Method for preparing dark rice protein peptide and application of dark rice protein peptide

Abstract

The invention discloses a method for preparing dark rice protein peptide and application of the dark rice protein peptide. The dark rice protein peptide is prepared from, by weight, 10 parts of dark rice, 0.1-1 part of amylase, 0.03-0.1 part of protease and 1.0-5.0 parts of activated carbon. The dark rice is soaked for 1-6 h with deionized water and then ground in a colloid mill to form powder with the particle size being 3-100 micrometers; amylase is added into the powder; precipitate is obtained through centrifugal draining and washed with deionized water; the washed precipitate and the deionized water are evenly mixed according to the weight part ratio of 1:10-1:15, and the PH value is adjusted by a NaO solution to be alkalescent; protease is added, and heat is preserved for 8-24 h at 45-55 DEG C. The ultrafine homogenization and composite enzymatic hydrolysis technology is adopted, safety hidden hazards caused by traditional physical and chemical technologies are avoided, and generation of toxic byproducts like lysinoalanine is prevented. The method has the advantages that the molecular weight is small, solubility is high, and absorption is easy, and the antibacterial rate of the dark rice protein peptide prepared through the method to escherichia coli reaches 99.98%.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a preparation method and application of umein peptide. Background technique [0002] Umi is made by soaking and steaming the fresh leaf juice of black rice tree. "Compendium of Materia Medica" records: "Black rice, the rice grains are tight and small, black like pearls, sweet, flat, non-toxic. Daily intake and one combination, no hunger, good color, strong bones and muscles, can work. Benefit the stomach, nourish the marrow, Eliminate the three insects, long-term clothing will turn white but old.” Since the Southern and Northern Dynasties, residents in the middle and lower reaches of the Yangtze River have always had the custom of eating black rice on the eighth day of the fourth lunar month every year, and it has lasted for more than 1,000 years. my country's understanding of black rice is limited to ordinary folk food, and its in-depth development and utilization rate i...

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Problems solved by technology

However, these synthetic drugs have certain side effects on the human body and will produce obvious drug resistance after long-term use, resulting in no obvious inhibitory effect on Escherichia coli

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