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Yak collagen preparation method

A technology of collagen and yak, which is applied in the field of collagen, can solve the problems of complex collagen methods and achieve the effect of simple preparation methods

Inactive Publication Date: 2016-11-23
夏佳文 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the method of producing collagen from yak is relatively complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The preparation method of yak collagen of the present invention comprises the following steps:

[0028] (1) crushing the yak tissue;

[0029] (2) enzymatically hydrolyzing the yak tissue through step (1) to obtain interstitial fluid;

[0030] (3) separating the collagen liquid from the interstitial fluid;

[0031] (4) Purifying the collagen liquid to obtain a collagen solid

[0032] The above-mentioned yak tissue can be connective tissue, for example, it can be obtained from one of yak skin, yak hoof, yak scalp and yak bone or any combination thereof.

[0033] In order to obtain higher collagen purity, the yak tissue can also be degreased before the pulverization in step (1). Here, the function of degreasing treatment is to remove the fat in the yak tissue. The degreasing method can adopt lye, where lye includes sodium hydroxide solution, potassium hydroxide solution, sodium bicarbonate solution or any mixture thereof. Degreasing with lye can be done by dipping, an...

Embodiment 1

[0049] Step 1. After washing the yak tissue (such as yak skin, yak hoof, yak scalp, yak bone, etc.), put it into a tissue pulverizer (3000rpm rpm, temperature -10°C) for tissue pulverization, and pulverize for 15min to obtain Tissue minced.

[0050] Step 2: Put the minced tissue in an enzymatic hydrolysis tank, add pepsin, trypsin, papain, and neutral protease to carry out enzymatic hydrolysis reaction, and obtain interstitial fluid (the temperature is controlled at -10°C);

[0051] Step 3: Centrifuge the tissue fluid in a low-temperature high-speed centrifuge (11,000 rpm, temperature -10° C.) for 10 minutes to obtain a supernatant, and filter residue for another use.

[0052] Step 4. Place the supernatant in a protein chromatography (pressure 0.08 MPa, temperature -10° C.) to obtain a chromatographic solution.

[0053] Step 5, placing the chromatographic solution in a lyophilizer for drying (temperature -70° C.) to obtain lyophilized crystals.

[0054] Step 6. Weigh the cry...

Embodiment 2

[0056] Step 1. After washing the yak tissue (such as yak skin, yak hoof, yak scalp, yak bone, etc.), put it into a tissue pulverizer (5000rpm rpm, temperature 0°C) for tissue pulverization, pulverize for 5min, and obtain tissue mince.

[0057] Step 2: Put the minced tissue in an enzymatic hydrolysis tank, add pepsin, trypsin, papain, and neutral protease to carry out enzymatic hydrolysis reaction, and obtain interstitial fluid (the temperature is controlled at 0°C);

[0058] Step 3: Centrifuge the tissue fluid in a low-temperature high-speed centrifuge (13,000 rpm, temperature -10°C) for 1 min to obtain a supernatant, and filter residue for another use.

[0059] Step 4. Place the supernatant in a protein chromatography (pressure 0.08 MPa, temperature -10° C.) to obtain a chromatographic solution.

[0060] Step 5, placing the chromatographic solution in a lyophilizer for drying (temperature -70° C.) to obtain lyophilized crystals.

[0061] Step 6. Weigh the crystals and put t...

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PUM

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Abstract

The invention discloses a yak collagen preparation method. The yak collagen preparation method comprises the steps of smashing yak tissue, then carrying out enzymolysis, separating the enzymolytic interstitial fluid and purifying to obtain the collagen solid. Thus, the yak collagen preparation method is simple.

Description

technical field [0001] The invention relates to the technical field of collagen, in particular to a preparation method of yak collagen. Background technique [0002] Collagen is a biological polymer, the most abundant and widely distributed functional protein in mammals, accounting for 25% to 30% of the total protein, and even as high as 80% in some organisms. Collagen has been widely used in food, medicine, tissue engineering, cosmetics and other fields due to its good biocompatibility, biodegradability and biological activity. For example, "Ejiao", a traditional Chinese collagen health product, is mainly made of donkey skin (actually the collagen contained in donkey skin), which has a high nourishing and health-care effect, and is known as one of the three treasures of traditional Chinese medicine. [0003] In the prior art, the method for preparing collagen from yak is relatively complicated. Contents of the invention [0004] In view of this, one aspect of the presen...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/16
CPCC12P21/06C07K14/78
Inventor 夏佳文原林周萍平
Owner 夏佳文
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