Method for obtaining high-resolution two-dimensional J decomposition spectrum

Abstract

The invention provides a method for obtaining a high-resolution two-dimensional J decomposition spectrum, and relates to a nuclear magnetic resonance spectroscopy detection method. The method comprises: loading a sample to be detected into a standard nuclear magnetic test tube, and conveying into the detection chamber of a nuclear magnetic resonance spectrometer; sampling the one-dimensional hydrogen spectrum of the sample to be detected, and obtaining the signal peak distribution area and the spectral line width so as to provide the reference for the spectral width parameter setting, wherein the line width value reflects the magnetic field uniformity condition; measuring the pulse width of a radio frequency pulse required by excitation of the sample to be detected; introducing a compiled pulse sequence on the nuclear magnetic resonance spectrometer, opening the heteronuclear intermolecule multiple quantum coherent signal excitation module, the indirect dimensional evolving module, the spin echo fixed delay module and the J modulating fast sampling module of the pulse sequence, and setting the experiment parameters of various modules; skipping the artificial shimming operation process, and directly executing data sampling; and after completing the data sampling, calling data post-processing codes to carry out related data post-processing so as to obtain the high-resolution two-dimensional J decomposition spectrum being not affected by the inhomogeneous magnetic field.

Description

technical field [0001] The invention relates to a nuclear magnetic resonance (NMR, Nuclear Magnetic Resonance) spectroscopy detection method, in particular to a method for obtaining a high-resolution NMR two-dimensional J-decomposition spectrum in an arbitrary inhomogeneous magnetic field environment based on multi-quantum coherence between heteronuclear molecules. Background technique [0002] NMR spectroscopy can provide information such as chemical shifts and J-coupling constants for material composition analysis and molecular structure analysis, and is widely used in biochemistry, materials science, and life science research. Among them, the one-dimensional NMR spectroscopy method has important applications in chemical analysis and biological metabolism detection due to its fast signal sampling and intuitive spectral information. However, the sample system with complex components will inevitably bring a large number of NMR signals, which show phenomena such as signal cro...

AI Technical Summary

Problems solved by technology

This method is based on the multi-quantum coherence between heteronuclear molecules to obtain high-resolution NMR two-dimensional J-decomposition spectrum in any inhomogeneous magnetic field environment (including large inhomogeneous magnetic field). The problem of the applicability of the resolution method in signal excitation and inhomogeneous magnetic field can recover high-resolution two-dimensional J-decomposition spectrum from any inhomogeneous magnetic field

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