Passive separation of whole blood

A platelet and storage technology, applied in the field of passive separation of whole blood, can solve the problems of expensive, labor-intensive, and energy-intensive

Inactive Publication Date: 2016-11-09
HALCYON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current centrifuge-based equipment for processing WB into blood fractions, especially for mobile blood collection vehicles, can be prohibitively expensive, bulky, labor-intensive, and energy-intensive
[0006] Additionally, high-speed centrifugation for WB isolation may subject blood cells to damaging physical forces and may require two centrifugation stages: separation of WB into concentrated RBC and platelet-rich plasma (PRP), followed by separation of PRP into PC and Platelet Poor Plasma (PPP)

Method used

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  • Passive separation of whole blood
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  • Passive separation of whole blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0166] Embodiment 1: CIF device is made

[0167] Desired device parameters were entered into custom-written MATLAB software, and the resulting design feature structural coordinates were exported for use in generating CAD for each device. A chrome-on-glass photomask designed by a CAD facility was then used to pattern the microchannel pattern in photoresist (SU8 3050, ~100-150 μm deep) that was spin-coated onto standard 4 on a silicon wafer and subjected to UV (i-line) exposure. An inverse polydimethylsiloxane (PDMS; SylGard 184, Dow Corning, Midland, MI) model of the wafer / photoresist master was created and passed Air plasma oxidation seals the model to a PDMS-coated glass slide. Input and output fluidic ports are created in ~5 mm thick PDMS by a biopsy punch prior to sealing. The particle suspension of interest is then introduced Previously, the PDMS device was treated with polyethylene glycol (PEG). Fluid was driven through the device by inserting an appropriate length of 1....

Embodiment 2A

[0169] Example 2A: CIF two-step design method for separation of polystyrene beads

[0170] In the first step, multiple filter channels are tested simultaneously, each with a different f 间隙 value. By observing that with f 间隙 changes in which particles are or are not maintained in the central channel, choosing an appropriate f 间隙 Values ​​for patterning the entire device in a second step.

[0171] Figure 11 The results obtained from performing the first step on beads with various diameters in three microchannel arrays with different central channel widths (100 μm, 125 μm and 150 μm) are presented. In each case, a parallel array of 33 test devices was designed to explore a wide range of degrees of filtration for each gap, where the device f 间隙 Values ​​range from 6.4x10 -5 (device #1) linearly down to 5.76x10 -4 (Device #33). A device array with given parameters was designed according to Equations 1 and 2 and created for testing. Visually by observing the f below which ...

Embodiment 2B

[0172] Example 2B: Application of a CIF two-step design based on polystyrene beads and platelet separation

[0173] The results in Example 2A give approximate guideline values ​​that can be used to study more complex particle enrichment / filtration applications than simple beads in saline solution. One such application of widespread interest in fact is the further enrichment of platelets in a suspension of PRP to levels above the AABB standard in PC. Platelets have an approximately discoid and highly variable, heterogeneous and dynamic shape with an effective diameter of ~1.5-4 μm. research flow as Figure 11 Behavior of the same three arrays of PRPs described in , with particular focus on devices in the range #2 to #10, which correspond to ~1-2x10 -4 the f 间隙 value. The platelets of the subjects studied were consistently retained in the following device #9 (w c =100μm), #8 (125μm) and #5 (150μm) in the central flow channel, the device corresponds to 1.92x10 -4 , 1.76x10 ...

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Abstract

Described are systems, methods, and kits for compression sedimentation and whole blood separation. For example, a compression sedimentation system may include a compression stage configured to accept a flexible reservoir configured to contain a liquid mixture. The compression stage may include a base substrate and a compression substrate configured to apply a force to the flexible reservoir effective to create a pressure in the liquid mixture. An apparatus for whole blood separation may include a sedimentation system that separates whole blood into a supernatant including platelet rich plasma and a subnatant including red blood cells. At least one platelet-concentrating device may be included to receive the supernatant including the PRP and to separate a platelet concentrate and a platelet poor plasma from the supernatant.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Patent Application No. 61 / 929,357, filed January 20, 2014, which is hereby incorporated by reference in its entirety. [0003] Statement Regarding Federally Sponsored Research [0004] This invention was made with United States Government support under Federal STTR Contract No. W81XWH-11-C-0008 awarded by the Department of Defense. The US Government may have certain rights in this invention. Background of the invention [0005] More than 30 million individual units of the three major blood components - red blood cells (RBC), platelet concentrate (PC) and plasma - are transfused annually in the United States. 70% of all whole blood (WB) approaching donation in the United States is collected on mobile blood donation vehicles, often more than 100 miles from centralized blood bank facilities. Due to significant differences in optimal storage conditions (1-6°C for RBC, 22±...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M1/00A61M1/02A61M1/34
CPCA61M1/3693A61M1/029B01L3/502746B01L3/502753B01L3/502761B01L2200/0652B01L2300/0681B01L2300/0864B01L2400/086A61M1/3695A61M1/0272A61M2202/0415A61M2202/0427A61M2202/0429A61M2202/0439B01D21/2444B01D21/28B01D21/006A61M1/3672B01D61/14
Inventor S·C·吉福德
Owner HALCYON BIOMEDICAL
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