Preparation method of microelement chelated peptide and litopenaeus vannamei breeding feed

A technology of trace elements and chelated peptides, applied in animal feed, animal feed, additional food elements, etc., can solve the problem of immune tolerance in farmed animals, and achieve the effect of improving the growth and reproduction of organisms and enhancing disease resistance and immunity.

Inactive Publication Date: 2016-11-09
ZHEJIANG OCEAN UNIV
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Problems solved by technology

However, the use of immune additives will cause immune tolerance in farmed animals

Method used

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  • Preparation method of microelement chelated peptide and litopenaeus vannamei breeding feed
  • Preparation method of microelement chelated peptide and litopenaeus vannamei breeding feed
  • Preparation method of microelement chelated peptide and litopenaeus vannamei breeding feed

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Embodiment Construction

[0031] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0032] 1. Materials and methods

[0033] 1.1 Test material

[0034] Miscellaneous fish surimi. Ginger protease (enzyme activity 30000IU / g)

[0035] 1.2 Preparation of trace element chelating peptide

[0036] Take about 20g of miscellaneous fish surimi, dissolve it in 200mL deionized water, adjust the mixed slurry value to 7.0±0.1, keep it at 40°C for 20min; add ginger protease at a ratio of 30000U / g, and enzymolyze it in a water bath at 42°C for 6h; the enzymolysis process Adjust the enzymolysis solution with 1mol / L HCl / NaOH solution every 1h to control the pH at about 7.8.

[0037] After the enzymatic hydrolysis, heat in a water bath at 95°C for 10 minutes to i...

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Abstract

The invention discloses a preparation method of a microelement chelated peptide and litopenaeus vannamei breeding feed. The preparation method of the microelement chelated peptide comprises the following steps: 1) minced fish is made into pulp with the pH being 7.0 plus or minus 0.1 through enzymolysis and then left to stand, ginger protease is added, the mixture is subjected to a water bath at the temperature of 42 DEG C and stirred to be subjected to enzymolysis for 6 h, and the pH is controlled to be 7.8 plus or minus 0.1 in the enzymolysis process; 2) after hydrolysis and enzymolysis are finished, the mixture is heated for 10 min in a water bath at the temperature of 95 DEG C, the ginger protease is passivated, obtained hydrolysate is subjected to centrifugal separation, and an acquired middle clear liquid is a fish protein hydrolysate peptide solution; 3) microelements are added to adjust the pH value of the fish protein hydrolysate peptide solution to be 7.0, a 0.5 mol/L FeCl2 solution, a 0.5 mol/L ZnCl2 solution, a 0.5 mol/L CaCl2 solution and Vc are added, the mixture is oscillated in the water bath at the temperature of 35 DEG C for 25 min and subjected to centrifugal separation, and a supernatant is obtained; a nanofiltration membrane separation device is adopted, and the parts with the molecular weight being 1,000-5,000 Da are intercepted; the microelement chelated peptide is obtained after freeze drying.

Description

technical field [0001] The invention relates to a preparation method of a chelating peptide rich in trace elements and a culture feed for Litopenaeus vannamei using the chelating peptide. Background technique [0002] Litopenaeus vannamei is one of the main cultured prawns in the world, and it is also the main species of prawns cultured in China. In the process of intensive farming, the outbreak of infectious diseases is the main factor restricting the benefits of shrimp farming. Since the use of drugs to prevent diseases may have risks such as drug residues and bacterial drug resistance, it is an important development direction and research hotspot in nutritional feed science to improve the body's immune function and thus improve the body's disease resistance through nutritional means. Regarding the research on nutritional immunity of prawns, the existing reports mainly focus on the effects of immune additives such as vitamins, polysaccharides, and Chinese herbal medicines...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K20/189A23K20/147A23K10/22A23K10/37A23K20/158A23K10/30A23K20/174A23K20/20
CPCY02A40/818Y02P60/87
Inventor 张翰卿林慧敏邓尚贵
Owner ZHEJIANG OCEAN UNIV
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