A kind of method that utilizes citric acid slag to prepare composite cement retarder
A technology of citric acid slag and composite cement, applied in sustainable waste treatment, solid waste management, climate sustainability and other directions, can solve problems such as environmental pollution, property consumption, waste, etc., to reduce environmental pollution, realize reuse, Create simple effects
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example 1
[0017] In parts by weight, take 40 parts of EMB agar medium, 15 parts of ferrous gluconate solution with a mass fraction of 5%, 12 parts of ammonium nitrate, 2 parts of dipotassium hydrogen phosphate and 1.2 parts of potassium dihydrogen phosphate, stir evenly to form For the mixture, use hydrochloric acid with a mass fraction of 30% to adjust the pH of the mixture to 2.5, and then put the mixture into a pressure steam sterilizer for sterilization to obtain a culture medium; inoculate Thiobacillus ferrooxidans to the above-mentioned culture medium according to the inoculum size of 10%. Then move the culture medium to a constant temperature shaker, culture at 150r / min, 28°C for 4 days, use deionized water with a flow rate of 3mL / min to wash the surface of the culture medium until there is no hyphae on the surface, collect the washing liquid, the mycelia suspension was treated; according to the solid-to-liquid ratio of 1:2, take the citric acid slag and the above-mentioned myceli...
example 2
[0020] In parts by weight, take 50 parts of EMB agar medium, 10 parts of ferrous gluconate solution with a mass fraction of 5%, 8 parts of ammonium nitrate, 1 part of dipotassium hydrogen phosphate and 0.8 part of potassium dihydrogen phosphate, stir evenly to form For the mixture, use hydrochloric acid with a mass fraction of 30% to adjust the pH of the mixture to 3.5, and then put the mixture into a pressure steam sterilizer for sterilization to obtain a culture medium; inoculate Thiobacillus ferrooxidans to the above-mentioned culture medium according to the inoculum size of 15%. Then move the culture medium to a constant temperature shaker, culture at 150r / min, 32°C for 5 days, use deionized water with a flow rate of 5mL / min to wash the surface of the culture medium until there is no hyphae on the surface, collect the washing liquid, the mycelia suspension was treated; according to the solid-to-liquid ratio of 1:2, the citric acid residue and the mycelia suspension obtained...
example 3
[0023]In parts by weight, take 48 parts of EMB agar medium, 12 parts of ferrous gluconate solution with a mass fraction of 5%, 10 parts of ammonium nitrate, 2 parts of dipotassium hydrogen phosphate and 1.0 part of potassium dihydrogen phosphate, stir evenly to form For the mixture, use hydrochloric acid with a mass fraction of 30% to adjust the pH of the mixture to 3.0, and then put the mixture into a pressure steam sterilizer for sterilization to obtain a culture medium; inoculate Thiobacillus ferrooxidans to the above-mentioned culture medium according to the inoculum size of 12%. Then move the culture medium to a constant temperature shaker, culture at 150r / min, 30°C for 5 days, use deionized water with a flow rate of 4mL / min to wash the surface of the culture medium until there is no hyphae on the surface, collect the washing liquid, the mycelia suspension was treated; according to the solid-to-liquid ratio of 1:2, the citric acid residue and the mycelia suspension obtaine...
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