Banlangen and general flavone combination and preparation method thereof

Abstract

The invention discloses a preparation method of a combination of total alkaloids of Sophora flavescens and total flavonoids of Sophora flavescens, comprising the following steps: step 1, crushing flavescens raw materials, sieving, extracting with an alcohol solution, and then combining the extracts; step 2, Centrifuge the obtained combined extracts to obtain the total flavonoids extraction filtrate, and retain the filter residue for later use; step 3, use the filtrate obtained in step 2 to absorb and elute with a macroporous adsorption resin, concentrate and dry to obtain the total flavonoids extract of Sophora flavescens; step 4, Extract the retained filter residue obtained for 2-3 times, and then combine the extracts; step five, concentrate the combined extracts obtained in step four under reduced pressure to obtain a supernatant; step six, absorb the obtained supernatant using a cation exchange resin After elution, alkalinize the resin with concentrated ammonia water, then elute with ethanol, collect the eluate, concentrate and dry to obtain the total alkaloid extract of Sophora flavescens. The preparation method of the invention can completely separate the total flavonoids and total alkaloids, and at the same time can take into account high extraction efficiency and simple and easy operation.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparation, in particular to a composition of total alkaloids of matrine and total flavonoids of matrine and a preparation method thereof. Background technique [0002] Sophora flavescens is the dried root of Sophora flavescens Ait., a perennial deciduous subshrub of the leguminous family Sophora genus. It has the effects of clearing heat and dampness, killing insects and diuresis, and can be used as bitter stomach invigorating agent, diuretic, anti-inflammatory drug, antidiarrheal drug and anthelmintic. [0003] The main functional components of Sophora flavescens are flavonoids and alkaloids. There are few existing techniques for simultaneously extracting flavescens flavonoids and total alkaloids of Sophora flavescens. Extract method", the method disclosed in this patent is to obtain the extract through the steps of extraction, filtration, concentration, drying and other s...

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Problems solved by technology

[0003] The main functional components of Sophora flavescens are flavonoids and alkaloids. There are few existing techniques for simultaneously extracting flavescens flavonoids and total alkaloids of Sophora flavescens. Extract method", the method disclosed in this patent is to obtain the extract through the steps of extraction, filtration, concentration, drying and other separation methods combined with macroporous resin, but this method does not involve further separation of Sophora flavescens alkaloids and flavonoids, Only the crude extracts of Sophora flavescens and Sophora flavescens should be obtained, and the purity is not high; another example is the patent "A method for producing Sophora flavescens and flavonoid salts", the method used in this patent is to extract with acid water first alkaloid until complete, and then use alcohol to extract flavonoids from Sophora flavescens, and the alcohol extract is processed to obtain flavonoids and flavonoid salts with higher purity. Mention of alkaloid purification steps yields only flavonoids and flavonoid salts

The patent number is "201110045288.0", and the title of the invention is "A joint preparation process of total flavonoids of Sophora flavescens and total alkaloids of Sophora flavescens", which discloses a further optimized extraction scheme, but this scheme still has the defects of insufficient extraction efficiency and cumbersome steps

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