Biological oil removal method for lubricating oil containing acid residues by using acidovorax avenae

Abstract

The invention provides acidovorax avenae (of which the collection number is GDMCC No: 60061) which achieves excellent oil removal performance, can take lubricating oil as a sole carbon source to grow and has an obvious degradation effect for all of high-concentration waste lubricating oil, waste engine oil, waste transformer oil and waste hydraulic oil. According to the invention, the oil removal characteristic of the strain is used, and a feasible biological treatment method is provided for acid residues produced in the regeneration process of the waste lubricating oil, the oil content of the treated acid residues can be effectively controlled, generally below 1-2.5mg / g, and multiple disadvantages and inapplicability of the previous physicochemical treatment are avoided, the method has the advantages of low treatment cost, good treatment effect, no secondary pollution, easy and convenient operation and the like. Waste recovery is carried out on the treated acid residues to effectively avoid environmental pollution, and save the treatment cost, so that the biological oil removal method disclosed by the invention makes a great contribution to orderly development of the waste lubricating oil regeneration treatment industry.

Description

technical field

[0001] The invention belongs to the technical field of biological degreasing treatment of acid slag produced in waste lubricating oil regeneration treatment process, and in particular relates to a method for biologically degreasing acid slag containing lubricating oil by using oat acidophilus bacteria. Background technique

[0002] With the further acceleration of the industrialization process, the demand for lubricating oil and the production of waste lubricating oil continue to increase. In view of the global energy shortage and strict environmental protection requirements, waste lubricating oil must be regenerated and reused, which has given birth to the vigorous development of the waste lubricating oil regeneration and disposal industry. The regeneration and disposal of waste lubricating oil has experienced the development of technical processes such as sulfuric acid-clay, atmospheric and vacuum distillation, solvent refining, molecular distillation, etc....

Examples

Embodiment 1

[0057] The method for biological degreasing of acid slag containing lubricating oil by using oat acidophilus comprises the following steps:

[0058] 1) Preparation of seed liquid: Inoculate oat acidophilus bacteria preserved on a slope into LB medium, and culture overnight for about 14 hours, as long as the culture liquid does not appear significantly turbid (generally OD 600nm =0.8-1.3); Inoculate into fresh LB medium with 0.5%-2% inoculum size for secondary activation culture, control the OD of the second generation culture solution 600nm =1.1-1.2, the second-generation culture solution is used as an inoculum to remove oil from acid residue; the whole culture process is cultivated on a shaking table at 35-37°C, 180rpm-200rom.

[0059] 2) Acid slag pretreatment: Spread the acid slag flat with a thickness of no more than 50cm, and evenly spray urea solution with a concentration of 3% (m / v) and 0.2mol / L dipotassium hydrogen phosphate solution on it, and the dosage is generally ...

Embodiment 2

[0062] Effects of different ratios of nitrogen and phosphorus nutrients on the effect of acid residue bioremoval.

[0063] Put about 4mL of secondary activated oat acidophilus fermentation liquid into the plastic culture cells labeled No. 1, No. 2, and No. 3 with about 50 g of acid slag, using urea as the nitrogen source and dipotassium hydrogen phosphate as the nitrogen source. Add nutrients to the phosphorus source according to the C:N:P ratio of 100:5:1, 100:10:1, and 100:15:1, and place it at 35°C for treatment. The plowing frequency is once every 24 hours. The water content of the acid residue is controlled at about 25%, and the oil content of the acid residue of the biologically treated waste lubricating oil refining acid residue is monitored at 7 days, 14 days, 21 days, and 30 days, and the degradation rate is calculated (oil removal rate in the figure).

[0064] Figure 8 It reflects the effect of using urea as the nitrogen source, dipotassium hydrogen phosphate as th...

Embodiment 3

[0066] Example of isothermal biological treatment under optimized conditions.

[0067]Inoculate about 4mL of oat acidophilus fermented broth after secondary activation into plastic culture grids labeled No. 1 and No. 2 with about 50 g of acid slag, using urea as nitrogen source and dipotassium hydrogen phosphate as phosphorus source, and C The ratio of :N:P is 100:15:1, the frequency of tillage is once every 12 hours, and the treatment is carried out at a constant temperature of 35°C. The water content of the acid slag is maintained at 30%, and the oat acidophilus is monitored at different treatment times. The oil content of the acid residue and calculate the degradation rate.

[0068] Figure 9 It reflects the biological oil removal of waste lubricating oil refining acid residue in a constant temperature environment of 35 °C. After 63 days of biological treatment, the acid residue with an initial oil content of 68.4 mg / g dropped to below 1 mg / g, and the biological oil remov...

technical field

[0001] The invention belongs to the technical field of biological degreasing treatment of acid slag produced in waste lubricating oil regeneration treatment process, and in particular relates to a method for biologically degreasing acid slag containing lubricating oil by using oat acidophilus bacteria. Background technique

[0002] With the further acceleration of the industrialization process, the demand for lubricating oil and the production of waste lubricating oil continue to increase. In view of the global energy shortage and strict environmental protection requirements, waste lubricating oil must be regenerated and reused, which has given birth to the vigorous development of the waste lubricating oil regeneration and disposal industry. The regeneration and disposal of waste lubricating oil has experienced the development of technical processes such as sulfuric acid-clay, atmospheric and vacuum distillation, solvent refining, molecular distillation, etc....

Examples

Embodiment 1

[0057] The method for biological degreasing of acid slag containing lubricating oil by using oat acidophilus comprises the following steps:

[0058] 1) Preparation of seed liquid: Inoculate oat acidophilus bacteria preserved on a slope into LB medium, and culture overnight for about 14 hours, as long as the culture liquid does not appear significantly turbid (generally OD 600nm =0.8-1.3); Inoculate into fresh LB medium with 0.5%-2% inoculum size for secondary activation culture, control the OD of the second generation culture solution 600nm =1.1-1.2, the second-generation culture solution is used as an inoculum to remove oil from acid residue; the whole culture process is cultivated on a shaking table at 35-37°C, 180rpm-200rom.

[0059] 2) Acid slag pretreatment: Spread the acid slag flat with a thickness of no more than 50cm, and evenly spray urea solution with a concentration of 3% (m / v) and 0.2mol / L dipotassium hydrogen phosphate solution on it, and the dosage is generally ...

Embodiment 2

[0062] Effects of different ratios of nitrogen and phosphorus nutrients on the effect of acid residue bioremoval.

[0063] Put about 4mL of secondary activated oat acidophilus fermentation liquid into the plastic culture cells labeled No. 1, No. 2, and No. 3 with about 50 g of acid slag, using urea as the nitrogen source and dipotassium hydrogen phosphate as the nitrogen source. Add nutrients to the phosphorus source according to the C:N:P ratio of 100:5:1, 100:10:1, and 100:15:1, and place it at 35°C for treatment. The plowing frequency is once every 24 hours. The water content of the acid residue is controlled at about 25%, and the oil content of the acid residue of the biologically treated waste lubricating oil refining acid residue is monitored at 7 days, 14 days, 21 days, and 30 days, and the degradation rate is calculated (oil removal rate in the figure).

[0064] Figure 8 It reflects the effect of using urea as the nitrogen source, dipotassium hydrogen phosphate as th...

Embodiment 3

[0066] Example of isothermal biological treatment under optimized conditions.

[0067]Inoculate about 4mL of oat acidophilus fermented broth after secondary activation into plastic culture grids labeled No. 1 and No. 2 with about 50 g of acid slag, using urea as nitrogen source and dipotassium hydrogen phosphate as phosphorus source, and C The ratio of :N:P is 100:15:1, the frequency of tillage is once every 12 hours, and the treatment is carried out at a constant temperature of 35°C. The water content of the acid slag is maintained at 30%, and the oat acidophilus is monitored at different treatment times. The oil content of the acid residue and calculate the degradation rate.

[0068] Figure 9 It reflects the biological oil removal of waste lubricating oil refining acid residue in a constant temperature environment of 35 °C. After 63 days of biological treatment, the acid residue with an initial oil content of 68.4 mg / g dropped to below 1 mg / g, and the biological oil remov...

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