Method for obtaining high-resolution two-dimensional J decomposition spectrum

A high-resolution, hydrogen spectrum technology, applied in the field of nuclear magnetic resonance spectroscopy detection, can solve the problems of signal excitation, inhomogeneous magnetic field applicability, etc., and achieve the effect of simple operation

Inactive Publication Date: 2016-11-09
XIAMEN UNIV
View PDF7 Cites 27 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is based on the multi-quantum coherence between heteronuclear molecules to obtain high-resolution NMR two-dimensional J-decomposition spectrum in any inhomogeneous magnetic field environment (including large inhomogeneous m

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for obtaining high-resolution two-dimensional J decomposition spectrum
  • Method for obtaining high-resolution two-dimensional J decomposition spectrum
  • Method for obtaining high-resolution two-dimensional J decomposition spectrum

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0043] see Figure 1~3 . The instrument used in this embodiment is a Varian NMR System 500MHz NMR spectrometer equipped with an effective detection length of 1.6cm, including an HCN triple resonance 5mm probe with an XYZ three-dimensional gradient field coil. The sample used in the experiment is a mixed solution of corn oil and deuterated acetone. The molar ratio is 1:10. According to the operation process described above, firstly, a one-dimensional hydrogen spectrum is obtained by sampling with a conventional one-dimensional pulse sequence, such as figure 2 shown. In the absence of any shimming operation before signal sampling, due to the influence of the magnetic field conditions of the instrument itself and the inhomogeneity of the intrinsic magnetic susceptibility of corn oil, a large non-uniform magnetic field with a half-height width of 620 Hz is generated, and the spectral resolution is too low at this time However, useful spectral information cannot be obtained, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for obtaining a high-resolution two-dimensional J decomposition spectrum, and relates to a nuclear magnetic resonance spectroscopy detection method. The method comprises: loading a sample to be detected into a standard nuclear magnetic test tube, and conveying into the detection chamber of a nuclear magnetic resonance spectrometer; sampling the one-dimensional hydrogen spectrum of the sample to be detected, and obtaining the signal peak distribution area and the spectral line width so as to provide the reference for the spectral width parameter setting, wherein the line width value reflects the magnetic field uniformity condition; measuring the pulse width of a radio frequency pulse required by excitation of the sample to be detected; introducing a compiled pulse sequence on the nuclear magnetic resonance spectrometer, opening the heteronuclear intermolecule multiple quantum coherent signal excitation module, the indirect dimensional evolving module, the spin echo fixed delay module and the J modulating fast sampling module of the pulse sequence, and setting the experiment parameters of various modules; skipping the artificial shimming operation process, and directly executing data sampling; and after completing the data sampling, calling data post-processing codes to carry out related data post-processing so as to obtain the high-resolution two-dimensional J decomposition spectrum being not affected by the inhomogeneous magnetic field.

Description

technical field [0001] The invention relates to a nuclear magnetic resonance (NMR, Nuclear Magnetic Resonance) spectroscopy detection method, in particular to a method for obtaining a high-resolution NMR two-dimensional J-decomposition spectrum in an arbitrary inhomogeneous magnetic field environment based on multi-quantum coherence between heteronuclear molecules. Background technique [0002] NMR spectroscopy can provide information such as chemical shifts and J-coupling constants for material composition analysis and molecular structure analysis, and is widely used in biochemistry, materials science, and life science research. Among them, the one-dimensional NMR spectroscopy method has important applications in chemical analysis and biological metabolism detection due to its fast signal sampling and intuitive spectral information. However, the sample system with complex components will inevitably bring a large number of NMR signals, which show phenomena such as signal cro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N24/08
CPCG01N24/08
Inventor 黄玉清陈忠蔡淑惠陈帅
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap