Method for measuring surface temperature of gold nanorod, heat transmission device constructed by using gold nanorod, and application

A gold nanorod and surface temperature technology, applied in the measurement of the surface temperature of gold nanorods, a heat transfer device for the surface temperature field of gold nanorods, and the field of nucleic acid amplification based on nano-localized heating

Pending Publication Date: 2021-05-14
SUZHOU DIYINAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The measurement method can accurately measure the nano-localized temperature around the gold nanoparticles, and solves the problem of how to measure and control the nano-localized temperature around the gold nanoparticles
At the same time, using the constructed heat transfer model, the design of DNA probes with spacers and reaction regions solved the problem of how to limit nucleic acid amplification to the nanolocal environment around gold nanoparticles

Method used

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  • Method for measuring surface temperature of gold nanorod, heat transmission device constructed by using gold nanorod, and application
  • Method for measuring surface temperature of gold nanorod, heat transmission device constructed by using gold nanorod, and application
  • Method for measuring surface temperature of gold nanorod, heat transmission device constructed by using gold nanorod, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] In this example, the instability of the gold-sulfur bond is used to provide an indirect method for measuring the surface temperature of AuNRs.

[0059] In order to clarify the relationship between the surface temperature of AuNRs and the dissociation of Au-S bonds, in this example, AuNRs-DNA was dispersed in a Tris buffer with a pH of 8, wherein the concentration of gold nanorods was 50 pM and the concentration of surface modification probes was 0.1 ×10 12 molecules / cm 2 (molecule / cm 2 ), and heat the entire solution to the design temperature using a dry bath. When the whole solution reaches a constant temperature, it is assumed that the surface temperature of AuNRs is the same as the solution temperature, and the solution temperature can be measured by an infrared thermal imaging camera.

[0060] Since the ends of the DNA probes have been modified with organic fluorescent dyes, the recovery of fluorescence can be observed when the DNA probes are released from the su...

Embodiment 2

[0067] This example was used to determine the effect of the power intensity of the incident laser light on the temperature gradient around AuNRs. Among them, the power intensity of the incident laser directly affects the input energy and the heat generated by AuNRs, resulting in the change of the surface temperature.

[0068] 46 pM AuNRs-DNA probes were dispersed in Tris buffer at pH 8, where the DNA probes were thiolated DNA probes consisting of a reaction region and a spacer region, where the reaction region was responsible for pairing with the target NA and initiating NA amplification , the polyadenine sequence in the spacer can adjust the distance from the reaction zone to the surface of AuNRs; and with five different intensities (7.17mW / mm 2 、9.55mW / mm 2 、14.51mW / mm 2 、16.99mW / mm 2 and 38.22mW / mm 2 ) with 808nm laser for excitation.

[0069] Such as image 3 As shown, the solution temperature increased sharply at the beginning and then reached a constant temperature...

Embodiment 3

[0074] This example was used to determine the effect of the solution concentration of AuNRs on the temperature gradient around AuNRs. Among them, the solution concentration of AuNRs will affect the absorption efficiency and scattering between incident light and AuNRs.

[0075] In order to understand the relationship between temperature field and AuNRs-DNA concentration, different concentrations of AuNRs-DNA probes were dispersed in Tris buffer at pH 8 and heated with 38.22 mW / mm 2 808nm laser for excitation.

[0076] Such as Figure 6 As shown, the rising trend of the solution temperature is similar to that at different laser intensities, and the solution temperature reaches the highest point at 90 s, and then remains almost constant except for a slight decrease in the two highest concentrations (i.e., 92 pM and 46 pM).

[0077] Such as Figure 7 As shown, after 40 min of incubation, the fraction of DNA released at different AuNR-DNA probe concentrations was obtained, and t...

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Abstract

The invention provides a method for measuring the surface temperature of a gold nanorod, a heat transmission device constructed by using the gold nanorod, and application. The measuring method comprises the following steps: connecting a DNA probe to the surface of the gold nanorod through a gold-sulfur bond, dispersing in an alkaline buffer solution, and heating by using laser; and enabling the DNA probe to release fluorescence from the surface of the gold nanorod and emit fluorescence, detecting the fluorescence intensity of the DNA probe, and calculating the surface temperature of the gold nanorod according to the fluorescence intensity. The nanometer local temperature around gold nanoparticles is measured by utilizing the instability of gold-sulfur bond, the heat transmission device of a gold nanorod surface temperature field is constructed, and meanwhile, the length of a spacer region and a reaction region of the DNA probe can be designed and nucleic acid amplification can be carried out through the device; and by utilizing the method, the volume of a PCR reaction system can be obviously reduced, the temperature increasing and decreasing rate in the nucleic acid amplification process is greatly improved, and the time required by the amplification reaction is shortened.

Description

technical field [0001] The invention belongs to the field of nucleic acid amplification and detection, and in particular relates to a method for measuring the surface temperature of gold nanorods, a heat transfer device constructed by using the same and an application thereof, in particular to a method for measuring the surface temperature of gold nanorods, a gold nanorod surface Heat transfer device of temperature field and nucleic acid amplification method based on nanometer localized heating. Background technique [0002] Nucleic acid amplification tests (NAATs) have been widely used in the biomedical field and become an indispensable detection method for the diagnosis of cancer, viral and bacterial infections, food safety detection and environmental monitoring. In the field of nucleic acid detection, polymerase chain reaction (PCR) technology has become the gold standard of NAATs due to its high sensitivity and specificity, and easy operation. [0003] PCR technology re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01K11/32G01N21/64C12Q1/686
CPCG01K11/32G01K11/3213G01N21/6428G01N21/6486C12Q1/686C12Q2563/157C12Q2527/101C12Q2563/107
Inventor 游民黎徐峰佟昊阳
Owner SUZHOU DIYINAN BIOTECHNOLOGY CO LTD
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