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Kit for measuring lipoprotein phospholipase A2 and preparation method of kit

A technology for phospholipase and lipoprotein, which is applied in the field of a kit for determining lipoprotein phospholipase A2 and the field of preparation thereof, can solve the problems of low measurement accuracy, complicated operation and the like, and achieves high sensitivity, no radioactive pollution, and improved measurement accuracy. Effect

Inactive Publication Date: 2016-10-26
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a test kit for measuring lipoprotein phospholipase A2 and its preparation method in order to overcome the defects of complex operation and low measurement accuracy in the prior art

Method used

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  • Kit for measuring lipoprotein phospholipase A2 and preparation method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0066]Reagent R1:

[0067] MES buffer 100mmol / L

[0068] Potassium chloride 300 mmol / L

[0069] Polyethylene glycol-2000 10 g / L

[0070] EDTA·2Na 25 mmol / L

[0071] Sodium sorbate 0.6 g / L

[0072] The solvent is purified water

[0073] Reagent R2:

[0074] MES buffer 100 mmol / L

[0075] Bovine Serum Albumin 11 g / L

[0076] Sodium azide 0.7 g / L

[0077] Latex coated anti-lipoprotein phospholipase A2 antibody 5 g / L

[0078] Its solvent is purified water.

Embodiment 2

[0080] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0081] Reagent R1:

[0082] Tris buffer 20 mmol / L

[0083] Potassium sulfate 200 mmol / L

[0084] Polyvinylpyrrolidone 25 g / L

[0085] Diethyltriaminepentaacetic acid 45 mmol / L

[0086] Sodium sorbate 0.2 g / L

[0087] The solvent is purified water

[0088] Reagent R2:

[0089] Tris buffer 150 mmol / L

[0090] Bovine serum albumin 18 g / L

[0091] Sodium sorbate 0. 9 g / L

[0092] Latex coated anti-lipoprotein phospholipase A2 antibody 1 g / L

[0093] Its solvent is purified water.

Embodiment 3

[0095] Kit preparation and method of use

[0096] 1. Preparation of latex-coated anti-lipoprotein phospholipase A2 antibody: first dilute polystyrene microspheres with a particle size of 120 nm with 50 mmol / L MES buffer to obtain a mass concentration of polystyrene microspheres of 5% solution, and then add 1.0 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride in every milliliter of solution, react for 1 hour under the condition of 26 ℃, then Use a centrifuge to centrifuge at a speed of 25000 rpm / min for 30 minutes, remove the supernatant, dilute the precipitate with 50 mmol / L MES buffer, then use an ultrasonic disperser for ultrasonic dispersion, and then use a centrifuge to disperse at 25000 rpm Centrifuge for 30 minutes at a speed of 1 / min, remove the supernatant, and then dilute the precipitate with 50 mmol / L MES buffer until the mass concentration of polystyrene microspheres is 4%, use an ultrasonic disperser to disperse, and then Add anti-lipoprotein pho...

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Abstract

The invention discloses a kit for measuring lipoprotein phospholipase A2 and a preparation method of the kit. The kit comprises two liquid components including a reagent R1 and a reagent R2 which are mutually independent, the reagent R1 comprises buffer liquid, inorganic salt ion, accelerator, chelating agent and preservative, and the reagent R2 comprises buffer liquid, stabilizer, preservative and latex coated anti-lipoprotein phospholipase A2 antibody. The preparation method includes: preparing the reagents according to component content; mixing a to-be-measured sample with the reagents R1 and R2 for sufficient reaction; using a full-automatic biochemical analyzer to measure light absorbance difference value after reaction; calculating concentration of lipoprotein phospholipase A2 in the sample according to light absorbance change value. The kit has the advantages of high measuring accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring lipoprotein phospholipase A2 and a preparation method thereof. Background technique [0002] Lipoprotein phospholipase A2 (Lp-PLA2) is one of the subtypes in the phospholipase superfamily, also known as platelet-activating factor acetylhydrolase, which is secreted by macrophages, T cells and mast cells in the intima of blood vessels , the expression of Lp-PLA2 is up-regulated in atherosclerotic plaques, and it is strongly expressed in the macrophages of the fibrous cap of vulnerable plaques. Pro-inflammatory substances such as lysolecithin and oxidized free fatty acids, which in turn produce multiple atherogenic effects, including endothelial cell death and endothelial dysfunction, stimulate the production of adhesion factors and cytokines, which can be induced by chemotactic Inflammatory cells further create a self-reinforcing cycle, produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573G01N2333/916
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD