetracycline, tetracycline and kanamycin based on ABEI modified flower-shaped nanogold
  • Method for simultaneously detecting oxytetracycline, tetracycline and kanamycin based on ABEI modified flower-shaped nanogold
  • Method for simultaneously detecting oxytetracycline, tetracycline and kanamycin based on ABEI modified flower-shaped nanogold

Examples

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Embodiment 1

[0023] Example 1: Detection of oxytetracycline, tetracycline and kanamycin in actual milk samples. Test sample pretreatment: first, add different concentrations of oxytetracycline, tetracycline, and kanamycin to milk samples, and dilute 40 times with ELISA treatment solution. Three different brands of milk were purchased from a local supermarket, and the content of ochratoxin A was determined by using the method of the present invention and the enzyme-linked immunosorbent method, and the results are shown in Table 1. The detection results of the two methods were consistent with no significant difference.

[0024]Table 1: The actual sample detection of milk, the method of the present invention is compared with the ELISA method

[0025]

[0026] Note: ND is not detected

Embodiment 2

[0027] Example 2: Detection of Oxytetracycline, Tetracycline and Kanamycin in Milk Actual Samples and the Experimental Sample Pretreatment of Standard Addition Recovery Rate is the same as in Example 1.

[0028] With the 3 groups of oxytetracycline that embodiment 1 obtains, tetracycline and kanamycin concentration data are background values, add three kinds of antibiotic standard substances of different concentrations wherein respectively, utilize the method of the present invention to detect again the antibiotic added wherein content to obtain the detection value. Recovery %=(detection value-background value) / addition amount X100%. From the data in Table 2, it can be seen that the recovery rate is 96.04% to 102.66%, indicating that the present invention is stable, sensitive and accurate, and is applicable to the detection of oxytetracycline, tetracycline and kanamycin in actual milk samples.

[0029] Table 2: Detection of oxytetracycline, tetracycline and kanamycin in actua...

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Abstract

Provided is a method for simultaneously detecting oxytetracycline, tetracycline and kanamycin based on ABEI modified flower-shaped nanogold. The method is characterized by comprising the steps that the chemiluminescence time can be effectively prolonged based on a N-(4-ammonia butyl)-N-ABEI-H2O2-p-iodophenol (PIP) steady-state chemiluminescence system constructed by a microwell plate; a sulfhydrylation complementary chain is connected with ABEI modified flower-shaped nanogold to be used for a signal probe; aptamers fixed on the microwell plate are used as molecular recognition elements, and the aptamers of three antibiotics are fixed on three regions of the microwell plate respectively to provide spatial resolution for simultaneous detection of targets. The constructed aptamer sensor can achieve simultaneous detection on oxytetracycline, tetracycline and kanamycin, and the chemiluminescence intensity of the sensor is in negative logarithm correlation with the quantity of the targets. In addition, the sensor is used for practical sample detection, and the good property of the sensor is proved.

Description

technical field [0001] A method for the simultaneous detection of three antibiotics, oxytetracycline, tetracycline and kanamycin, based on N-(4-aminobutyl)-N-ethylisoluminol (ABEI)-modified flower-shaped gold nanoparticles and microwell plates The method relates to the technical fields of nanomaterials and analytical chemistry, and is used for detecting oxytetracycline, tetracycline and kanamycin in food. Background technique [0002] Oxytetracycline and tetracycline belong to tetracycline antibiotics, and kanamycin belongs to aminoglycoside antibiotics. They all belong to spectrum antibiotics and are widely used in animal husbandry. However, the abuse of these antibiotics will not only cause damage to animals themselves, but also have adverse effects on humans, because antibiotics will be enriched during the food chain. These side effects include damage to hearing, damage to kidneys, cause allergic reactions, and induce drug resistance in humans and animals. In addition, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 王周平郝丽玲顾华杰段诺吴世嘉马小媛夏雨
Owner JIANGNAN UNIV
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