Quick PCR (polymerase chain reaction) amplifier temperature control method

Abstract

The invention discloses a quick PCR (polymerase chain reaction) amplifier temperature control method and relates to the field of DNA amplification. The method includes that a heating mechanism at an air incoming position of an air drum in a fan component is started for heating, air heated by the heating mechanism is blown by the air drum to flow inside a shell and form a heat circulating air field, and a PCR pipe is heated by the air in the heat circulating air field; during cooling, the fan component outputs air, an air incoming door and an air outgoing door are opened, and cold air outside the shell enters the shell from the air incoming door and then is blown by the fan component to circularly flow inside the shell and then discharged from the air outgoing door. By using the method, heating and cooling rate of a PCR amplifier is increased, PCR amplification time is shortened effectively, and PCR amplification efficiency is high; probability that miscellaneous bands appear in experiments can be reduced, improving of experiment result accuracy is facilitated, and safety coefficient is high.

Description

technical field [0001] The invention relates to the field of DNA amplification, in particular to a temperature control method for a rapid PCR amplification instrument. Background technique [0002] Polymerase Chain Reaction (PCR) is an in vitro nucleic acid amplification technology developed in the mid-1980s. It has outstanding advantages such as specificity, sensitivity, high yield, rapidity, simplicity, good repeatability, and easy automation. The principle is similar to the in vivo replication of DNA, except that a suitable condition is provided for the in vitro synthesis of DNA in the PCR tube. After years of development, this technology has become very mature, and it is now the most important and commonly used technology in the field of molecular biology research and clinical diagnosis. For example, it has been widely used in the diagnosis of genetic diseases, the detection of nucleic acid of pathogens in clinical samples, the genetic identification of forensic samples...

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the temperature change of the PCR amplification instrument is accomplished by the semiconductor refrigeration sheet and the heat conduction module. After the temperature is collected by the temperature measuring probe in the heat conduction module, the temperature is directly controlled by the PID temperature control method, and the temperature of the heat conduction module reaches the target temperature. However, the temperature rise of the solution in the PCR tube has hysteresis, and as the temperature of the solution in the PCR tube approaches the target temperature, the temperature difference between the inside and outside becomes smaller and smaller, and the temperature rises slower and slower. Each time the temperature rises or cools down, it takes tens of seconds , it takes about 2.5 hours to do 30 PCR cycles, and its maximum heating and cooling rate is 2-3 degrees per second. This increases the miscellaneous bands in the experiment, which affects the accuracy of the experimental results; on the other hand, when the PCR tube is placed in the heat conduction module for heat transfer, the PCR tube cannot be tightly attached to the tube wall and the heat conduction module, and there will be a gap between the two , and the thermal conductivity of air is very low. Under the same test conditions, the results of different PCR tubes will be different, which will affect the accuracy of the test results.

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