Biological method for removing urushiol sensitization of Rhus verniciflua Stokes
An allergy and biological technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as allergic reactions, development disadvantages of the raw lacquer industry, etc., achieve simple process operation, solve allergy problems, Easy to implement effect
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Embodiment 1
[0028] The first step, slant cultivation
[0029] Rapeseed yellow single cell Xanthomonas campestris Inoculate on a solid slant medium, the solid medium is: 5.0g peptone, 3.0g beef extract, 5.0g NaCl, 15.0g agar, 1.0L distilled water, pH7.0; Cultivate for 5 days to obtain slant strains;
[0030] The second step is to prepare the seed culture medium
[0031] Take the slant bacteria in the first step, under aseptic conditions, take 5 rings of bacteria, and transfer them to the liquid medium, the liquid medium is: 5.0g of peptone, 3.0g of beef extract, 5.0g of NaCl, Distilled water 1.0L, pH7.0; at 30-35°C, 180rpm shaker culture for 5-7 days to obtain yellow single cells of wild rape Xanthomonas campestris seed culture fluid;
[0032] The third step, biotransformation
[0033] Use the seed culture solution in the second step, add 0.4-1% urushiol at a volume ratio of 4-6%, inoculate the substrate medium, and culture on a shaker at 180rpm at 30-35°C for 1-2 days, ferment After...
Embodiment 2
[0038] The first step, slant cultivation
[0039] Streptomyces griseus var. rust Streptomyces griseus var. ferrugineus Inoculate on a solid slant medium, the slant medium is: 1.0L of potato extract, 20.0g of glucose, 15.0g of agar, natural pH; under the condition of 28-30°C, static culture for 5 days to obtain slant strains;
[0040] The second step is to prepare the seed culture medium
[0041] Take the slant bacteria in the first step, under aseptic conditions, take 5 rings of bacteria, and transfer them to the liquid medium, the liquid medium is 1.0L of potato extract, 20.0g of glucose, and the pH is natural; at 30~ Under the condition of 35°C, cultivate it on a shaker at 180rpm for 5-7 days to obtain the rust-colored variant of Streptomyces griseus Streptomyces griseus var. ferrugineus seed culture fluid;
[0042] The third step, biotransformation
[0043] Use the seed culture solution in the second step, add 0.4-1% urushiol at a volume ratio of 4-6%, inoculate the s...
Embodiment 3
[0045] The first step, slant cultivation
[0046] silvery mildew Cunninghamella elegans The strains were inoculated on a solid slant medium, the slant medium was: 1.0L of potato extract, 20.0g of glucose, 15.0g of agar, natural pH; at 28-30°C, static culture for 5 days to obtain slant strains;
[0047] The second step is to prepare the seed culture medium
[0048] Take the slant bacteria in the first step, under aseptic conditions, take 5 rings of bacteria, and transfer them to the liquid medium, the liquid medium is: 1.0L of potato extract, 20.0g of glucose, natural pH; at 30 Cultivate in a shaker at 180rpm at ~35°C for 5-7 days to produce Yazhi Xiaogram silvery mildew Cunninghamella elegans seed culture fluid;
[0049] The third step, biotransformation
[0050] Use the seed culture solution in the second step, add 0.4-1% urushiol at a volume ratio of 4-6%, inoculate the substrate medium, and culture on a shaker at 180rpm at 30-35°C for 1-2 days, ferment After the orig...
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