Method for increasing protein expression quantity by prolonging survival time of host cells of bombyx mori baculovirus expression system

Abstract

The invention belongs to the field of biotechnological pharmaceutics and relates to a method for increasing the protein expression quantity by prolonging survival time of host cells of a bombyx mori baculovirus expression system The bombyx mori baculovirus expression system with bombyx mori cells as hosts is a cracking expression system, the survival time of the host cells infected with viruses is very short, and the protein expression quantity is severely influenced. The host cells before virus infection are treated with an antioxidant, namely, grape seed proanthocyanidin, so that the survival time of the host cells after virus infection is prolonged, the expression level of protein is improved, the expression quantity is increased, meanwhile, protein obtained through expression can be modified and processed more sufficiently, accordingly, the protein has better biological activity, and the expression level of exogenous protein of larvae and silkworm chrysalis of bombyx mori treated with the method can be increased. A novel technical method is provided for increase of the protein expression quantity of the bombyx mori baculovirus expression system, and popularization and application of the method can bring great research and economic value in the protein expression industry.

Description

Technical field [0001] The invention belongs to the field of biotechnology pharmaceuticals, and specifically relates to a method for prolonging the survival period of host cells of the Bombyx mori baculovirus expression system and increasing protein expression. Background technique [0002] Currently, in the field of biotechnology, the baculovirus expression system has become a mainstream technology for foreign protein expression. In particular, the silkworm baculovirus vector expression system has many advantages in the production of recombinant proteins. [0003] In the Chinese Pharmacopoeia, the silkworm is a medicinal and edible insect. Using the silkworm as an expression vector and using baculovirus to express foreign proteins (including antimicrobial peptides) has unique advantages: ① The silkworm is larger in size, moderate in size, and easy to operate. Control, short growth period, strong protein synthesis ability, the main component of silk is protein; the amount of ...

AI Technical Summary

Problems solved by technology

[0005] The silkworm baculovirus expression system has defects in expressing foreign proteins because the expression system is a lytic virus expression system, which can easily lead to lysis and death of infected insect cells to terminate protein production

After baculovirus infects insect cells, the survival period of insect cells is only 5-6 days. After the cells are infected with the virus, they lose cell viability and stop the expression of foreign proteins, thus limiting the production of recombinant proteins.

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