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Pseudorabies live vaccine lipidosome dilution liquid lyophilized product and preparation method thereof

A technology for pseudorabies and freeze-dried products, which is applied in the field of freeze-dried products of liposome diluent of live pseudorabies vaccine and its preparation, can solve problems that restrict the healthy development of the pig industry, extend the protection period of vaccines, and reduce the immune gap Period, the effect of enhancing the immunity of pigs

Inactive Publication Date: 2016-11-23
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In developed countries such as Europe and the United States, thanks to the application of PRV gene-deleted live vaccines and corresponding identification methods, the disease has been controlled or eradicated; however, in developing countries, the disease still occurs frequently, which seriously restricts the development of the pig industry in these countries and regions. healthy growth

Method used

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  • Pseudorabies live vaccine lipidosome dilution liquid lyophilized product and preparation method thereof
  • Pseudorabies live vaccine lipidosome dilution liquid lyophilized product and preparation method thereof
  • Pseudorabies live vaccine lipidosome dilution liquid lyophilized product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of liposome

[0021] Weigh 4 g of soybean lecithin and 3 g of glycocholic acid, dissolve them with 500 ml of chloroform, and mix them uniformly, and remove the chloroform in the solution by decompression to prepare liposome membranes; 200ml of benzyl acid and potassium citrate buffer solution, this buffer solution is added in the liposome membrane solution, is prepared into liposome with homogenizer after dehydration.

Embodiment 2

[0022] Embodiment 2: the preparation of liposome

[0023] Weigh 3 g of soybean lecithin and 2 g of glycocholic acid, dissolve them with 400 ml of chloroform, mix them evenly, and remove the chloroform in the solution by decompression to prepare liposome membranes; 100ml of benzyl acid and potassium citrate buffer solution, this buffer solution is added in the liposome membrane solution, is prepared into liposome with homogenizer after dehydration.

Embodiment 3

[0024] Embodiment 3: Preparation of pH 7 phosphate buffer

[0025] Take 8g of sodium chloride, 0.3g of potassium chloride, 1g of disodium hydrogen phosphate, 0.3g of potassium dihydrogen phosphate, 0.3g of calcium chloride, and 0.1g of magnesium chloride containing 6 crystal water; after mixing the above ingredients, dissolve them in 1000ml of double distilled water, after 116 ℃, high pressure sterilization for 30 minutes.

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Abstract

The invention relates to a freeze-dried product of liposome diluent of live pseudorabies vaccine and a preparation method thereof, belonging to the technical field of veterinary biological products. It contains the following components per 1000ml of PBS: 1-6g of pinocinol, 2-8g of procurdin, 5-10g of daidzein, 1-3g of paeoniflorin, 5-10g of liposome and 5-15g of lyoprotectant. The invention has the following beneficial effects: 1) The freeze-dried product of a liposome diluent of a pseudorabies live vaccine prepared by the invention can make pigs produce antibodies in advance and reduce the blank period of immunity. 2) The freeze-dried product of a liposome diluent of live pseudorabies vaccine prepared by the present invention can not only enhance the immunity of pigs, but also prolong the protection period of the vaccine and improve the immune effect of the live vaccine of pseudorabies.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a freeze-dried product of liposome diluent of live pseudorabies vaccine and a preparation method thereof. Background technique [0002] Pseudorabies virus (PRV) belongs to herpesvirus (Herpesviridae) α herpesvirus subfamily (Alphaher-pesririnae) varicella virus (Varicello virus) herpesvirus subfamily type Ⅰ (Porcine herpesvirus Typel), can cause severe pigs disease. At the same time, pigs are not only the reservoir of pseudorabies virus, but also the source of infection of pseudorabies virus. Controlling swine pseudorabies is of great significance not only to the pig breeding industry itself, but also to the smooth development of other animal breeding. In developed countries such as Europe and the United States, thanks to the application of PRV gene-deleted live vaccines and corresponding identification methods, the disease has been controlled ...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K9/127A61K47/26A61K47/22A61K39/245A61P31/22
CPCA61K9/19A61K9/127A61K39/12A61K47/22A61K47/26A61K2039/5254A61K2039/552C12N2710/16734
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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