SNP (single nucleotide polymorphism) combination, detection method and kit for detecting liver damage susceptible genotype of antitubercular drug

A susceptibility gene and detection method technology, applied in the field of medical molecular biology diagnosis, can solve the problems of high throughput, complicated operation and long cycle of chip method

Active Publication Date: 2016-11-16
THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY +1
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Problems solved by technology

The direct sequencing method is the "gold standard", but the operation is complicated, the cost is high, and the cycle is long; the chip method has high throughput and is suitable for the detection of large samples, but the accuracy is poor; the mass spectrometry method has high accuracy

Method used

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  • SNP (single nucleotide polymorphism) combination, detection method and kit for detecting liver damage susceptible genotype of antitubercular drug
  • SNP (single nucleotide polymorphism) combination, detection method and kit for detecting liver damage susceptible genotype of antitubercular drug
  • SNP (single nucleotide polymorphism) combination, detection method and kit for detecting liver damage susceptible genotype of antitubercular drug

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Experimental program
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Embodiment 1

[0088] 1. SNP sequence screening and confirmation

[0089] Perform PCR amplification on the SNP site sequences (as shown in Sequence Table 1) of DNA samples of 591 cases without liver damage (i.e. the normal control group) and 324 cases of liver damage (i.e. the case group), and amplify the obtained PCR products Separate by electrophoresis on 1.5% agarose gel, recover and purify the target band for sequencing.

[0090] Use the online Shesis software (http: / / analysis.bio-x.cn / SHEsisMain.htm) to perform statistics on the allele frequency and genotype frequency of the SNP loci found in the sequencing, and conduct case studies for alleles and genotypes Chi-square test and calculation of odds ratio (odds ratio), inter-locus linkage analysis, haploid analysis and other statistical analysis of genetics in the control group and normal control group, a total of 7 SNPs significantly related to liver injury caused by anti-tuberculosis drugs were obtained. Points include: rs1801279(191G>...

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Abstract

The present invention relates to an SNP (single nucleotide polymorphism) combination, detection method and kit for detecting liver damage susceptible genotype of an antitubercular drug and belongs to the technical field of medical molecular biological diagnosis; the SNP combination includes 7 SNP sites, and nucleotide sequences of the 7 SNP sites are shown sequentially as in SEQ ID NO. 1-7; the present invention also relates to an SNP detection method, comprising PCR (polymerase chain reaction) amplification and double-labeled probe melting curve analytical reaction, and primer pairs and double-labeled probe sequences for detection of the 7 SNP sites are shown as in SEQ ID NO. 8-20. The SNP site combination, detection method and kit provided herein enables quick, accurate, simple and high-throughput detection for a patient's genotype and prediction for the liver damage risk due to the patient using the antitubercular drug.

Description

technical field [0001] The invention relates to single nucleotide polymorphism (SNP) detection, in particular to a SNP combination, a detection method and a kit for detection of susceptibility genotypes of liver damage of anti-tuberculosis drugs, belonging to the technical field of medical molecular biology diagnosis. Background technique [0002] Tuberculosis remains a serious public health problem, especially in developing countries. According to WHO statistics, 1 / 3 of the world's population is infected with Mycobacterium tuberculosis (MTB), which is the second largest killer of infectious diseases in the world. my country is one of the 22 countries with a high burden of tuberculosis in the world, and the number of active tuberculosis patients ranks second in the world. At present, the infection rate of Mycobacterium tuberculosis in all age groups in my country is 44.5%, and about 550 million people in the country are infected by Mycobacterium tuberculosis. [0003] Curr...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156C12Q2600/16
Inventor 吴雪琼张俊仙孙伟民王杰安慧茹殷铬俊梁艳阳幼荣白雪娟
Owner THE 309TH HOSPITAL OF CHINESE PEOPLES LIBERATION ARMY
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