Traditional Chinese medicine oral liquid for preventing and treating defensive vital energy syndrome of chicken
A traditional Chinese medicine oral liquid and qi separation technology, which is applied in the field of medicines for preventing and treating chicken Wei Qi syndrome, can solve problems such as slow onset of action, trachea and kidney damage in chickens, etc., and achieve fast onset, high recovery rate and high cure rate. Effect
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Embodiment approach 1
[0043] Embodiment 1 Baihu Dingchuan Oral Liquid Optimal Prescription Screening Experiment
[0044] 1 Experimental materials and methods
[0045] 1.1 Experimental materials
[0046] SPF chicks: 11 days old, provided by Shandong Haotai Experimental Animal Breeding Center.
[0047] SPF chicken isolator: produced in Changshu, Jiangsu.
[0048] IBV-M41 strain: Provided by Shandong Key Laboratory of Poultry Disease Diagnosis and Immunization, EID 50 1.0×10 -6.5 / 0.1mL.
[0049] Experimental drug: self-made 5 traditional Chinese medicine prescriptions, which are candidate prescriptions of Baihudingchuan Oral Liquid, and made a water decoction equivalent to 1g of crude drug per 1ml.
[0050] Recipe 1: 80g honeysuckle, 180g Radix Radix, 160g Scutellaria, 240g Gypsum, 100g Anemarrhena, 140g Campanulaceae, 50g fried almonds, 50g Fritillaria.
[0051] Prescription 2: 130g honeysuckle, 200g Radix Radix, 200g Scutellaria baicalensis, 240g raw gypsum, 180g Anemarrhena, 50g Fritillaria....
Embodiment approach 2
[0069] Embodiment 2 Baihudingchuan Oral Liquid Dose Screening Test
[0070] 1 test material
[0071] 1.1 Experimental drug: Baihudingchuan oral liquid, crude drug content 1g / ml, trial-manufactured by Shandong Haotai Technology Pharmaceutical Co., Ltd., batch number 20141201.
[0072] 1.2 Experimental animals: 17-day-old SPF chicks, provided by Shandong Haotai Experimental Animal Breeding Center.
[0073] 1.3 Virus strain: IBV-M41, purchased from the China Veterinary Drug Control Institute, preserved by the Key Laboratory of Poultry Disease Diagnosis and Immunization in Shandong Province, with an EID50 of 1×10 -6.5 / 0.1mL.
[0074] 1.4SPF Chicken Breeding Isolator: Suzhou Fengshi Experimental Animal Equipment Co., Ltd.
Embodiment approach 3
[0112] Embodiment 3 Baihudingchuan Oral Liquid Target Animal Safety Test
[0113] 1 test material
[0114] 1.1 Experimental drug: Baihu Dingchuan Oral Liquid, trial-produced by Shandong Haotai Science and Technology Pharmaceutical Co., Ltd., batch number 20141201.
[0115] 1.2 Test animals: 18-day-old SPF chicks, provided by Shandong Haotai Experimental Animal Breeding Center.
[0116] 1.3 SYSMEXKX-21: Automatic blood analyzer Sysmex Medical Electronics (Shanghai) Co., Ltd. product.
[0117] 1.4 Olympus AU400: automatic biochemical analyzer product of Olympus Corporation of Japan.
[0118] 2 test method
[0119] 2.1 Dose design: The target animal safety test should be based on the recommended clinical drug dose and set up no less than three dose groups, generally 1-fold, 3-fold, and 5-fold dose groups. According to the results of the dose screening test, we adopted 1ml per kg body weight as the recommended clinical dosage, so this test designed three dosage groups of 1ml p...
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