Method for extracting and purifying beta-glucan from highland barley grains

A glucan and grain technology, applied in the field of extracting and purifying beta-glucan, can solve problems such as differences in the ratio of glycosidic bonds, and achieve the effects of reducing material resource consumption, good effect, and time saving

Active Publication Date: 2016-11-16
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical structures of β-glucans in cereals are similar, but the ratios of β-(1→3) and β-(1→4) glycosidic bonds in the molecules are different

Method used

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  • Method for extracting and purifying beta-glucan from highland barley grains
  • Method for extracting and purifying beta-glucan from highland barley grains

Examples

Experimental program
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Effect test

Embodiment 1

[0032] This example provides a method for extracting and purifying β-glucan from highland barley grains, the process is as follows figure 1 As shown, the specific steps are as follows:

[0033] Sorting several normal highland barley grains without lesions, grinding and passing through a 0.4mm sieve; weigh 10g of powdered samples and add 90mL of distilled water to dissolve them, add 50μL of high-temperature-resistant α-amylase, and bathe in 80°C water for 1 hour; adjust the temperature of the water bath to 60°C , add 15mg of papain, water bath for 1h; inactivate enzyme in 100℃ water bath for 10min, add 400μL glucoamylase after cooling, 60℃ water bath for 1h; inactivate enzyme in 100℃ water bath for 10min, after cooling in 86℃ water bath for 4h; The precipitate was saved and the supernatant was used for later use.

[0034] Slowly add 3 times the volume of absolute ethanol dropwise to the supernatant under the condition of magnetic stirring, and let it stand overnight; centrifug...

Embodiment 2

[0039] This example provides a method for extracting and purifying β-glucan from highland barley grains, which is basically the same as the method in Example 1, the only difference is that the elution flow rates of DE-32 and S-400 are different, that is, the implementation The elution flow rates of DE-32 and S-400 in Example 2 are both 0.5mL / min.

[0040] The β-glucan filtrate collected in this example contains a large amount of glucose according to the sugar composition measured by gas chromatography. Further, glucose was identified by using a β-1,3 glucan detection kit, and the result showed that the glucose in the filtrate was β-glucan. According to the detection results of liquid chromatography, it can be seen that the purity of the β-glucan prepared in this example is 97.63%, and the extraction rate of the β-glucan obtained after elution of the crude sample obtained after enzymolysis is 11.6%. Compared with Example 1, the purity of this example is equivalent to that of Exa...

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Abstract

The invention discloses a method for extracting and purifying beta-glucan from highland barley grains. The method comprises the following steps: taking the highland barley grains as raw materials; grinding, sieving, carrying out enzymolysis, carrying out alcohol precipitation, carrying out vacuum freeze drying and degreasing to obtain a coarse sample; and after dissolving the coarse sample, eluting with packing cellulose DE-32 and S-400 successively to obtain a purified sample. By the method, the high-purity beta-glucan can be effectively separated from the highland barley grains, the extraction rate of the beta-glucan in the coarse sample can reach 31.1%, and the purity can reach 98.86%.

Description

technical field [0001] The invention belongs to the field of food, and in particular relates to a method for extracting and purifying beta-glucan from highland barley grains. Background technique [0002] β-glucan (β-glucan) is a linear unbranched viscous polysaccharide, and its isocarban glycosidic linkage is β-type, through β-(1→3) and β-(1→4) glycosidic bonds β-D-Glucopyranose units are connected and widely distributed in the kingdoms of animals, plants and microorganisms. The chemical structures of β-glucans in grains are similar, but the ratios of β-(1→3) and β-(1→4) glycosidic bonds in the molecules are different. The water solubility of β-glucan is related to the ratio of these two glycosidic bonds. In general, the ratio of β-(1→4) glycosidic linkages to β-(1→3) glycosidic linkages in water-soluble β-glucans is 2.3:1, whereas in non-water-soluble β-glucans this ratio is 2.3:1. The ratio is higher. According to reports, β-glucan can: 1) lower serum cholesterol and ...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0024
Inventor 隋中泉卢飞廖樟华白铠豪钟金锋
Owner SHANGHAI JIAO TONG UNIV
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